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Invitrogen
Osteopontin Polyclonal Antibody
FIGURE: 1 / 2
Osteopontin Antibody (PA1-25152) in ICC/IF
Oncotarget 2016 -
Figure 1 CTD-deficient cells display altered properties in their cell behaviour and phenotype ( A ) CTD-deficient mice (DeltaT) express a carboxyl-terminal truncated Cx43 (K258Stop) instead of the WT Cx43 isoform [ 32 ]. ( B and C ) Mouse genotyping was performed by PCR and confirmed by western blot using an antibody raised against the N-terminal region of Cx43, in order to detect the full length (+/+ and +... View More
Product Details
PA1-25152
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant, full length mouse protein, expressed in NSO cells.
Conjugate
Form
Liquid
Concentration
0.1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_795087
Product Specific Information
By Western Blot, a doublet band of approximately 65 kDa and a band of 30 kDa is observed due to glycosylation. A suggested positive control is 293 cells. The Neutralization Dose50 (ND50) for anti-mouse osteopontin is approximately 1 to 3 µg/mL in the presence of 2 µg/mL of recombinant mouse osteopontin using 293 cells.
Target Information
Osteopontin is an arginine-glycine-aspartic acid (RGD)-containing glycoprotein that interacts with integrins and CD44 as major receptors. It is a multifunctional protein involved in bone mineralization, cell adhesion, cell migration, chronic inflammatory disease and transformation. Proteolytic cleavage by thrombin and matrix metalloproteinases close to the integrin-binding Arg-Gly-Asp sequence modulates the function of OPN and its integrin binding properties. Thrombin-cleaved fragments of Osteopontin are overexpressed in malignant glial tumors and provide a molecular niche with survival advantage and provide a novel substrate for plasmin and cathepsin D.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 2AR; 44 kDa bone phosphoprotein; Bone sialoprotein 1; Bone sialoprotein 1 (BSPI/BSP1); Calcium oxalate crystal growth inhibitor protein; CALPHA1 fusion; Early T lymphocyte activation 1 (ETA1); Early T-lymphocyte activation 1 protein; HGNC:11255; immunoglobulin alpha 1 heavy chain constant region fusion protein; MGC110940; Minopontin; Osteopontin; osteopontin-like protein; Secreted phosphoprotein 1; secreted phosphoprotein 1 (osteopontin bone sialoprotein I early T lymphocyte activation 1); Secreted phosphoprotein 1 (SPP1); SPP-1; urinar
Gene Aliases: 2AR; Apl-1; BNSP; Bsp; BSPI; Eta; ETA-1; OP; Opn; Opnl; Ric; Spp-1; Spp1
UniProt ID: (Mouse) P10923
Entrez Gene ID: (Mouse) 20750
ossification
osteoblast differentiation
cell adhesion
response to organic substance
positive regulation of cell-substrate adhesion
neutrophil chemotaxis
biomineral tissue development
response to vitamin D
negative regulation of apoptotic process
positive regulation of bone resorption
response to steroid hormone
cellular response to fluid shear stress
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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