Invitrogen

P-cadherin Monoclonal Antibody (PCD-1)

11 Published Figures

32 References

View all (30) P-cadherin antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Published Figures (11)

P-cadherin Antibody in Immunohistochemistry (IHC)

FIGURE: 1 / 11

P-cadherin Antibody (13-2000Z) in IHC

Current issues in molecular biology 2023 - Representative microphotographs of immunohistochemical staining using antibodies against E-cadherin (E-cad) (DAKO, M3612, NCH-38) ( A ), E-cadherin (ProteinTech, 20874-1-AP) ( B ), N-cadherin (N-cad) ( C ), P-cadherin (P-cad) (Santa Cruz, sc-74545) ( D ), beta-catenin ( E ), and vimentin ( F ). Insets of ( A - F ) show enlarged images of microphotographs. Images from cases 1 ( F ), 3 ( C ), 5 ( E ), 6 ( A )... View More

P-cadherin Antibody in Immunocytochemistry (ICC/IF)

P-cadherin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

P-cadherin Antibody in Western Blot, Immunohistochemistry (Paraffin) (WB, IHC (P))

P-cadherin Antibody in Immunohistochemistry (Frozen) (IHC (F))

P-cadherin Antibody in Western Blot (WB)

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Product Details

13-2000Z

Applications

Tested Dilution

Publications

Western Blot (WB)

10 µg/mL

View 3 publications 3 publications

Immunohistochemistry (IHC)

View 9 publications 9 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 4 publications 4 publications

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

View 6 publications 6 publications

Flow Cytometry (Flow)

View 2 publications 2 publications

Inhibition Assays (IA)

60 µg/mL

In vitro Assay (IV)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 4 publications 4 publications

Product Specifications

Species Reactivity

Mouse

Published species

Bovine, Fish, Hamster, Human, Mouse, Rat, Sheep, Tag

Host/Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

PCD-1

Immunogen

Mouse endoderm cell line PSA5-E.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

2.0 mg/mL

Purification

Ion-exchange chromatography

Storage buffer

PBS, pH 7.4, with 1% BSA

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533006

Product Specific Information

Source: Monoclonal antibody was obtained by fusing the P3-X63-Ag8-U1 mouse myeloma cell-line with spleen cells of Donryu rat after immunization with mouse endoderm cell line PSA5-E.

Specificity: This antibody specifically reacts with mouse P-cadherin. This antibody inhibits P-cadherin-dependent cell-cell contact.

Cross reactivity: This antibody does not react with human or chicken P-cadherin.

Storage: 4°C This product does not contain preservative. The stock solution (2.0 mg/ml) should be stored in aliquots at -20°C for 1 year, or should be stored at 4°C for 6 months after adding 0.1% sodium azide. Avoid repeated freeze-thaw cycles.

Reconstitution: Dissolve the lyophilized antibody in 50 µl of distilled water (final concentration: 2.0 mg/ml). This solution can be used as a stock solution. If dilution is necessary for your application, dilute the stock solution with the following Dilution solution just prior to use. When the entire amount of antibody is to be used over a short time period, it may be dissolved directly in 500 µl or more of the Dilution solution. Be sure to store the antibody at a minimum concentration of 2.0 mg/ml. A lower antibody concentration may result in decreased stability. Reconstituted antibody solution should contain 0.1% sodium azide as a preservative when stored at 4°C.

Ca2+ should be contained in the buffers to stabilize cadherin antigen.

Target Information

P-cadherin, also known as Cadherin-3, is a Type 1 cadherin protein that belongs to the cadherin superfamily. Type 1 cadherins are single-pass transmembrane proteins that have 5 extracellular cadherin repeats and an intracellular domain that binds p120-catenin and beta-catenin. Cadherins are calcium-dependent cell-cell adhesion glycoproteins responsible for a range of processes including development, wound healing, cell-cell signaling, cell growth and differentiation. P-cadherin is expressed in human fetal structures, and in adult tissues such as the basal layer of the epidermis, breast, prostate, ovary, cervix, hair follicle, and corneal endothelium. P-cadherin expression has also been reported on embryonic stem cells, and stem cells of the normal mammary gland, and hair follicle. Overexpression of P-cadherin has been associated with poor prognosis in breast, prostate, ovary, colon, and stomach carcinomas. Mutations in CDH3 are associated with congential hypotrichosis with juvenile macular dystrophy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

E-Cadherin Is Expressed in Epithelial Cells of the Choroid Plexus in Human and Mouse Brains.

