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Invitrogen
Optineurin Recombinant Superclonal Antibody
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 18
Optineurin Antibody (711879) in ICC/IF
Immunofluorescence of optineurin was performed using U2OS WT and OPTN KO cells that were labeled with a green or a far-red fluorescent dye, respectively. Post-labeling, WT and KO cells were mixed and plated to a 1:1 ratio in a 96-well plate with an optically clear flat bottom as a mosaic and incubated for 24 hrs. Cells were fixed in 4% PFA (in PBS) for 15 min at RT; cells were permeabilized with 0.1% Triton... View More
Product Details
711879
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Immunogen
Protein corresponding to human Optineurin [1) aa26-aa103 2) aa416-aa510]
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2723433
Product Specific Information
This antibody is predicted to react with Monkey, Horse, Cat.
Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.
Target Information
This gene encodes the coiled-coil containing protein optineurin. Optineurin may play a role in normal-tension glaucoma and adult-onset primary open angle glaucoma. Optineurin interacts with adenovirus E3-14. 7K protein and may utilize tumor necrosis factor-alpha or Fas-ligand pathways to mediate apoptosis, inflammation or vasoconstriction. Optineurin may also function in cellular morphogenesis and membrane trafficking, vesicle trafficking, and transcription activation through its interactions with the RAB8, huntingtin, and transcription factor IIIA proteins. Alternative splicing results in multiple transcript variants encoding the same protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 14.7K-interacting protein 2; Ag9 C5; E3-14.7K-interacting protein; FIP 2; FIP-2; FIP-2-like protein; HIP 7; HIP-7; Huntingtin interacting protein L; Huntingtin yeast partner L; Huntingtin-interacting protein 7; Huntingtin-interacting protein L; NEMO-related protein; Optic neuropathy-inducing protein; Optineurin; TFIIIA IntP; TFIIIA-INTP; Transcription factor IIIA-interacting protein; transcrption factor IIIA-interacting protein; tumor necrosis factor alpha-inducible cellular protein containing leucine zipper domains; Uban
Gene Aliases: 4930441O07Rik; ALS12; FIP2; GLC1E; HIP7; HYPL; NRP; OPTN; TFIIIA-INTP
UniProt ID: (Human) Q96CV9, (Rat) Q8R5M4, (Mouse) Q8K3K8
Entrez Gene ID: (Human) 10133, (Rat) 246294, (Mouse) 71648
protein targeting to Golgi
G2/M transition of mitotic cell cycle
negative regulation of receptor recycling
Golgi organization
signal transduction
cell death
macroautophagy
Golgi to plasma membrane protein transport
regulation of I-kappaB kinase/NF-kappaB signaling
negative regulation of I-kappaB kinase/NF-kappaB signaling
defense response to Gram-negative bacterium
parkin-mediated mitophagy in response to mitochondrial depolarization
Golgi ribbon formation
xenophagy
autophagy
positive regulation of cell proliferation
cellular protein localization
wound healing
negative regulation of neuron apoptotic process
cytokine secretion
protein homooligomerization
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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