Proteintech

P53 Monoclonal Antibody (6C4B6)

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View all (369) p53 antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

60283-2-IG

Applications
Tested Dilution
Publications

Western Blot (WB)

1:2,000-1:10,000
View 118 publications 118 publications

Immunohistochemistry (IHC)

-
View 18 publications 18 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:1,600-1:6,400
-

Immunocytochemistry (ICC/IF)

-
View 15 publications 15 publications

Immunoprecipitation (IP)

-
View 7 publications 7 publications

Not applicable (N/A)

-
View 2 publications 2 publications
Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

6C4B6

Immunogen

P53 Fusion Protein Ag0698 (1-232 aa encoded by BC003596)
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MEEPQSDPS VEPPLSQETF SDLWKLLPEN NVLSPLPSQA MDDLMLSPDD IEQWFTEDPG PDEAPRMPEA APRVAPAPAA PTPAAPAPAP SWPLSSSVPS QKTYQGSYGF RLGFLHSGTA KSVTCTYSPA LNKMFCQLAK TCPVQLWVDS TPPPGTRVRA MAIYKQSQHM TEVVRRCPHH ERCSDSDGLA PPQHLIRVEG NLRVEYLDDR NTFRHSVVVP YEPPEVGSDC TTI (1-232 aa encoded by BC003596)

Target Information

The tumor suppressor protein, p53, is a sequence specific transcription factor that is activated by cellular stress. p53 mediates cell cycle arrest or apoptosis in response to DNA damage or starvation for pyrimidine nucleotides. p53 is up-regulated in response to stress signals and stimulated to activate transcription of specific genes, resulting in expression of p21waf1 and other proteins involved in G1 or G2/M arrest. The structure of p53 comprises an N-terminal transactivation domain, a central DNA-binding domain, an oligomerisation domain, and a C-terminal regulatory domain. There are various phosphorylation sites on p53, of which the phosphorylation at Ser15 is important for p53 activation and stabilization. p53 has been characterized to play a role in blocking the proliferative action of damaged cells and act as an anticancer agent. Phosphorylation of Ser392 in p53 has been shown to associate with the formation of human tumors. In addition, p53 has also been linked to the effects of aging and oxidative stress and an increase in p53 has been linked to deficits in LTP (Long Term Potentiation) in learning and memory. p53 is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and cause the loss of tumor suppressor activity. Alterations of the TP53 gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families such as Li-Fraumeni syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Antigen NY-CO-13; Cellular tumor antigen p53; Cys 51 Stop; FLJ92943; HGNC11998; mutant p53; mutant tumor protein 53; OTTMUSP00000006194; p53 cellular tumor antigen; p53 tumor suppressor; Phosphoprotein p53; transformation related protein 53; transformation-related protein 53; tumor protein 53; tumor protein p53 (Li-Fraumeni syndrome); Tumor suppressor p53; tumor supressor p53; Tumour Protein p53

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Gene Aliases: bbl; BCC7; bfy; bhy; LFS1; p44; P53; TP53; TRP53

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UniProt ID: (Human) P04637, (Rat) P10361, (Mouse) P02340

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Entrez Gene ID: (Human) 7157, (Rat) 24842, (Mouse) 22059

