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Occludin Antibody (740006MP647) in ICC/IF
CRISPR-Cas9 mediated genome editing of Occludin (as confirmed by next generation sequencing) was achieved by using LentiArray™ Lentiviral sgRNA (Product # A32042, AssayID CRISPR777079_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Immunofluorescence analysis was performed on wild type Caco-2 cells (panel a-c) and Caco-2 Cas9 cell line transduced with Occludin Lentiviral sgRNA cells (panel d-f). Cells were fixed, permeabilized, and labelled with Occludin Recombinant Mouse Monoclonal Antibody (OC-3F10), Alexa Fluor™ Plus 647 (Product... View More
Product Details
740006MP647
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
OC-3F10
Immunogen
GST fusion protein consisting of the C-terminal region (~150aa) of human occludin.
Conjugate
Excitation/Emission Max
658/675 nm
View spectra
Form
Liquid
Concentration
1.0 mg/mL
Purification
Protein A/G
Storage buffer
proprietary buffer, pH 6.5
Contains
0.008% Bromonitrodioxane, 0.008% Methylisothiazolone
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Wet ice
RRID
AB_3093161
Product Specific Information
Alexa Fluor™ Plus recombinant antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro.
Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.
Applications Tested: This OC-3F10 antibody has been tested by immunocytochemistry and flow cytometric analysis of Caco-2 cells. This may be used for immunocytochemistry at 0.5 µg/mL and for flow cytometry at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 658 nm; Emission: 675 nm; Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Occludin is a 65 kDa protein that can exist in a variety of phosphorylated forms, ranging up to approximately 82 kDa. Occludin is thought to be involved in regulating both the localization and the function of occludin. Polyunsaturated fatty acids are known to up-regulate occludin expression, increasing the transendothelial cell resistance and reducing the cellular permeability to large molecules. The level of occludin varies greatly depending on tissue; in brain tissue, occludin is highly and continuously expressed at cell-cell contact sites, whereas non-neural tissues show lower expression and discontinuous distribution. Overall structural features of the occludin protein are highly conserved in all the species examined. Under-expression of tight junction proteins, including occludin, is a key molecular abnormality responsible for the increased permeability of tumor endothelial tight junctions, which contributes to brain tumor edemas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Occludin; phosphatase 1, regulatory subunit 115; tight junction protein occludin
Gene Aliases: BLCPMG; OCLN; PPP1R115
UniProt ID: (Human) Q16625
Entrez Gene ID: (Human) 100506658
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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