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Invitrogen
VPS35 Polyclonal Antibody
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 13
VPS35 Antibody (PA5-21898) in ICC/IF
Immunofluorescence analysis of VPS35 was performed using U-87 MG cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with VPS35 Rabbit Polyclonal Antibody (Product # PA5-21898) at 1:200 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of... View More
Product Details
PA5-21898
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to a region within amino acids 733 and 796 of VPS35 (Uniprot ID#Q96QK1)
Conjugate
Form
Liquid
Concentration
0.66 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_11153540
Product Specific Information
Recommended positive controls: Mouse brain, Rat Brain, Hela, A549, H1299, HCT116, HepG2, Molt4, Raji.
Predicted reactivity: Mouse (100%), Rat (100%), Bovine (100%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: DKFZp434E1211; DKFZp434P1672; FLJ10752; FLJ13588; FLJ20388; hVPS35; Maternal embryonic message 3; Maternal-embryonic 3; vacuolar protein sorting 35 homolog; Vacuolar protein sorting-associated protein 35; Vesicle protein sorting 35
Gene Aliases: AI647796; MEM3; PARK17; TCCCTA00141; VPS35
UniProt ID: (Human) Q96QK1, (Mouse) Q9EQH3
Entrez Gene ID: (Human) 55737, (Rat) 25479, (Mouse) 65114
intracellular protein transport
Wnt signaling pathway
regulation of macroautophagy
regulation of protein stability
retrograde transport, endosome to Golgi
transcytosis
positive regulation of Wnt protein secretion
positive regulation of canonical Wnt signaling pathway
negative regulation of late endosome to lysosome transport
retrograde transport, endosome to plasma membrane
vacuolar protein processing
transport
protein transport
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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