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Invitrogen
Huntingtin Polyclonal Antibody
Advanced Verification
This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 14
Huntingtin Antibody (PA5-85721) in ICC/IF
Immunofluorescence analysis of Huntingtin was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Huntingtin Polyclonal Antibody (Product # PA5-85721) at 1:100 dilution in 0.1% BSA, incubated at 4 degree... View More
Product Details
PA5-85721
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Huntingtin.
Conjugate
Form
Liquid
Concentration
1.4 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2792860
Product Specific Information
Keep as concentrated solution.
Predicted reactivity: Mouse (100%), Rat (100%), Pig (100%), Sheep (100%).
Positive Control: U87-MG.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Huntingtin is a disease gene linked to Huntington's disease (HD), a neurodegenerative disorder characterized by loss of striatal neurons. This is thought to be caused by an expanded, unstable trinucleotide repeat in the huntingtin gene, which translates as a polyglutamine repeat in the protein product. HD is a mid-life onset autosomal dominant neurodegenerative disease that is characterized by psychiatric disorders, dementia, and involuntary movements (chorea), leading to death in 10-20 years.The huntingtin locus is large, spanning 180 kb and consisting of 67 exons. The huntingtin gene is widely expressed and is required for normal development. It is expressed as 2 alternatively polyadenylated forms displaying different relative abundance in various fetal and adult tissues. The larger transcript is approximately 13.7 kb and is expressed predominantly in adult and fetal brain whereas the smaller transcript of approximately 10.3 kb is more widely expressed. The genetic defect leading to Huntington's disease may not necessarily eliminate transcription, but may confer a new property on the mRNA or alter the function of the protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: HD protein; HD protein homolog; Huntingtin; huntingtin (Huntington disease); Huntington disease gene homolog; Huntington disease protein; huntington disease protein homolog
Gene Aliases: HD; Hdh; HTT; IT15
UniProt ID: (Human) P42858
Entrez Gene ID: (Human) 3064, (Rat) 29424
urea cycle
citrulline metabolic process
establishment of mitotic spindle orientation
protein import into nucleus
mitochondrial transport
ER to Golgi vesicle-mediated transport
retrograde vesicle-mediated transport, Golgi to ER
apoptotic process
mitochondrion organization
endoplasmic reticulum organization
Golgi organization
dopamine receptor signaling pathway
spermatogenesis
gastrulation
central nervous system development
brain development
cell aging
learning or memory
learning
grooming behavior
locomotory behavior
axo-dendritic transport
associative learning
determination of adult lifespan
visual learning
anatomical structure morphogenesis
anterior/posterior pattern specification
endosomal transport
lactate biosynthetic process from pyruvate
quinolinate biosynthetic process
striatum development
olfactory lobe development
neural plate formation
neurogenesis
peptide hormone secretion
insulin secretion
positive regulation of inositol 1,4,5-trisphosphate-sensitive calcium-release channel activity
regulation of protein phosphatase type 2A activity
social behavior
hormone metabolic process
negative regulation of apoptotic process
negative regulation of neuron apoptotic process
regulation of mitochondrial membrane permeability
vesicle transport along microtubule
regulation of synaptic plasticity
paraxial mesoderm formation
animal organ development
neuron development
mRNA transport
neuron apoptotic process
response to calcium ion
regulation of mitochondrial membrane potential
L-glutamate import
iron ion homeostasis
negative regulation of neuron death
negative regulation of cysteine-type endopeptidase activity
negative regulation of extrinsic apoptotic signaling pathway
vocal learning
positive regulation of cilium assembly
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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