Invitrogen

Osteopontin Monoclonal Antibody (7C5H12)

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Antibody Testing Data (4)
Published Figures (5)

Osteopontin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 9

Osteopontin Antibody (MA5-17180) in IHC (P)

Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues using SPP1 monoclonal antibody (Product # MA5-17180) followed with DAB staining.

Osteopontin Antibody in Western Blot (WB)

Osteopontin Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Product Details

MA5-17180

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 8 publications 8 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:1,000

Immunocytochemistry (ICC/IF)

1:200-1:1,000

View 3 publications 3 publications

ELISA (ELISA)

1:10,000

Product Specifications

Species Reactivity

Human

Published species

Human, Rabbit, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

7C5H12

Immunogen

Purified recombinant fragment of human SPP1 (amino acids: 167-314) expressed in E. Coli.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2538651

Product Specific Information

MA5-17180 targets SPP1 in indirect ELISA, ICC, IHC, IF and WB applications and shows reactivity with Human samples.

The MA5-17180 immunogen is purified recombinant fragment of human SPP1 (amino acids: 167-314) expressed in E. Coli.

MA5-17180 detects SPP1 which has a predicted molecular weight of approximately 35.4kDa.

Target Information

Osteopontin is an arginine-glycine-aspartic acid (RGD)-containing glycoprotein that interacts with integrins and CD44 as major receptors. It is a multifunctional protein involved in bone mineralization, cell adhesion, cell migration, chronic inflammatory disease and transformation. Proteolytic cleavage by thrombin and matrix metalloproteinases close to the integrin-binding Arg-Gly-Asp sequence modulates the function of OPN and its integrin binding properties. Thrombin-cleaved fragments of Osteopontin are overexpressed in malignant glial tumors and provide a molecular niche with survival advantage and provide a novel substrate for plasmin and cathepsin D.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A serum protein signature at the time of Uveal Melanoma diagnosis predicts long-term patient survival.

BMC cancer

Herrspiegel C,Plastino F,Lardner E,Seregard S,Williams PA,André H,Stålhammar G

MA5-17180 was used in Immunohistochemistry to develop a prognostic test (serUM-Px) for uveal melanoma patients based on peripheral serum samples obtained at the time of diagnosis, which stratifies them into low, intermediate, and high metastatic risk categories, with low serum leptin levels and high osteopontin levels as key biomarkers, showing promise as an independent predictor of metastasis.

Mon Mar 27 00:00:00 EDT 2023

A serum protein signature at the time of Uveal Melanoma diagnosis predicts long-term patient survival.

BMC cancer

Herrspiegel C,Plastino F,Lardner E,Seregard S,Williams PA,André H,Stålhammar G

Mon Mar 27 00:00:00 EDT 2023

Expression of Extracellular Matrix-Related Genes and Their Regulatory microRNAs in Problematic Colorectal Polyps.

Cancers

Žlajpah M,Boštjančič E,Tepeš B,Zidar N

Published figure using Osteopontin monoclonal antibody (Product # MA5-17180) in Immunohistochemistry

Fri Dec 11 00:00:00 EST 2020

Expression of Extracellular Matrix-Related Genes and Their Regulatory microRNAs in Problematic Colorectal Polyps.

Cancers

Žlajpah M,Boštjančič E,Tepeš B,Zidar N

Published figure using Osteopontin monoclonal antibody (Product # MA5-17180) in Immunohistochemistry

Fri Dec 11 00:00:00 EST 2020

The effect of graft application and simvastatin treatment on tibial bone defect in rats. A histological and immunohistochemical study.

Acta cirurgica brasileira

Laçin N,İzol BS,Özkorkmaz EG,Deveci B,Tuncer MC

MA5-17180 was used in Immunohistochemistry to establish the increasing effect of simvastatin in bone turnover in rats, by increasing osteoinductive effect with graft.

Mon May 06 00:00:00 EDT 2019

The effect of graft application and allopurinol treatment on calvarial bone defect in rats1.

Acta cirurgica brasileira

Laçin N,İzol BS,Özkorkmaz EG,Deveci B,Tuncer MC

MA5-17180 was used in Immunohistochemistry to investigate the effects of allopurinol administration on osteoinductive reaction and bone development with graft material.

Mon Mar 18 00:00:00 EDT 2019

Short-term use of resveratrol in alloplastic graft material applied with calvarial bone defects in rats.

Acta cirurgica brasileira

Laçin N,Deveci E

MA5-17180 was used in Immunohistochemistry to show that new bone formation in the calvaral defect region of rats can be induced by short term treatment of resveratrol in alloplastic graft material.

Thu Sep 12 00:00:00 EDT 2019

Ganoderma lucidum, a promising agent possessing antioxidant and anti-inflammatory effects for treating calvarial defects with graft application in rats.

