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Invitrogen
TREM2 Polyclonal Antibody
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 7
TREM2 Antibody (PA5-46978) in ICC/IF
Immunofluorescence analysis of TREM2 was performed using 70% confluent log phase RAW 264.7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with TREM2 Polyclonal Antibody (Product # PA5-46978) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Sheep IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 (Product # A11015), (1:... View More
Product Details
PA5-46978
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Sheep
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse TREM-2b Leu19-Pro168
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2605803
Product Specific Information
In direct ELISAs, approximately 30% cross-reactivity with recombinant human TREM-2 is observed and less than 1% cross-reactivity with recombinant mouse (rm) TREM-1 and rmTREM-3 is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Target Information
TREM2 is a membrane protein that forms a receptor signaling complex with TYROBP. The encoded protein may be involved in chronic inflammation by triggering the production of constitutive inflammatory cytokines. Defects in this gene are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: OTTHUMP00000039771; soluble TREM 2; soluble TREM2; sTREM 2; sTREM2; TREM; TREM-2; Triggering receptor expressed on monocytes 2; Triggering receptor expressed on myeloid cells; Triggering receptor expressed on myeloid cells 2; triggering receptor expressed on myeloid cells 2a; triggering receptor expressed on myeloid cells 2b; triggering receptor expressed on myeloid cells 2c
Gene Aliases: TREM-2; Trem2; Trem2a; Trem2b; Trem2c
UniProt ID: (Mouse) Q99NH8
Entrez Gene ID: (Mouse) 83433
positive regulation of antigen processing and presentation of peptide antigen via MHC class II
signal transduction
lipopolysaccharide-mediated signaling pathway
detection of lipopolysaccharide
detection of peptidoglycan
innate immune response
positive regulation of peptidyl-tyrosine phosphorylation
positive regulation of calcium-mediated signaling
positive regulation of ERK1 and ERK2 cascade
detection of lipoteichoic acid
cellular response to lipoteichoic acid
cellular response to peptidoglycan
dendritic cell differentiation
positive regulation of protein localization to plasma membrane
positive regulation of C-C chemokine receptor CCR7 signaling pathway
positive regulation of CD40 signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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