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P2X1 Antibody (PA5-114312) in ICC/IF
Immunocytochemical analysis of P2X1 in SKOV-3 cells using a P2X1 polyclonal antibody (Product #PA5-114312). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1:1,000 dilution. The nuclear counter stain is DAPI.
Product Details
PA5-114312
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide within Human P2X1 aa 252-301
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 0.2% BSA, 50% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2890481
Target Information
The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel with relatively high calcium permeability. Binding to ATP mediates synaptic transmission between neurons and from neurons to smooth muscle, being responsible, for example, for sympathetic vasoconstriction in small arteries, arterioles and vas deferens. Mouse studies suggest that this receptor is essential for normal male reproductive function. It is possible that the development of selective antagonists for this receptor may provide an effective non-hormonal male contraceptive pill.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATP receptor; P2X purinoceptor 1; P2X receptor, subunit 1; P2X1; P2X1 receptor; programmed cell death 3; Purinergic receptor; purinergic receptor P2X, ligand gated ion channel, 1; purinergic receptor P2X, ligand-gated ion channel, 1; purinergic receptor P2X1; RP-2 protein
Gene Aliases: AI323649; BB122383; P2RX1; P2x; P2X1; P2XMR; Pdcd3; RP-2
UniProt ID: (Human) P51575, (Rat) P47824, (Mouse) P51576
Entrez Gene ID: (Human) 5023, (Rat) 25505, (Mouse) 18436
serotonin secretion by platelet
regulation of vascular smooth muscle contraction
transport
ion transport
apoptotic process
activation of cysteine-type endopeptidase activity involved in apoptotic process
signal transduction
insemination
blood coagulation
regulation of blood pressure
neuronal action potential
platelet activation
response to ATP
synaptic transmission, glutamatergic
purinergic nucleotide receptor signaling pathway
vasoconstriction
positive regulation of ion transport
ceramide biosynthetic process
protein homooligomerization
protein heterooligomerization
regulation of calcium ion transport
cation transmembrane transport
cation transport
regulation of smooth muscle contraction
response to organic substance
regulation of vasoconstriction
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