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Invitrogen
Osteopontin Polyclonal Antibody
FIGURE: 1 / 5
Osteopontin Antibody (PA5-13494) in IHC (P)
Immunohistochemistry analysis of Osteopontin in formalin-fixed and paraffin-embedded human lung carcinoma. Samples were incubated with Osteopontin polyclonal antibody (Product # PA5-13494) which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Product Details
PA5-13494
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Not Applicable
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide between 273-301 amino acids from the C-terminal region of human SPP1
Conjugate
Form
Liquid
Concentration
2 mg/mL
Purification
Size-exclusion - Dialysis, Ammonium sulfate precipitation
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2286594
Target Information
Osteopontin is an arginine-glycine-aspartic acid (RGD)-containing glycoprotein that interacts with integrins and CD44 as major receptors. It is a multifunctional protein involved in bone mineralization, cell adhesion, cell migration, chronic inflammatory disease and transformation. Proteolytic cleavage by thrombin and matrix metalloproteinases close to the integrin-binding Arg-Gly-Asp sequence modulates the function of OPN and its integrin binding properties. Thrombin-cleaved fragments of Osteopontin are overexpressed in malignant glial tumors and provide a molecular niche with survival advantage and provide a novel substrate for plasmin and cathepsin D.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Bone sialoprotein 1; Bone sialoprotein 1 (BSPI/BSP1); CALPHA1 fusion; Early T lymphocyte activation 1 (ETA1); early T-lymphocyte activation 1; HGNC:11255; immunoglobulin alpha 1 heavy chain constant region fusion protein; MGC110940; Nephropontin; Osteopontin; osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein; Secreted phosphoprotein 1; secreted phosphoprotein 1 (osteopontin bone sialoprotein I early T lymphocyte activation 1); secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1); Secreted phosphoprotein 1 (SPP1); secreted phosphoprotein 1 variant 6; SPP-1; SPP1/CALPHA1 fusion; urinar; Urinary stone protein; Uropontin
Gene Aliases: BNSP; BSPI; ETA-1; OPN; PSEC0156; SPP1
UniProt ID: (Human) P10451
Entrez Gene ID: (Human) 6696
osteoblast differentiation
inflammatory response
cell adhesion
embryo implantation
extracellular matrix disassembly
extracellular matrix organization
biomineral tissue development
response to vitamin D
positive regulation of bone resorption
decidualization
response to steroid hormone
negative regulation of collateral sprouting of intact axon in response to injury
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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