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PAFAH Polyclonal Antibody

PAFAH Antibody in Immunocytochemistry (ICC/IF)

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PAFAH Antibody (15526-1-AP) in ICC/IF

Immunofluorescent analysis of ( -20°C Ethanol ) fixed RAW 264.7 cells using 15526-1-AP (PAFAH antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

PAFAH Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

15526-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200-1:1,000

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:50-1:500

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

PAFAH Fusion Protein Ag7227 (92-438 aa encoded by BC038452)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: RLDTLWIPN KEYFWGLSKF LGTHWLMGNI LRLLFGSMTT PANWNSPLRP GEKYPLVVFS HGLGAFRTLY SAIGIDLASH GFIVAAVEHR DRSASATYYF KDQSAAEIGD KSWLYLRTLK QEEETHIRNE QVRQRAKECS QALSLILDID HGKPVKNALD LKFDMEQLKD SIDREKIAVI GHSFGGATVI QTLSEDQRFR CGIALDAWMF PLGDEVYSRI PQPLFFINSE YFQYPANIIK MKKCYSPDKE RKMITIRGSV HQNFADFTFA TGKIIGHMLK LKGDIDSNAA IDLSNKASLA FLQKHLGLHK DFDQWDCLIE GDDENLIPGT NINTTNQHIM LQNSSGIE (92-438 aa encoded by BC038452 )

Target Information

Platelet Activating Factor (PAF) is a biologically active phospholipid, which exerts primarily proinflammatory activities by specifically signaling through G-protein-coupled receptors on platelets, neutrophils, and monocytes. Platelet Activating Factor Acetylhydrolase (PAF-AH) is a secreted protein that mediates PAF activity by specifically catalyzing hydrolysis of the "sn2" ester bond, resulting in the conversion of PAF to the biologically inactive lyso-PAF. PAF-AH can also interact with LDL particles to induce the hydrolysis of LDL-associated, oxidized phospholipids, generating lysophosphatidylcholine (lyso-PC) and other lysophospholipids.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

INPP5D/SHIP1 regulates inflammasome activation in human microglia.

bioRxiv : the preprint server for biology

Chou V,Fancher SB,Pearse RV,Lee H,Lam M,Seyfried NT,Bennett DA,De Jager PL,Menon V,Young-Pearse TL

Sun Feb 26 00:00:00 EST 2023

PLA2G7/PAF-AH as Potential Negative Regulator of the Wnt Signaling Pathway Mediates Protective Effects in BRCA1 Mutant Breast Cancer.

International journal of molecular sciences

Liao Y,Badmann S,Kraus F,Topalov NE,Mayr D,Kolben T,Hester A,Beyer S,Mahner S,Jeschke U,Trillsch F,Czogalla B,Burges A

Tue Jan 03 00:00:00 EST 2023

Characterizing cellular heterogeneity in fibrotic hypersensitivity pneumonitis by single-cell transcriptional analysis.

Cell death discovery

Wang J,Zhang L,Luo L,He P,Xiong A,Jiang M,Liu Y,Liu S,Ran Q,Wu D,Xiong Y,He X,Li G

Fri Jan 28 00:00:00 EST 2022

Elevated dietary ω-6 polyunsaturated fatty acids induce reversible peripheral nerve dysfunction that exacerbates comorbid pain conditions.

Nature metabolism

Boyd JT,LoCoco PM,Furr AR,Bendele MR,Tram M,Li Q,Chang FM,Colley ME,Samenuk GM,Arris DA,Locke EE,Bach SBH,Tobon A,Ruparel SB,Hargreaves KM

Tue Jun 01 00:00:00 EDT 2021

Elevated dietary ω-6 polyunsaturated fatty acids induce reversible peripheral nerve dysfunction that exacerbates comorbid pain conditions.

Nature metabolism

Boyd JT,LoCoco PM,Furr AR,Bendele MR,Tram M,Li Q,Chang FM,Colley ME,Samenuk GM,Arris DA,Locke EE,Bach SBH,Tobon A,Ruparel SB,Hargreaves KM

Tue Jun 01 00:00:00 EDT 2021

Characterization of protein, long noncoding RNA and microRNA signatures in extracellular vesicles derived from resting and degranulated mast cells.

Journal of extracellular vesicles

Liang Y,Huang S,Qiao L,Peng X,Li C,Lin K,Xie G,Li J,Lin L,Yin Y,Liao H,Li Q,Li L

Sat Oct 24 00:00:00 EDT 2020

VDAC2 enables BAX to mediate apoptosis and limit tumor development.

Nature communications

Chin HS,Li MX,Tan IKL,Ninnis RL,Reljic B,Scicluna K,Dagley LF,Sandow JJ,Kelly GL,Samson AL,Chappaz S,Khaw SL,Chang C,Morokoff A,Brinkmann K,Webb A,Hockings C,Hall CM,Kueh AJ,Ryan MT,Kluck RM,Bouillet P,Herold MJ,Gray DHD,Huang DCS,van Delft MF,Dewson G

Mon Nov 26 00:00:00 EST 2018

VDAC2 enables BAX to mediate apoptosis and limit tumor development.

Nature communications

Mon Nov 26 00:00:00 EST 2018

Stromal-epithelial crosstalk regulates kidney progenitor cell differentiation.

Nature cell biology

Das A,Tanigawa S,Karner CM,Xin M,Lum L,Chen C,Olson EN,Perantoni AO,Carroll TJ

Sun Sep 01 00:00:00 EDT 2013

Stromal-epithelial crosstalk regulates kidney progenitor cell differentiation.

Nature cell biology

Das A,Tanigawa S,Karner CM,Xin M,Lum L,Chen C,Olson EN,Perantoni AO,Carroll TJ

Sun Sep 01 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: 1-alkyl-2-acetylglycerophosphocholine esterase; 2-acetyl-1-alkylglycerophosphocholine esterase; Group-VIIA phospholipase A2; gVIIA-PLA2; LDL-associated phospholipase A2; LDL-PLA(2); lipoprotein-associated phospholipase A2; PAF 2-acylhydrolase; PAF acetylhydrolase; phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma); Platelet-activating factor acetylhydrolase

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Gene Aliases: LDL-PLA2; LP-PLA2; PAFAD; PAFAH; PLA2G7; R75400

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UniProt ID: (Human) Q13093, (Mouse) Q60963

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Entrez Gene ID: (Human) 7941, (Mouse) 27226

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Function(s)

1-alkyl-2-acetylglycerophosphocholine esterase activity phospholipid binding hydrolase activity, acting on ester bonds calcium-independent phospholipase A2 activity hydrolase activity protein modifying enzyme

Process(es)

lipid catabolic process low-density lipoprotein particle remodeling lipid oxidation plasma lipoprotein particle oxidation positive regulation of inflammatory response positive regulation of monocyte chemotaxis lipid metabolic process inflammatory response

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PAFAH Polyclonal Antibody

PAFAH Antibody (15526-1-AP) in ICC/IF

Product Details

15526-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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