Osenses

Olig2 Polyclonal Antibody

Olig2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Olig2 Antibody (OSO00005W-100UL) in IHC (P)

IHC-P on paraffin sections of mouse brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 mL 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chro... View More

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Product Details

OSO00005W-100UL

Applications

Tested Dilution

Publications

Western Blot (WB)

1:250

Immunohistochemistry (Paraffin) (IHC (P))

1:250

Flow Cytometry (Flow)

1:250

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Published species

Not Applicable

Host/Isotype

Rabbit / Ig

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide from human Olig2 conjugated to blue carrier protein was used as the antigen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

Conc. Not Determined

Storage buffer

whole serum

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability.

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.

The peptide is homologous in rat and mouse.

Specificity of this antibody: Olig2.

Target Information

This gene encodes a basic helix-loop-helix transcription factor which is expressed in oligodendroglial tumors of the brain. The protein is an essential regulator of ventral neuroectodermal progenitor cell fate. The gene is involved in a chromosomal translocation t(14;21)(q11.2;q22) associated with T-cell acute lymphoblastic leukemia. Its chromosomal location is within a region of chromosome 21 which has been suggested to play a role in learning deficits associated with Down syndrome. Tissue specificity: Expressed in the brain, in oligodendrocytes. Strongly expressed in oligodendrogliomas, while expression is weak to moderate in astrocytomas. Expression in glioblastomas highly variable.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Complete sequencing and characterization of 21,243 full-length human cDNAs.

Nature genetics

Ota T,Suzuki Y,Nishikawa T,Otsuki T,Sugiyama T,Irie R,Wakamatsu A,Hayashi K,Sato H,Nagai K,Kimura K,Makita H,Sekine M,Obayashi M,Nishi T,Shibahara T,Tanaka T,Ishii S,Yamamoto J,Saito K,Kawai Y,Isono Y,Nakamura Y,Nagahari K,Murakami K,Yasuda T,Iwayanagi T,Wagatsuma M,Shiratori A,Sudo H,Hosoiri T,Kaku Y,Kodaira H,Kondo H,Sugawara M,Takahashi M,Kanda K,Yokoi T,Furuya T,Kikkawa E,Omura Y,Abe K,Kamihara K,Katsuta N,Sato K,Tanikawa M,Yamazaki M,Ninomiya K,Ishibashi T,Yamashita H,Murakawa K,Fujimori K,Tanai H,Kimata M,Watanabe M,Hiraoka S,Chiba Y,Ishida S,Ono Y,Takiguchi S,Watanabe S,Yosida M,Hotuta T,Kusano J,Kanehori K,Takahashi-Fujii A,Hara H,Tanase TO,Nomura Y,Togiya S,Komai F,Hara R,Takeuchi K,Arita M,Imose N,Musashino K,Yuuki H,Oshima A,Sasaki N,Aotsuka S,Yoshikawa Y,Matsunawa H,Ichihara T,Shiohata N,Sano S,Moriya S,Momiyama H,Satoh N,Takami S,Terashima Y,Suzuki O,Nakagawa S,Senoh A,Mizoguchi H,Goto Y,Shimizu F,Wakebe H,Hishigaki H,Watanabe T,Sugiyama A,Takemoto M,Kawakami B,Yamazaki M,Watanabe K,Kumagai A,Itakura S,Fukuzumi Y,Fujimori Y,Komiyama M,Tashiro H,Tanigami A,Fujiwara T,Ono T,Yamada K,Fujii Y,Ozaki K,Hirao M,Ohmori Y,Kawabata A,Hikiji T,Kobatake N,Inagaki H,Ikema Y,Okamoto S,Okitani R,Kawakami T,Noguchi S,Itoh T,Shigeta K,Senba T,Matsumura K,Nakajima Y,Mizuno T,Morinaga M,Sasaki M,Togashi T,Oyama M,Hata H,Watanabe M,Komatsu T,Mizushima-Sugano J,Satoh T,Shirai Y,Takahashi Y,Nakagawa K,Okumura K,Nagase T,Nomura N,Kikuchi H,Masuho Y,Yamashita R,Nakai K,Yada T,Nakamura Y,Ohara O,Isogai T,Sugano S

Thu Jan 01 00:00:00 EST 2004

Bioinformatics

Protein Aliases: basic domain, helix-loop-helix protein, class B, 1; Basic helix loop helix protein class B1 (bHLHB1); basic helix-loop-helix protein 19 (bHLHe19); bHLHb1; bHLHe19; Class B basic helix-loop-helix protein 1; Class E basic helix-loop-helix protein 19; human protein kinase C-binding protein RACK17; olig-2; Oligo2; Oligo2 antibody; Oligodendrocyte specific bHLH transcription factor 2; Oligodendrocyte transcription factor 2; oligodendrocyte-specific bHLH transcription factor 2; Protein kinase C-binding protein 2; Protein kinase C-binding protein 2 (PRKCBP2); Protein kinase C-binding protein RACK17

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Gene Aliases: AI604895; BHLHB1; BHLHE19; Olg-2; OLIG2; OLIGO2; PRKCBP2; RACK17; RK17

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UniProt ID: (Human) Q13516, (Mouse) Q9EQW6

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Entrez Gene ID: (Human) 10215, (Mouse) 50913, (Rat) 304103

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Function(s)

DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding protein homodimerization activity HMG box domain binding transcription factor activity, sequence-specific DNA binding protein binding protein dimerization activity basic helix-loop-helix transcription factor

Process(es)

negative regulation of transcription from RNA polymerase II promoter transcription, DNA-templated spinal cord motor neuron differentiation spinal cord oligodendrocyte cell fate specification thalamus development myelination negative regulation of neuron differentiation neuron fate commitment positive regulation of oligodendrocyte differentiation regulation of transcription, DNA-templated transcription from RNA polymerase II promoter multicellular organism development nervous system development spinal cord oligodendrocyte cell differentiation oligodendrocyte differentiation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Olig2 Polyclonal Antibody

Olig2 Antibody (OSO00005W-100UL) in IHC (P)

Product Details

OSO00005W-100UL

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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