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Invitrogen
Osteocalcin Recombinant Rabbit Monoclonal Antibody (2T1J7)
FIGURE: 1 / 2
Osteocalcin Antibody (MA5-51225) in WB
Western blot analysis of Osteocalcin in extracts of various cell lines. Samples were incubated with Osteocalcin Recombinant monoclonal antibody (Product # MA5-51225) using a dilution of 1:1,000 followed by HRP Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Product Details
MA5-51225
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
2T1J7
Immunogen
Recombinant protein of Human BGLAP.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_3094199
Target Information
Osteocalcin belongs to the osteocalcin/matrix Gla-protein family and constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium. Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: BGP; bone gamma carboxyglutamate protein 1; bone gamma-carboxyglutamate (gla) protein (osteocalcin); bone gamma-carboxyglutamate protein 2; bone gamma-carboxyglutamic acid (Gla) protein (osteocalcin); Bone Gla protein; bone Gla-protein; Gamma-carboxyglutamic acid-containing protein; OG1; Osteocalcin; PMF1; RP11-54H19.5
Gene Aliases: BGLAP; Bglap1; Bglap2; BGP; Bgpr; Bgpra; mOC-A; OC; OCN; OG1
UniProt ID: (Human) P02818, (Mouse) P86546, (Rat) P04640
Entrez Gene ID: (Human) 632, (Mouse) 12096, (Rat) 25295
regulation of bone mineralization
biomineral tissue development
cellular response to BMP stimulus
skeletal system development
osteoblast differentiation
osteoblast development
signal peptide processing
ER to Golgi vesicle-mediated transport
cell adhesion
cell aging
response to mechanical stimulus
response to gravity
response to zinc ion
response to activity
peptidyl-glutamic acid carboxylation
bone mineralization
response to vitamin K
response to vitamin D
response to testosterone
response to hydroxyisoflavone
odontogenesis
response to drug
response to estrogen
regulation of bone resorption
response to ethanol
regulation of osteoclast differentiation
response to glucocorticoid
bone development
cellular response to vitamin D
cellular response to growth factor stimulus
ossification
aging
response to inorganic substance
response to organic cyclic compound
response to nutrient levels
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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