Current issues in molecular biology

Takebayashi G,Chiba Y,Wakamatsu K,Murakami R,Miyai Y,Matsumoto K,Uemura N,Yanase K,Shirakami G,Ogino Y,Ueno M

13-2000Z was used in Immunohistochemistry to indicate that E-cadherin is expressed in the lateral membrane of CPE, possibly correlated with the expression of other cadherins and cytoplasmic proteins.

Tue Sep 26 00:00:00 EDT 2023

Mechanical stimuli-induced CCL2 restores adult mouse cells to regenerate hair follicles.

Molecular therapy. Nucleic acids

Wu W,Zhou W,Jiang J,Wang M,Zhang J,Yang J,Tang Q,Liu H,Liu D,Xu W,Zhong JL,Yang L,Lei M

13-2000Z was used in Immunohistochemistry (Paraffin) to demonstrate that CCL2 functions in immune regulation of hair regeneration under mechanical stimulus, and enhances cell multipotency during organoid culture.

Tue Jun 13 00:00:00 EDT 2023

A Cdh3-β-catenin-laminin signaling axis in a subset of breast tumor leader cells control leader cell polarization and directional collective migration.

Developmental cell

Hwang PY,Mathur J,Cao Y,Almeida J,Ye J,Morikis V,Cornish D,Clarke M,Stewart SA,Pathak A,Longmore GD

13-2000Z was used in Flow Cytometry to highlight how a subset of leader cells interact with the microenvironment to direct collective migration.

Mon Jan 09 00:00:00 EST 2023

Polysaccharide H-1-2 Ameliorates High Glucose-Induced Podocyte Dysfunction by Suppressing Epithelial-to-Mesenchymal Transition via Restoration of SIRT1 in Vivo and in Vitro.

The Tohoku journal of experimental medicine

Li M,Wu Z,Gao W,Zhao K,Yang X,Zhang H,Deng B,Niu Y

13-2000Z was used in Western Blotting to explore the effect of H-1-2, a polysaccharide of Pseudostellaria heterophylla, on high glucose (HG) induced-podocyte EMT in vivo and ex vivo.

Sat May 13 00:00:00 EDT 2023

The Mouse Model to Unravel Retinogenesis Misregulation in Patients with Albinism.

Genes

Tingaud-Sequeira A,Mercier E,Michaud V,Pinson B,Gazova I,Gontier E,Decoeur F,McKie L,Jackson IJ,Arveiler B,Javerzat S

13-2000Z was used in Immunohistochemistry to investigate the eye phenotype in dopachrome tautomerase -/- mice.

Mon Jun 27 00:00:00 EDT 2022

Apcdd1 is a dual BMP/Wnt inhibitor in the developing nervous system and skin.

Developmental biology

Vonica A,Bhat N,Phan K,Guo J,Iancu L,Weber JA,Karger A,Cain JW,Wang ECE,DeStefano GM,O'Donnell-Luria AH,Christiano AM,Riley B,Butler SJ,Luria V

13-2000Z was used in Immunocytochemistry-immunoflourescence to show that the Bone Morphogenetic Protein (BMP) and Wnt signaling pathways share Apcdd1 as a common inhibitor and that Apcdd1 is a taxon-restricted gene with novel domains and signaling functions.

Sat Aug 01 00:00:00 EDT 2020

Apcdd1 is a dual BMP/Wnt inhibitor in the developing nervous system and skin.

Developmental biology

Vonica A,Bhat N,Phan K,Guo J,Iancu L,Weber JA,Karger A,Cain JW,Wang ECE,DeStefano GM,O'Donnell-Luria AH,Christiano AM,Riley B,Butler SJ,Luria V

Sat Aug 01 00:00:00 EDT 2020

The Wave complex controls epidermal morphogenesis and proliferation by suppressing Wnt-Sox9 signaling.

