Proteintech

Osteopontin Polyclonal Antibody

Osteopontin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Osteopontin Antibody (25715-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded human small intestine tissue slide using 25715-1-AP (Osteopontin antibody at dilution of 1:200 (under 40x lens).

Osteopontin Antibody in Western Blot (WB)

Product Image Gallery

Product Details

25715-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 15 publications 15 publications

Immunohistochemistry (IHC)

View 7 publications 7 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Osteopontin Fusion Protein Ag22588 (1-300 aa encoded by BC007016)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.28 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MRIAVICFC LLGITCAIPV KQADSGSSEE KQLYNKYPDA VATWLNPDPS QKQNLLAPQT LPSKSNESHD HMDDMDDEDD DDHVDSQDSI DSNDSDDVDD TDDSHQSDES HHSDESDELV TDFPTDLPAT EVFTPVVPTV DTYDGRGDSV VYGLRSKSKK FRRPDIQYPD ATDEDITSHM ESEELNGAYK AIPVAQDLNA PSDWDSRGKD SYETSQLDDQ SAETHSHKQS RLYKRKANDE SNEHSDVIDS QELSKVSREF HSHEFHSHED MLVVDPKSKE EDKHLKFRIS HELDSASSEV N (1-300 aa encoded by BC007016)

Target Information

Osteopontin is an arginine-glycine-aspartic acid (RGD)-containing glycoprotein that interacts with integrins and CD44 as major receptors. It is a multifunctional protein involved in bone mineralization, cell adhesion, cell migration, chronic inflammatory disease and transformation. Proteolytic cleavage by thrombin and matrix metalloproteinases close to the integrin-binding Arg-Gly-Asp sequence modulates the function of OPN and its integrin binding properties. Thrombin-cleaved fragments of Osteopontin are overexpressed in malignant glial tumors and provide a molecular niche with survival advantage and provide a novel substrate for plasmin and cathepsin D.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Tax1 binding protein 3 regulates osteogenic and adipogenic differentiation through inactivating Wnt/β-catenin signalling.

Journal of cellular and molecular medicine

Wang Y,Gan Y,Dong Y,Zhou J,Zhu E,Yuan H,Li X,Wang B

Sat Apr 01 00:00:00 EDT 2023

Myocardin Reverses Hypoxia-Inducible Factor-1α Mediated Phenotypic Modulation of Corpus Cavernosum Smooth Muscle Cells in Hypoxia Induced by Cobalt Chloride.

The world journal of men's health

Zhou X,Luo C,Fan J,Gao G,Wang T,Zhang H,Wei A

Sat Apr 01 00:00:00 EDT 2023

miRNA‑92a inhibits vascular smooth muscle cell phenotypic modulation and may help prevent in‑stent restenosis.

Molecular medicine reports

Jiang F,Zhang B,Zhang X,Zhang R,Lu Q,Shi F,Xu J,Deng L

Wed Feb 01 00:00:00 EST 2023

Contribution of calcium dysregulation to impaired coronary artery contraction in Zucker diabetic fatty rats.

Clinical and experimental pharmacology & physiology

Liu L,Yang H,Kuang SJ,Zhang L,Zhou MY,Zeng P,Zhang MZ,Rao F,Zhou ZL,Deng CY

Wed Feb 01 00:00:00 EST 2023

Proteomics and transcriptomics profiling reveals distinct aspects of kidney stone related genes in calculi rats.

BMC genomics

Zhu W,Qiong D,Yanli G,Min L,Ying Z,Qiyi H,Shenping Z,Xisheng W,Hui L

Fri Mar 17 00:00:00 EDT 2023

Human Amniotic Membrane Promotes Angiogenesis in an Oxidative Stress Chronic Diabetic Murine Wound Model.

Advances in wound care

Wu M,Yu Z,Matar DY,Karvar M,Chen Z,Ng B,Aoki S,Haug V,Orgill DP,Panayi AC

Thu Jun 01 00:00:00 EDT 2023

M1 macrophage-derived extracellular vesicle containing tsRNA-5006c promotes osteogenic differentiation of aortic valve interstitial cells through regulating mitophagy.

