Invitrogen

DJ-1 Polyclonal Antibody

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This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

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Datasheet
Protocols
Questions & Answers
Tech Support

Product Details

PA5-78363

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:20,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human Park7/DJ-1. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.91 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol, 1% BSA

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2735724

Product Specific Information

Positive Control: 293T, A431, HeLa, HepG2, mouse pancreas

Predicted Reactivity: Zebrafish (83%), Pig (96%), Chicken (89%), Bovine (96%)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

The product of this gene belongs to the peptidase C56 family of proteins. It acts as a positive regulator of androgen receptor-dependent transcription. It may also function as a redox-sensitive chaperone, as a sensor for oxidative stress, and it apparently protects neurons against oxidative stress and cell death. Defects in this gene are the cause of autosomal recessive early-onset Parkinson disease 7. Two transcript variants encoding the same protein have been identified for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Contraception-associated protein 1; CTA-215D11.1; DJ-1; early onset 7; epididymis secretory sperm binding protein Li 67p; Fertility protein SP22; FLJ27376; FLJ34360; FLJ92274; Maillard deglycase; Oncogene DJ1; OTTHUMP00000001350; OTTHUMP00000001351; PARK7 GENE; PARK7/DJ1; Parkinson disease (autosomal recessive, early onset) 7; Parkinson disease protein 7; Parkinson disease protein 7 homolog; parkinson protein 7; Parkinsonism-associated deglycase; Protein CAP1; protein deglycase DJ-1; Protein DJ-1; Protein/nucleic acid deglycase DJ-1; RNA-binding protein regulatory subunit

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Gene Aliases: CAP1; DJ-1; DJ1; HEL-S-67p; PARK7; SP22

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UniProt ID: (Human) Q99497, (Rat) O88767, (Mouse) Q99LX0

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Entrez Gene ID: (Human) 11315, (Rat) 117287, (Mouse) 57320

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Function(s)
core promoter binding double-stranded DNA binding single-stranded DNA binding transcription coactivator activity mRNA binding receptor binding copper ion binding protein binding transcription factor binding peptidase activity superoxide dismutase copper chaperone activity oxidoreductase activity, acting on peroxide as acceptor glyoxalase III activity enzyme binding kinase binding cytokine binding tyrosine 3-monooxygenase activator activity L-dopa decarboxylase activator activity identical protein binding protein homodimerization activity small protein activating enzyme binding ubiquitin-like protein conjugating enzyme binding mercury ion binding androgen receptor binding peroxiredoxin activity repressing transcription factor binding scaffold protein binding cadherin binding involved in cell-cell adhesion cupric ion binding cuprous ion binding ubiquitin-specific protease binding glyoxalase (glycolic acid-forming) activity molecular_function RNA binding histone deacetylase activity protein-N-terminal asparagine amidohydrolase activity UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase activity CoA hydrolase activity hydrolase activity iprodione amidohydrolase activity (3,5-dichlorophenylurea)acetate amidohydrolase activity 4'-(2-hydroxyisopropyl)phenylurea amidohydrolase activity didemethylisoproturon amidohydrolase activity N-isopropylacetanilide amidohydrolase activity N-cyclohexylformamide amidohydrolase activity 2-oxoglutaryl-CoA thioesterase activity 2,4,4-trimethyl-3-oxopentanoyl-CoA thioesterase activity isonicotinic acid hydrazide hydrolase activity cis-aconitamide amidase activity gamma-N-formylaminovinylacetate hydrolase activity 3-isopropylbut-3-enoyl-CoA thioesterase activity N2-acetyl-L-lysine deacetylase activity O-succinylbenzoate synthase activity indoleacetamide hydrolase activity N-acetylcitrulline deacetylase activity glutaryl-CoA hydrolase activity N-acetylgalactosamine-6-phosphate deacetylase activity acyl-CoA hydrolase activity diacetylchitobiose deacetylase activity chitooligosaccharide deacetylase activity
Process(es)
negative regulation of protein phosphorylation synaptic transmission, dopaminergic negative regulation of protein kinase activity proteolysis protein deglycosylation autophagy inflammatory response response to oxidative stress mitochondrion organization single fertilization adult locomotory behavior fertilization detoxification of copper ion negative regulation of gene expression methylglyoxal catabolic process to D-lactate via S-lactoyl-glutathione lactate biosynthetic process negative regulation of protein ubiquitination negative regulation of protein binding negative regulation of proteasomal ubiquitin-dependent protein catabolic process positive regulation of interleukin-8 production positive regulation of peptidyl-serine phosphorylation negative regulation of protein sumoylation cellular response to oxidative stress cellular response to reactive oxygen species cellular response to glyoxal response to drug response to hydrogen peroxide hydrogen peroxide metabolic process negative regulation of apoptotic process regulation of neuron apoptotic process negative regulation of neuron apoptotic process regulation of TRAIL receptor biosynthetic process positive regulation of transcription from RNA polymerase II promoter glycolate biosynthetic process negative regulation of protein export from nucleus regulation of inflammatory response detoxification of mercury ion protein stabilization positive regulation of sequence-specific DNA binding transcription factor activity negative regulation of ubiquitin-protein transferase activity dopamine uptake involved in synaptic transmission regulation of mitochondrial membrane potential membrane depolarization oxidation-reduction process membrane hyperpolarization negative regulation of cell death regulation of androgen receptor signaling pathway cellular response to hydrogen peroxide positive regulation of protein homodimerization activity positive regulation of protein localization to nucleus negative regulation of neuron death positive regulation of superoxide dismutase activity negative regulation of protein acetylation positive regulation of oxidative stress-induced intrinsic apoptotic signaling pathway negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway positive regulation of mitochondrial electron transport, NADH to ubiquinone negative regulation of protein K48-linked deubiquitination negative regulation of TRAIL-activated apoptotic signaling pathway positive regulation of pyrroline-5-carboxylate reductase activity positive regulation of tyrosine 3-monooxygenase activity positive regulation of dopamine biosynthetic process glyoxal catabolic process positive regulation of L-dopa biosynthetic process positive regulation of L-dopa decarboxylase activity negative regulation of oxidative stress-induced cell death negative regulation of hydrogen peroxide-induced cell death negative regulation of hydrogen peroxide-induced neuron death negative regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway negative regulation of hydrogen peroxide-induced neuron intrinsic apoptotic signaling pathway positive regulation of reactive oxygen species biosynthetic process negative regulation of ubiquitin-specific protease activity positive regulation of oxidative phosphorylation uncoupler activity positive regulation of transcription regulatory region DNA binding positive regulation of androgen receptor activity negative regulation of extrinsic apoptotic signaling pathway negative regulation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway cell proliferation positive regulation of gene expression enzyme active site formation via L-cysteine sulfinic acid negative regulation of protein catabolic process negative regulation of oxidative stress-induced neuron death Ras protein signal transduction protein repair activation of protein kinase B activity positive regulation of protein kinase B signaling cell-cell adhesion cellular oxidant detoxification regulation of fibril organization negative regulation of death-inducing signaling complex assembly positive regulation of mitophagy negative regulation of nitrosative stress-induced intrinsic apoptotic signaling pathway
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