Invitrogen

Occludin Monoclonal Antibody (OC-3F10), FITC

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

3 Published Figures

11 References

View all (58) Occludin antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Occludin Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 7

Occludin Antibody (33-1511) in ICC/IF

Knockdown of Occludin was achieved by transfecting Caco-2 cells with Occludin specific siRNA (Silencer® select Product # s458015 and s531865). Immunofluorescence analysis was performed on untransfected Caco-2 cells (panels a, d), transfected with non-specific scrambled siRNA (panels b,e) and transfected with Occludin specific siRNA (panel c,f). Cells were fixed, permeabilized, and labelled with Occludin Monoclonal Antibody (OC-3F10), FITC (Product # 33-1511, 5 µg/mL). Nuclei (blue) were stained using ProLong™ Diamond Antifade Mountant with... View More

Occludin Antibody in Flow Cytometry (Flow)

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Product Details

33-1511

Applications

Tested Dilution

Publications

Western Blot (WB)

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

5 µg/mL

View 6 publications 6 publications

Flow Cytometry (Flow)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Dog, Human, Mouse, Rat

Published species

Human, Mouse, Non-human primate, Rabbit, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

OC-3F10

Immunogen

GST fusion protein consisting of the C-terminal region (~150aa) of human occludin.

View immunogen

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 50% glycerol, 1% BSA

Contains

0.1% sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533102

Target Information

Occludin is a 65 kDa protein that can exist in a variety of phosphorylated forms, ranging up to approximately 82 kDa. Occludin is thought to be involved in regulating both the localization and the function of occludin. Polyunsaturated fatty acids are known to up-regulate occludin expression, increasing the transendothelial cell resistance and reducing the cellular permeability to large molecules. The level of occludin varies greatly depending on tissue; in brain tissue, occludin is highly and continuously expressed at cell-cell contact sites, whereas non-neural tissues show lower expression and discontinuous distribution. Overall structural features of the occludin protein are highly conserved in all the species examined. Under-expression of tight junction proteins, including occludin, is a key molecular abnormality responsible for the increased permeability of tumor endothelial tight junctions, which contributes to brain tumor edemas.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Early and late genome-wide gastric epithelial transcriptome response during infection with the human carcinogen Helicobacterpylori.

Cell insight

Sharafutdinov I,Ekici A,Vieth M,Backert S,Linz B

33-1511 was used in Immunocytochemistry-immunofluorescence to suggest that H. pylori disturbs previously unknown molecular pathways in an HtrA-dependent and HtrA-independent manner.

Wed Jun 01 00:00:00 EDT 2022

TRPV6 channel mediates alcohol-induced gut barrier dysfunction and systemic response.

Cell reports

Meena AS,Shukla PK,Bell B,Giorgianni F,Caires R,Fernández-Peña C,Beranova S,Aihara E,Montrose MH,Chaib M,Makowski L,Neeli I,Radic MZ,Vásquez V,Jaggar JH,Cordero-Morales JF,Rao R

33-1511 was used in Immunohistochemistry to identify TRPV6, a Ca2+-permeable channel, as responsible for alcohol-induced elevation of intracellular Ca2+, intestinal barrier dysfunction, and systemic inflammation.

Tue Jun 14 00:00:00 EDT 2022

Comparative Analysis of Cell-Cell Contact Abundance in Ovarian Carcinoma Cells Cultured in Two- and Three-Dimensional In Vitro Models.

Biology

Kutova OM,Sencha LM,Pospelov AD,Dobrynina OE,Brilkina AA,Cherkasova EI,Balalaeva IV

Published figure using Occludin monoclonal antibody (Product # 33-1511) in Flow Cytometry

Fri Dec 04 00:00:00 EST 2020

Comparative Analysis of Cell-Cell Contact Abundance in Ovarian Carcinoma Cells Cultured in Two- and Three-Dimensional In Vitro Models.

Biology

Kutova OM,Sencha LM,Pospelov AD,Dobrynina OE,Brilkina AA,Cherkasova EI,Balalaeva IV

Published figure using Occludin monoclonal antibody (Product # 33-1511) in Flow Cytometry

Fri Dec 04 00:00:00 EST 2020

Dual action of highbush blueberry proanthocyanidins on Aggregatibacter actinomycetemcomitans and the host inflammatory response.

