Proteintech

Occludin Monoclonal Antibody (1D3C4)

Occludin Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 10

Occludin Antibody (66378-1-IG) in ICC/IF

Immunofluorescent analysis of (-20°C Ethanol) fixed Caco-2 cells using Occludin antibody (Product # 66378-1-IG, Clone: 1D3C4 ) at dilution of 1:1600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Occludin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

Product Details

66378-1-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:6,000

View 35 publications 35 publications

Immunohistochemistry (IHC)

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:1,600

View 19 publications 19 publications

Product Specifications

Species Reactivity

Human, Mouse, Pig, Rat

Published species

Not Applicable

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

1D3C4

Immunogen

Occludin Fusion Protein Ag4057 (269-522 aa encoded by BC029886)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: RKMDRYDKS NILWDKEHIY DEQPPNVEEW VKNVSAGTQD VPSPPSDYVE RVDSPMAYSS NGKVNDKRFY PESSYKSTPV PEVVQELPLT SPVDDFRQPR YSSGGNFETP SKRAPAKGRA GRSKRTEQDH YETDYTTGGE SCDELEEDWI REYPPITSDQ QRQLYKRNFD TGLQEYKSLQ SELDEINKEL SRLDKELDDY REESEEYMAA ADEYNRLKQV KGSADYKSKK NHCKQLKSKL SHIKKMVGDY DRQKT (269-522 aa encoded by BC029886)

Target Information

Occludin is a 65 kDa protein that can exist in a variety of phosphorylated forms, ranging up to approximately 82 kDa. Occludin is thought to be involved in regulating both the localization and the function of occludin. Polyunsaturated fatty acids are known to up-regulate occludin expression, increasing the transendothelial cell resistance and reducing the cellular permeability to large molecules. The level of occludin varies greatly depending on tissue; in brain tissue, occludin is highly and continuously expressed at cell-cell contact sites, whereas non-neural tissues show lower expression and discontinuous distribution. Overall structural features of the occludin protein are highly conserved in all the species examined. Under-expression of tight junction proteins, including occludin, is a key molecular abnormality responsible for the increased permeability of tumor endothelial tight junctions, which contributes to brain tumor edemas.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Occludin; phosphatase 1, regulatory subunit 115; tight junction protein occludin

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Gene Aliases: AI503564; BLCPMG; Ocl; OCLN; PPP1R115

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UniProt ID: (Human) Q16625, (Mouse) Q61146, (Rat) Q6P6T5

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Entrez Gene ID: (Pig) 397236, (Human) 100506658, (Mouse) 18260, (Rat) 83497

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Function(s)

protein binding thiopurine S-methyltransferase activity protein domain specific binding S-adenosylmethionine-dependent methyltransferase activity structural molecule activity general transcription factor

Process(es)

protein complex assembly methylation cell-cell junction organization S-adenosylhomocysteine metabolic process S-adenosylmethionine metabolic process bicellular tight junction assembly

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Occludin Monoclonal Antibody (1D3C4)

Occludin Antibody (66378-1-IG) in ICC/IF

Product Details

66378-1-IG

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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