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PAF49 Antibody (A301-295A) in ICC/IF
Detection of human PAF49 by immunocytochemistry. Sample: Formaldehyde-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti- PAF49 (Cat. No.A301-295A Lot1) used at a dilution of 1:100 (2 µg/mL). Detection: Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody used at a dilution of 1:100.
Product Details
A301-295A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 404 and 454 of human RNA polymerase I-associated factor PAF49 (CD3-epsilon-associated protein).
Conjugate
Form
Liquid
Concentration
0.20 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
TBS, pH 7.0 to 8.0, with 0.1% BSA
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Wet ice
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Application Note: For ICC, formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells. For IHC, epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.
Target Information
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Isoform 1 is involved in UBTF-activated transcription, presumably at a step following PIC formation.; Isoform 2 has been described as a component of preformed T-cell receptor (TCR) complex. [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: A34.5; antis; Antisense to ERCC-1 protein; ASE-1; CD3-epsilon-associated protein; CD3e antigen, epsilon polypeptide associated protein; CD3e molecule, epsilon associated protein; CD3E-associated protein; DNA-directed RNA polymerase I subunit G; DNA-directed RNA polymerase I subunit RPA34; RNA polymerase I-associated factor PAF49
Gene Aliases: ASE-1; ASE1; CAST; CD3EAP; PAF49; POLR1G
UniProt ID: (Human) O15446
Entrez Gene ID: (Human) 10849
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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