The Journal of cell biology

Cohen J,Raviv S,Adir O,Padmanabhan K,Soffer A,Luxenburg C

Published figure using P-cadherin monoclonal antibody (Product # 13-2000Z) in Immunohistochemistry

Mon Apr 01 00:00:00 EDT 2019

Skin vasculature and hair follicle cross-talking associated with stem cell activation and tissue homeostasis.

eLife

Li KN,Jain P,He CH,Eun FC,Kang S,Tumbar T

13-2000Z was used in Immunohistochemistry-immunofluorescence to suggest a working model in which coordinated remodeling and molecular cross-talking of the adult epithelial and endothelial skin compartments modulate timing of HFSC activation from quiescence for proper tissue homeostasis of adult skin.

Thu Jul 25 00:00:00 EDT 2019

The epidermal polarity protein Par3 is a non-cell autonomous suppressor of malignant melanoma.

The Journal of experimental medicine

Mescher M,Jeong P,Knapp SK,Rübsam M,Saynisch M,Kranen M,Landsberg J,Schlaak M,Mauch C,Tüting T,Niessen CM,Iden S

Published figure using P-cadherin monoclonal antibody (Product # 13-2000Z) in Immunofluorescence

Wed Feb 01 00:00:00 EST 2017

The epidermal polarity protein Par3 is a non-cell autonomous suppressor of malignant melanoma.

The Journal of experimental medicine

Mescher M,Jeong P,Knapp SK,Rübsam M,Saynisch M,Kranen M,Landsberg J,Schlaak M,Mauch C,Tüting T,Niessen CM,Iden S

Published figure using P-cadherin monoclonal antibody (Product # 13-2000Z) in Immunofluorescence

Wed Feb 01 00:00:00 EST 2017

The epidermal polarity protein Par3 is a non-cell autonomous suppressor of malignant melanoma.

The Journal of experimental medicine

Mescher M,Jeong P,Knapp SK,Rübsam M,Saynisch M,Kranen M,Landsberg J,Schlaak M,Mauch C,Tüting T,Niessen CM,Iden S

Published figure using P-cadherin monoclonal antibody (Product # 13-2000Z) in Immunofluorescence

Wed Feb 01 00:00:00 EST 2017

Lhx2 is a direct NF-κB target gene that promotes primary hair follicle placode down-growth.

Development (Cambridge, England)

Tomann P,Paus R,Millar SE,Scheidereit C,Schmidt-Ullrich R

132000Z was used in immunohistochemistry - paraffin section to identify nuclear factor-kappa B targeted genes involved in hair follicle development

Sun May 01 00:00:00 EDT 2016

Retinal pigment epithelial integrity is compromised in the developing albino mouse retina.

The Journal of comparative neurology

Iwai-Takekoshi L,Ramos A,Schaler A,Weinreb S,Blazeski R,Mason C

13-2000Z was used in immunohistochemistry to utilize the developing albino mouse retina to study compromised retinal pigment epithelial integrity

Thu Dec 15 00:00:00 EST 2016

CPEB1 promotes differentiation and suppresses EMT in mammary epithelial cells.

Journal of cell science

Grudzien-Nogalska E,Reed BC,Rhoads RE

Published figure using P-cadherin monoclonal antibody (Product # 13-2000Z) in Immunofluorescence

Thu May 15 00:00:00 EDT 2014

BMI1 represses Ink4a/Arf and Hox genes to regulate stem cells in the rodent incisor.

Nature cell biology

Biehs B,Hu JK,Strauli NB,Sangiorgi E,Jung H,Heber RP,Ho S,Goodwin AF,Dasen JS,Capecchi MR,Klein OD

Published figure using P-cadherin monoclonal antibody (Product # 13-2000Z) in Immunofluorescence

Mon Jul 01 00:00:00 EDT 2013

BMI1 represses Ink4a/Arf and Hox genes to regulate stem cells in the rodent incisor.