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Function(s)
RNA polymerase II transcription factor activity, sequence-specific DNA binding core promoter sequence-specific DNA binding RNA polymerase II transcription factor binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding protease binding p53 binding DNA binding chromatin binding damaged DNA binding double-stranded DNA binding transcription factor activity, sequence-specific DNA binding copper ion binding protein binding ATP binding transcription factor binding zinc ion binding enzyme binding protein kinase binding protein phosphatase binding receptor tyrosine kinase binding ubiquitin protein ligase binding histone acetyltransferase binding identical protein binding sequence-specific DNA binding protein self-association transcription regulatory region DNA binding protein heterodimerization activity protein N-terminus binding chaperone binding protein phosphatase 2A binding RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcription cofactor binding protein C-terminus binding histone deacetylase regulator activity MDM2/MDM4 family protein binding metal ion binding P53-like transcription factor
Process(es)
negative regulation of transcription from RNA polymerase II promoter DNA strand renaturation base-excision repair nucleotide-excision repair regulation of transcription, DNA-templated transcription from RNA polymerase II promoter protein complex assembly cellular response to DNA damage stimulus DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator ER overload response cell cycle arrest Ras protein signal transduction multicellular organism development cell aging protein localization cell proliferation negative regulation of cell proliferation determination of adult lifespan response to X-ray response to gamma radiation positive regulation of gene expression viral process protein sumoylation cell differentiation negative regulation of cell growth DNA damage response, signal transduction by p53 class mediator positive regulation of histone deacetylation chromatin assembly mitotic G1 DNA damage checkpoint positive regulation of protein oligomerization cellular response to UV cellular response to drug cellular response to glucose starvation intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator regulation of apoptotic process positive regulation of apoptotic process negative regulation of apoptotic process entrainment of circadian clock by photoperiod proteasome-mediated ubiquitin-dependent protein catabolic process positive regulation of neuron apoptotic process negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter response to antibiotic positive regulation of protein export from nucleus regulation of mitochondrial membrane permeability negative regulation of fibroblast proliferation circadian behavior positive regulation of peptidyl-tyrosine phosphorylation negative regulation of helicase activity protein tetramerization negative regulation of telomerase activity positive regulation of thymocyte apoptotic process positive regulation of cell cycle arrest cellular response to hypoxia cellular response to ionizing radiation intrinsic apoptotic signaling pathway by p53 class mediator positive regulation of release of cytochrome c from mitochondria replicative senescence oxidative stress-induced premature senescence intrinsic apoptotic signaling pathway oligodendrocyte apoptotic process positive regulation of execution phase of apoptosis positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway regulation of signal transduction by p53 class mediator regulation of cell cycle G2/M phase transition positive regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress positive regulation of reactive oxygen species metabolic process positive regulation of intrinsic apoptotic signaling pathway protein import into nucleus, translocation in utero embryonic development somitogenesis release of cytochrome c from mitochondria T cell proliferation involved in immune response B cell lineage commitment T cell lineage commitment positive regulation of leukocyte migration response to ischemia double-strand break repair regulation of transcription from RNA polymerase II promoter apoptotic process response to oxidative stress transforming growth factor beta receptor signaling pathway gastrulation negative regulation of neuroblast proliferation central nervous system development aging negative regulation of DNA replication rRNA transcription response to UV response to salt stress embryo development ending in birth or egg hatching response to inorganic substance response to metal ion response to UV-B response to organonitrogen compound positive regulation of cardiac muscle cell apoptotic process response to organic cyclic compound cerebellum development negative regulation of transforming growth factor beta receptor signaling pathway response to caffeine response to retinoic acid T cell differentiation in thymus response to vitamin B3 response to cytokine regulation of tissue remodeling cellular response to heat cellular protein localization multicellular organism growth positive regulation of mitochondrial membrane permeability wound healing regulation of cell proliferation response to drug response to amino acid mitochondrial DNA repair regulation of neuron apoptotic process positive regulation of cell cycle negative regulation of proteolysis negative regulation of mitotic cell cycle embryonic organ development negative regulation of smooth muscle cell proliferation regulation of catalytic activity chromosome organization neuron apoptotic process regulation of intracellular pH regulation of cell cycle response to hyperoxia regulation of thymocyte apoptotic process necroptotic process cellular response to organonitrogen compound mitotic cell cycle arrest positive regulation of cell aging negative regulation of macromitophagy regulation of mitochondrial membrane permeability involved in apoptotic process regulation of intrinsic apoptotic signaling pathway by p53 class mediator regulation of hydrogen peroxide-induced cell death regulation of fibroblast apoptotic process negative regulation of DNA biosynthetic process negative regulation of reactive oxygen species metabolic process regulation of cellular senescence transcription, DNA-templated cell cycle programmed cell death chromosome breakage rhythmic process positive regulation of transcription from RNA polymerase II promoter in response to hypoxia intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress cellular response to UV-C regulation of glycolytic process by positive regulation of transcription from RNA polymerase II promoter negative regulation of glucose catabolic process to lactate via pyruvate intrinsic apoptotic signaling pathway in response to hypoxia
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