PeerJ

Xia H,Gao M,Chen J,Huang G,Xiang X,Wang Y,Huang Z,Li Y,Su S,Zhao Z,Zeng Q,Ruan Y

Mon Jan 23 00:00:00 EST 2023

Aryl hydrocarbon receptor activation-mediated vascular toxicity of ambient fine particulate matter: contribution of polycyclic aromatic hydrocarbons and osteopontin as a biomarker.

Particle and fibre toxicology

Ho CC,Wu WT,Lin YJ,Weng CY,Tsai MH,Tsai HT,Chen YC,Yet SF,Lin P

Thu Jun 23 00:00:00 EDT 2022

Deficient DNASE1L3 facilitates neutrophil extracellular traps-induced invasion via cyclic GMP-AMP synthase and the non-canonical NF-κB pathway in diabetic hepatocellular carcinoma.

Clinical & translational immunology

Li N,Zheng X,Chen M,Huang L,Chen L,Huo R,Li X,Huang Y,Sun M,Mai S,Wu Z,Zhang H,Liu J,Yang CT

Fri Apr 29 00:00:00 EDT 2022

Metastasis suppressor 1 controls osteoblast differentiation and bone homeostasis through regulating Src-Wnt/β-catenin signaling.

Cellular and molecular life sciences : CMLS

Chen M,Shan L,Gan Y,Tian L,Zhou J,Zhu E,Yuan H,Li X,Wang B

Sun Jan 30 00:00:00 EST 2022

Citrate regulates extracellular matrix mineralization during osteoblast differentiation in vitro.

Journal of inorganic biochemistry

Wu X,Dai H,Yu S,Zhao Y,Long Y,Li W,Tu J

Fri Jan 01 00:00:00 EST 2021

A CREB1-miR-181a-5p loop regulates the pathophysiologic features of bone marrow stromal cells in fibrous dysplasia of bone.

Molecular medicine (Cambridge, Mass.)

Fu Y,Xin Z,Ling Z,Xie H,Xiao T,Shen X,Lin J,Xu L,Jiang H

Thu Jul 22 00:00:00 EDT 2021

The Preventive Effect of Systemic Honokiol and Systemic Pentoxifylline on Epidural Fibrosis.

Turkish neurosurgery

Secinti KD,Secinti IE,Kurutas EB

Thu Jan 13 00:00:00 EST 2022

Diversified transcriptional responses of myeloid and glial cells in spinal cord injury shaped by HDAC3 activity.

Science advances

Wahane S,Zhou X,Zhou X,Guo L,Friedl MS,Kluge M,Ramakrishnan A,Shen L,Friedel CC,Zhang B,Friedel RH,Zou H

Mon Feb 01 00:00:00 EST 2021

High-mobility group AT-Hook 1 mediates the role of nuclear factor I/X in osteogenic differentiation through activating canonical Wnt signaling.

Stem cells (Dayton, Ohio)

Wu X,Wang X,Shan L,Zhou J,Zhang X,Zhu E,Yuan H,Wang B

Fri Oct 01 00:00:00 EDT 2021

Microglia and macrophages promote corralling, wound compaction and recovery after spinal cord injury via Plexin-B2.

Nature neuroscience

Zhou X,Wahane S,Friedl MS,Kluge M,Friedel CC,Avrampou K,Zachariou V,Guo L,Zhang B,He X,Friedel RH,Zou H

Sun Mar 01 00:00:00 EST 2020

Magnesium Calcium Phosphate Cement Incorporating Citrate for Vascularized Bone Regeneration.

ACS biomaterials science & engineering

Wu X,Dai H,Yu S,Zhao Y,Long Y,Li W,Tu J

Mon Nov 09 00:00:00 EST 2020

Magnesium Calcium Phosphate Cement Incorporating Citrate for Vascularized Bone Regeneration.

ACS biomaterials science & engineering

Wu X,Dai H,Yu S,Zhao Y,Long Y,Li W,Tu J

Mon Nov 09 00:00:00 EST 2020

Strong Signs for a Weak Wall in Tricuspid Aortic Valve Associated Aneurysms and a Role for Osteopontin in Bicuspid Aortic Valve Associated Aneurysms.

