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Osteocalcin Antibody (PA5-78871) in IHC (P)
Immunohistochemistry analysis of Osteocalcin on paraffin-embedded rat tibia tissue. Antigen retrieval was performed using citrate buffer (pH6, epitope retrieval solution) for 20 mins. Sample was blocked using 10% goat serum, incubated with Osteocalcin polyclonal antibody (Product# PA5-78871) with a dilution of 1 µg/mL (overnight at 4°C), and followed by biotinylated goat anti-rabbit IgG (30 minutes at 37°C)... View More
Product Details
PA5-78871
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Rat
Published species
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived rat Osteocalcin recombinant protein (Position: Y50-V99).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2745987
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Target Information
Osteocalcin belongs to the osteocalcin/matrix Gla-protein family and constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium. Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: BGP; bone gamma-carboxyglutamate (gla) protein (osteocalcin); bone gamma-carboxyglutamate protein 2; bone gamma-carboxyglutamic acid (Gla) protein (osteocalcin); Bone Gla protein; bone Gla-protein; Gamma-carboxyglutamic acid-containing protein; Osteocalcin; PMF1; RP11-54H19.5
Gene Aliases: Bglap; Bglap2; Bgp; Bgpr; Bgpra
UniProt ID: (Rat) P04640
Entrez Gene ID: (Rat) 25295
ossification
osteoblast differentiation
osteoblast development
aging
cell aging
response to mechanical stimulus
response to gravity
response to inorganic substance
response to zinc ion
response to organic cyclic compound
response to activity
regulation of bone mineralization
biomineral tissue development
response to nutrient levels
response to vitamin K
response to vitamin D
response to testosterone
response to hydroxyisoflavone
odontogenesis
response to drug
response to estrogen
response to ethanol
response to glucocorticoid
bone development
cellular response to vitamin D
cellular response to growth factor stimulus
cellular response to BMP stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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