Nature cell biology

Biehs B,Hu JK,Strauli NB,Sangiorgi E,Jung H,Heber RP,Ho S,Goodwin AF,Dasen JS,Capecchi MR,Klein OD

Published figure using P-cadherin monoclonal antibody (Product # 13-2000Z) in Immunofluorescence

Mon Jul 01 00:00:00 EDT 2013

Keratin 79 identifies a novel population of migratory epithelial cells that initiates hair canal morphogenesis and regeneration.

Development (Cambridge, England)

Veniaminova NA,Vagnozzi AN,Kopinke D,Do TT,Murtaugh LC,Maillard I,Dlugosz AA,Reiter JF,Wong SY

Sun Dec 01 00:00:00 EST 2013

Abnormal placental development and early embryonic lethality in EpCAM-null mice.

PloS one

Nagao K,Zhu J,Heneghan MB,Hanson JC,Morasso MI,Tessarollo L,Mackem S,Udey MC

13-2000Z was used in immunohistochemistry - frozen section to review data regarding EpCAM

Thu Dec 31 00:00:00 EST 2009

P-cadherin is a p63 target gene with a crucial role in the developing human limb bud and hair follicle.

Development (Cambridge, England)

Shimomura Y,Wajid M,Shapiro L,Christiano AM

13-2000Z was used in immunohistochemistry - frozen section to investigate if p63 directly interacts with two distinct regions of the P-cadherin gene CDH3 promoter

Fri Feb 01 00:00:00 EST 2008

The chromatin remodeler Mi-2beta is required for establishment of the basal epidermis and normal differentiation of its progeny.

Development (Cambridge, England)

Kashiwagi M,Morgan BA,Georgopoulos K

13-2000Z was used in immunohistochemistry to determine the role of Mi-2beta in skin development

Sun Apr 01 00:00:00 EDT 2007

Cadherin expression in the developing mouse olfactory system.

The Journal of comparative neurology

Akins MR,Benson DL,Greer CA

13-2000Z was used in immunohistochemistry to report the localization of classical cadherins in the developing olfactory system.

Sun Apr 01 00:00:00 EDT 2007

Tissue surface tensions guide in vitro self-assembly of rodent pancreatic islet cells.

Developmental dynamics : an official publication of the American Association of Anatomists

Jia D,Dajusta D,Foty RA

13-2000Z was used in flow cytometry to investigate how differential adhesion alters the organization of immortalized rodent pancreatic islet cells

Wed Aug 01 00:00:00 EDT 2007

WT1-interacting protein and ZO-1 translocate into podocyte nuclei after puromycin aminonucleoside treatment.

American journal of physiology. Renal physiology

Rico M,Mukherjee A,Konieczkowski M,Bruggeman LA,Miller RT,Khan S,Schelling JR,Sedor JR

Mon Aug 01 00:00:00 EDT 2005

WT1-interacting protein and ZO-1 translocate into podocyte nuclei after puromycin aminonucleoside treatment.

American journal of physiology. Renal physiology

Rico M,Mukherjee A,Konieczkowski M,Bruggeman LA,Miller RT,Khan S,Schelling JR,Sedor JR

Mon Aug 01 00:00:00 EDT 2005

WT1-interacting protein and ZO-1 translocate into podocyte nuclei after puromycin aminonucleoside treatment.

American journal of physiology. Renal physiology

Rico M,Mukherjee A,Konieczkowski M,Bruggeman LA,Miller RT,Khan S,Schelling JR,Sedor JR

Mon Aug 01 00:00:00 EDT 2005

Conditional targeting of E-cadherin in skin: insights into hyperproliferative and degenerative responses.

Proceedings of the National Academy of Sciences of the United States of America

Tinkle CL,Lechler T,Pasolli HA,Fuchs E

Tue Jan 13 00:00:00 EST 2004

Conditional targeting of E-cadherin in skin: insights into hyperproliferative and degenerative responses.