International journal of molecular sciences

Stern C,Scharinger B,Tuerkcan A,Nebert C,Mimler T,Baranyi U,Doppler C,Aschacher T,Andreas M,Stelzmueller ME,Ehrlich M,Graf A,Laufer G,Bernhard D,Messner B

Thu Sep 26 00:00:00 EDT 2019

Gene signatures of quiescent glioblastoma cells reveal mesenchymal shift and interactions with niche microenvironment.

EBioMedicine

Tejero R,Huang Y,Katsyv I,Kluge M,Lin JY,Tome-Garcia J,Daviaud N,Wang Y,Zhang B,Tsankova NM,Friedel CC,Zou H,Friedel RH

Mon Apr 01 00:00:00 EDT 2019

Gene signatures of quiescent glioblastoma cells reveal mesenchymal shift and interactions with niche microenvironment.

EBioMedicine

Tejero R,Huang Y,Katsyv I,Kluge M,Lin JY,Tome-Garcia J,Daviaud N,Wang Y,Zhang B,Tsankova NM,Friedel CC,Zou H,Friedel RH

Mon Apr 01 00:00:00 EDT 2019

Effects of nicotine on the metabolism and gene expression profile of Sprague‑Dawley rat primary osteoblasts.

Molecular medicine reports

Liang D,Wang KJ,Tang ZQ,Liu RH,Zeng F,Cheng MY,Lian QS,Wu HK

Fri Jun 01 00:00:00 EDT 2018

Resveratrol Ameliorated Vascular Calcification by Regulating Sirt-1 and Nrf2.

Transplantation proceedings

Zhang P,Li Y,Du Y,Li G,Wang L,Zhou F

Thu Dec 01 00:00:00 EST 2016

Resveratrol Ameliorated Vascular Calcification by Regulating Sirt-1 and Nrf2.

Transplantation proceedings

Zhang P,Li Y,Du Y,Li G,Wang L,Zhou F

Thu Dec 01 00:00:00 EST 2016

Bioinformatics

Protein Aliases: 2AR; 44 kDa bone phosphoprotein; Bone sialoprotein 1; Bone sialoprotein 1 (BSPI/BSP1); Calcium oxalate crystal growth inhibitor protein; CALPHA1 fusion; Early T lymphocyte activation 1 (ETA1); early T-lymphocyte activation 1; Early T-lymphocyte activation 1 protein; HGNC:11255; immunoglobulin alpha 1 heavy chain constant region fusion protein; MGC110940; Minopontin; Nephropontin; Osteopontin; osteopontin-like protein; osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein; Secreted phosphoprotein 1; secreted phosphoprotein 1 (osteopontin bone sialoprotein I early T lymphocyte activation 1); secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1); Secreted phosphoprotein 1 (SPP1); secreted phosphoprotein 1 variant 6; Sialoprotein (osteopontin); SPP-1; SPP1/CALPHA1 fusion; urinar; Urinary stone protein; Uropontin

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Gene Aliases: 2AR; 2b7; Apl-1; BNSP; Bsp; BSPI; Eta; ETA-1; OP; OPN; Opnl; OSP; PSEC0156; Ric; Spp-1; SPP1

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UniProt ID: (Human) P10451, (Mouse) P10923, (Rat) P08721

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Entrez Gene ID: (Human) 6696, (Mouse) 20750, (Rat) 25353

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Function(s)

cytokine activity protein binding extracellular matrix binding cytokine

Process(es)

osteoblast differentiation inflammatory response cell adhesion embryo implantation extracellular matrix disassembly extracellular matrix organization biomineral tissue development response to vitamin D positive regulation of bone resorption decidualization response to steroid hormone negative regulation of collateral sprouting of intact axon in response to injury ossification response to organic substance positive regulation of cell-substrate adhesion neutrophil chemotaxis negative regulation of apoptotic process cellular response to fluid shear stress cell differentiation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Osteopontin Polyclonal Antibody

Osteopontin Antibody (25715-1-AP) in IHC (P)

Product Details

25715-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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