Acta cirurgica brasileira

Laçin N,İzol SB,İpek F,Tuncer MC

MA5-17180 was used in Immunohistochemistry to show that, with its antioxidant and anti-inflammatory properties, G. Lucidum is an important factor in the treatment of calvarial bone defects.

Fri Dec 13 00:00:00 EST 2019

Biochemical and immunohistochemical investigations on bone formation and remodelling in ovariectomised rats with tamoxifen citrate administration.

Folia morphologica

Baloğlu M,Gökalp Özkorkmaz E

MA5-17180 was used in Immunohistochemistry to observe that Osteonectin promotes bone formation by influencing collagen fiber formation, extracellular matrix development, osteoblast differentiation and the capacity to affect osteoclast activity.

Tue Jul 07 00:00:00 EDT 2020

Autophagy drives osteogenic differentiation of human gingival mesenchymal stem cells.

Cell communication and signaling : CCS

Vidoni C,Ferraresi A,Secomandi E,Vallino L,Gardin C,Zavan B,Mortellaro C,Isidoro C

MA5-17180 was used in Immunocytochemistry-immunoflourescence to study resveratrol-induced osteogenic differentiation of human gingival mesenchymal stem cells, and provide evidence for the essential role of autophagy modulation in this process.

Mon Aug 19 00:00:00 EDT 2019

Autophagy drives osteogenic differentiation of human gingival mesenchymal stem cells.

Cell communication and signaling : CCS

Vidoni C,Ferraresi A,Secomandi E,Vallino L,Gardin C,Zavan B,Mortellaro C,Isidoro C

Mon Aug 19 00:00:00 EDT 2019

Human adipose stem cells induced to osteogenic differentiation by an innovative collagen/hydroxylapatite hybrid scaffold.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Mazzoni E,D'Agostino A,Manfrini M,Maniero S,Puozzo A,Bassi E,Marsico S,Fortini C,Trevisiol L,Patergnani S,Tognon M

MA5-17180 was used in Immunocytochemistry-immunoflourescence to investigate human adipose stem cells for osteogenic, osteoconductivity, and osteoinductivity effects of an innovative collagen/ hydroxylapatite hybrid scaffold.

Sun Oct 01 00:00:00 EDT 2017

Regeneration of a goat femoral head using a tissue-specific, biphasic scaffold fabricated with CAD/CAM technology.

Biomaterials

Ding C,Qiao Z,Jiang W,Li H,Wei J,Zhou G,Dai K

MA5-17180 was used in immunohistochemistry to study the use of CAD/CAM technology to aid the construction of biphasic scaffolds for the tissue engineering repair of the femoral head in a goat model

Sun Sep 01 00:00:00 EDT 2013

Restoration of critical-size defects in the rabbit mandible using porous nanohydroxyapatite-polyamide scaffolds.

Tissue engineering. Part A

Guo J,Meng Z,Chen G,Xie D,Chen Y,Wang H,Tang W,Liu L,Jing W,Long J,Guo W,Tian W

MA5-17180 was used in immunocytochemistry to study the use of porous nanohydroxyapatite-polyamide-based scaffolds to aid the repair of rabbit mandible bone defects

Fri Jun 01 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: Bone sialoprotein 1; Bone sialoprotein 1 (BSPI/BSP1); CALPHA1 fusion; Early T lymphocyte activation 1 (ETA1); early T-lymphocyte activation 1; HGNC:11255; immunoglobulin alpha 1 heavy chain constant region fusion protein; MGC110940; Nephropontin; Osteopontin; osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein; Secreted phosphoprotein 1; secreted phosphoprotein 1 (osteopontin bone sialoprotein I early T lymphocyte activation 1); secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1); Secreted phosphoprotein 1 (SPP1); secreted phosphoprotein 1 variant 6; SPP-1; SPP1/CALPHA1 fusion; urinar; Urinary stone protein; Uropontin

View more View less

Gene Aliases: BNSP; BSPI; ETA-1; OPN; PSEC0156; SPP1

View more View less

UniProt ID: (Human) P10451

View more View less

Entrez Gene ID: (Human) 6696

View more View less

Function(s)

cytokine activity protein binding extracellular matrix binding cytokine

Process(es)

osteoblast differentiation inflammatory response cell adhesion embryo implantation extracellular matrix disassembly extracellular matrix organization biomineral tissue development response to vitamin D positive regulation of bone resorption decidualization response to steroid hormone negative regulation of collateral sprouting of intact axon in response to injury

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

Osteopontin Monoclonal Antibody (7C5H12)

Osteopontin Antibody (MA5-17180) in IHC (P)

Product Details

MA5-17180

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!