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Oncostatin M Antibody (500-P30BT-25UG) in ELISA
Sandwich ELISA: To detect hOncostatin M by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of Oncostatin M Polyclonal Antibody, Biotin (Product # 500-P30BT-1MG) is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Oncostatin M Polyclonal Antibody (Product # 500-P30-1MG) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hOncostatin M.
Product Details
500-P30BT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived, 25.7 kDa Recombinant Human Oncostatin M
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2930327
Product Specific Information
AA Sequence of recombinant protein: AAIGSCSKEY RVLLGQLQKQ TDLMQDTSRL LDPYIRIQGL DVPKLREHCR ERPGAFPSEE TLRGLGRRGF LQTLNATLGC VLHRLADLEQ RLPKAQDLER SGLNIEDLEK LQMARPNILG LRNNIYCMAQ LLDNSDTAEP TKAGRGASQP PTPTPASDAF QRKLEGCRFL HGYHRFMHSV GRVFSKWGES PNRSRRHSPH QALRKGVRRT RPSRKGKRLM TRGQLPR.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human Oncostatin M. Anti-Human Oncostatin M-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect hOncostatin M by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human Oncostatin M (500-P30) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hOncostatin M.
Western Blot: To detect hOncostatin M by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hOncostatin M is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P30BT-1MG will be provided as 2 x 500 µg
Target Information
Oncostatin M (OSM) is a growth and differentiation factor that participates in the regulation of neurogenesis, osteogenesis and hematopoiesis. Produced by activated T cells, monocytes and Kaposi's sarcoma cells, OSM can exert both stimulatory and inhibitory effects on cell proliferation. It stimulates the proliferation of fibroblasts, smooth muscle cells and Kaposi's sarcoma cells, but inhibits the growth of some normal and tumor cell lines. It also promotes cytokine release (e.g. IL-6, GM-CSF and G-CSF) from endothelial cells, and enhances the expression of low-density lipoprotein receptors in hepatoma cells. OSM shares several structural and functional characteristics with LIF, IL-6, and CNTF.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: MGC20461; Oncostatin-M; OSM
Gene Aliases: OSM
UniProt ID: (Human) P13725
Entrez Gene ID: (Human) 5008
positive regulation of acute inflammatory response
immune response
multicellular organism development
peripheral nervous system development
cell proliferation
positive regulation of cell proliferation
negative regulation of cell proliferation
response to heat
positive regulation of peptidyl-serine phosphorylation
oncostatin-M-mediated signaling pathway
regulation of growth
tyrosine phosphorylation of Stat3 protein
tyrosine phosphorylation of Stat5 protein
tyrosine phosphorylation of Stat1 protein
positive regulation of tyrosine phosphorylation of Stat3 protein
positive regulation of MAPK cascade
negative regulation of meiotic nuclear division
positive regulation of transcription from RNA polymerase II promoter
negative regulation of hormone secretion
behavioral response to pain
positive regulation of peptidyl-tyrosine phosphorylation
positive regulation of cell division
positive regulation of apoptotic signaling pathway
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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