BMC complementary and alternative medicine

Ben Lagha A,LeBel G,Grenier D

Published figure using Occludin monoclonal antibody (Product # 33-1511) in Immunofluorescence

Wed Jan 10 00:00:00 EST 2018

Long-Term In Vitro Expansion of Epithelial Stem Cells Enabled by Pharmacological Inhibition of PAK1-ROCK-Myosin II and TGF-β Signaling.

Cell reports

Zhang C,Lee HJ,Shrivastava A,Wang R,McQuiston TJ,Challberg SS,Pollok BA,Wang T

33-1511 was used in Immunocytochemistry-immunoflourescence to develop a method for one trillion-fold expansion of human epithelial stem and progenitor cells from skin, airway, mammary, and prostate glands in the absence of feeder cells.

Tue Oct 16 00:00:00 EDT 2018

TRPV4 Regulates Tight Junctions and Affects Differentiation in a Cell Culture Model of the Corneal Epithelium.

Journal of cellular physiology

Martínez-Rendón J,Sánchez-Guzmán E,Rueda A,González J,Gulias-Cañizo R,Aquino-Jarquín G,Castro-Muñozledo F,García-Villegas R

33-1511 was used in western blot to investigate the role of TRPV4 in a corneal epithelial cell model

Sat Jul 01 00:00:00 EDT 2017

The impact of lactoferrin with different levels of metal saturation on the intestinal epithelial barrier function and mucosal inflammation.

Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine

Majka G,Więcek G,Śróttek M,Śpiewak K,Brindell M,Koziel J,Marcinkiewicz J,Strus M

331511 was used in immunohistochemistry to study how iron-depleted, iron-saturated, and manganese-saturated forms of lactoferrin govern intestinal barrier function

Thu Dec 01 00:00:00 EST 2016

Expression of claudins -2 and -4 and cingulin is coordinated with the start of stratification and differentiation in corneal epithelial cells: retinoic acid reversibly disrupts epithelial barrier.

Biology open

Ortiz-Melo MT,Sánchez-Guzmán E,González-Robles A,Valdés J,Gómez-Flores E,Castro-Muñozledo F

Published figure using Occludin monoclonal antibody (Product # 33-1511) in Immunofluorescence

Fri Feb 15 00:00:00 EST 2013

Identification of PKCzetaII: an endogenous inhibitor of cell polarity.

The EMBO journal

Parkinson SJ,Le Good JA,Whelan RD,Whitehead P,Parker PJ

Wed Jan 14 00:00:00 EST 2004

Identification of PKCzetaII: an endogenous inhibitor of cell polarity.

The EMBO journal

Parkinson SJ,Le Good JA,Whelan RD,Whitehead P,Parker PJ

Wed Jan 14 00:00:00 EST 2004

An in vitro airway wall model of remodeling.

American journal of physiology. Lung cellular and molecular physiology

Choe MM,Sporn PH,Swartz MA

Fri Aug 01 00:00:00 EDT 2003

Role of megalin (gp330) in transcytosis of thyroglobulin by thyroid cells. A novel function in the control of thyroid hormone release.

The Journal of biological chemistry

Marinò M,Zheng G,Chiovato L,Pinchera A,Brown D,Andrews D,McCluskey RT

Fri Mar 10 00:00:00 EST 2000

Bioinformatics

Protein Aliases: Occludin; phosphatase 1, regulatory subunit 115; tight junction protein occludin; tight junction structural protein

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Gene Aliases: AI503564; BLCPMG; Ocl; OCLN; PPP1R115

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UniProt ID: (Dog) Q28269, (Human) Q16625, (Mouse) Q61146, (Rat) Q6P6T5

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Entrez Gene ID: (Dog) 403844, (Human) 100506658, (Mouse) 18260, (Rat) 83497

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Function(s)

protein binding thiopurine S-methyltransferase activity protein domain specific binding S-adenosylmethionine-dependent methyltransferase activity structural molecule activity general transcription factor

Process(es)

positive regulation of lamellipodium assembly positive regulation of microtubule polymerization bicellular tight junction assembly positive regulation of wound healing protein localization to cell leading edge protein complex assembly methylation cell-cell junction organization S-adenosylhomocysteine metabolic process S-adenosylmethionine metabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Occludin Monoclonal Antibody (OC-3F10), FITC

Occludin Antibody (33-1511) in ICC/IF

Product Details

33-1511

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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