Proceedings of the National Academy of Sciences of the United States of America

Tinkle CL,Lechler T,Pasolli HA,Fuchs E

Tue Jan 13 00:00:00 EST 2004

Links between signal transduction, transcription and adhesion in epithelial bud development.

Nature

Jamora C,DasGupta R,Kocieniewski P,Fuchs E

Thu Mar 20 00:00:00 EST 2003

Neph1 and nephrin interaction in the slit diaphragm is an important determinant of glomerular permeability.

The Journal of clinical investigation

Liu G,Kaw B,Kurfis J,Rahmanuddin S,Kanwar YS,Chugh SS

Tue Jul 01 00:00:00 EDT 2003

Neph1 and nephrin interaction in the slit diaphragm is an important determinant of glomerular permeability.

The Journal of clinical investigation

Liu G,Kaw B,Kurfis J,Rahmanuddin S,Kanwar YS,Chugh SS

Tue Jul 01 00:00:00 EDT 2003

Cadherin-mediated cell adhesion and tissue segregation: qualitative and quantitative determinants.

Developmental biology

Duguay D,Foty RA,Steinberg MS

13-2000Z was used in flow cytometry to examine cadherin-subtype-specific binding specificities.

Wed Jan 15 00:00:00 EST 2003

Genetic dissection of cadherin function during nephrogenesis.

Molecular and cellular biology

Dahl U,Sjödin A,Larue L,Radice GL,Cajander S,Takeichi M,Kemler R,Semb H

13-2000Z was used in immunohistochemistry to determine if R-cadherin is required for kidney ontogeny.

Fri Mar 01 00:00:00 EST 2002

Ectopic expression of PA2.26 antigen in epidermal keratinocytes leads to destabilization of adherens junctions and malignant progression.

Laboratory investigation; a journal of technical methods and pathology

Scholl FG,Gamallo C,Quintanilla M

13-2000Z was used in western blot to report that expression of the PA2.26 antigen by premalignant keratinocytes induces a fully transformed and metastatic phenotype.

Wed Nov 01 00:00:00 EST 2000

Actin 'purse string' filaments are anchored by E-cadherin-mediated adherens junctions at the leading edge of the epithelial wound, providing coordinated cell movement.

Journal of cell science

Danjo Y,Gipson IK

13-2000Z was used in immunohistochemistry to elucidate the role of the actin filament cable in wound healing.

Sun Nov 01 00:00:00 EST 1998

The cadherins: cell-cell adhesion molecules controlling animal morphogenesis.

Development (Cambridge, England)

Takeichi M

Fri Apr 01 00:00:00 EST 1988

The cadherins: cell-cell adhesion molecules controlling animal morphogenesis.

Development (Cambridge, England)

Takeichi M

Fri Apr 01 00:00:00 EST 1988

The cadherins: cell-cell adhesion molecules controlling animal morphogenesis.

Development (Cambridge, England)

Takeichi M

Fri Apr 01 00:00:00 EST 1988

Bioinformatics

Protein Aliases: CADH3; Cadherin-3; P-cadherin; Placental cadherin; Placental-cadherin; RPE-specific cadherin

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Gene Aliases: AI385538; Cadp; Cdh3; Cdhp; P-cadherin; Pcad

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UniProt ID: (Mouse) P10287

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Entrez Gene ID: (Mouse) 12560

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Function(s)

calcium ion binding metal ion binding cadherin

Process(es)

retina homeostasis cell adhesion homophilic cell adhesion via plasma membrane adhesion molecules positive regulation of gene expression positive regulation of keratinocyte proliferation single organismal cell-cell adhesion hair cycle process keratinization positive regulation of monophenol monooxygenase activity negative regulation of transforming growth factor beta2 production positive regulation of insulin-like growth factor receptor signaling pathway positive regulation of melanin biosynthetic process negative regulation of catagen canonical Wnt signaling pathway regulation of hair cycle by canonical Wnt signaling pathway positive regulation of melanosome transport

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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P-cadherin Monoclonal Antibody (PCD-1)

P-cadherin Antibody (13-2000Z) in IHC

Product Details

13-2000Z

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Inhibition Assays (IA)

In vitro Assay (IV)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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