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Invitrogen
P-Cadherin Monoclonal Antibody (CSTEM29), eBioscience™
FIGURE: 1 / 4
P-Cadherin Antibody (14-2237-82) in ICC/IF
Immunofluorescence analysis of Cadherin-3 was performed using 70% confluent log phase A-431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.01% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with P-Cadherin Monoclonal Antibody (CSTEM29), eBioscience™ (Product # 14-2237-82) at 5µg/mL in 0.1% BSA, incub... View More
Product Details
14-2237-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Mouse
/ IgG2a, kappa
Class
Monoclonal
Type
Antibody
Clone
CSTEM29
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2688283
Product Specific Information
This monoclonal antibody CSTEM29 reacts with human P-Cadherin, also known as Cadherin-3.
Applications Reported: This CSTEM29 antibody has been reported for use in flow cytometric analysis and immunoblot.
Applications Tested: This CSTEM29 antibody has been tested by flow cytometric analysis of normal human induced pluripotent stem cells, and for western blot. For flow cytometry, this may be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. For western blot, this can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
P-cadherin, also known as Cadherin-3, is a Type 1 cadherin protein that belongs to the cadherin superfamily. Type 1 cadherins are single-pass transmembrane proteins that have 5 extracellular cadherin repeats and an intracellular domain that binds p120-catenin and beta-catenin. Cadherins are calcium-dependent cell-cell adhesion glycoproteins responsible for a range of processes including development, wound healing, cell-cell signaling, cell growth and differentiation. P-cadherin is expressed in human fetal structures, and in adult tissues such as the basal layer of the epidermis, breast, prostate, ovary, cervix, hair follicle, and corneal endothelium. P-cadherin expression has also been reported on embryonic stem cells, and stem cells of the normal mammary gland, and hair follicle. Overexpression of P-cadherin has been associated with poor prognosis in breast, prostate, ovary, colon, and stomach carcinomas. Mutations in CDH3 are associated with congential hypotrichosis with juvenile macular dystrophy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CADH3; cadherin 3, type 1, P-cadherin (placental); Cadherin-3; calcium-dependent adhesion protein, placental; P-cadherin; Placental cadherin; Placental-cadherin; RPE-specific cadherin
Gene Aliases: AI385538; Cadp; CDH3; CDHP; HJMD; P-cadherin; PCAD
UniProt ID: (Human) P22223, (Mouse) P10287
Entrez Gene ID: (Human) 1001, (Mouse) 12560
retina homeostasis
cell adhesion
homophilic cell adhesion via plasma membrane adhesion molecules
visual perception
positive regulation of gene expression
positive regulation of keratinocyte proliferation
single organismal cell-cell adhesion
hair cycle process
keratinization
positive regulation of monophenol monooxygenase activity
negative regulation of transforming growth factor beta2 production
adherens junction organization
wound healing
response to drug
positive regulation of insulin-like growth factor receptor signaling pathway
positive regulation of melanin biosynthetic process
negative regulation of catagen
canonical Wnt signaling pathway
regulation of hair cycle by canonical Wnt signaling pathway
positive regulation of melanosome transport
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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