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PAK1 Antibody (PA5-118139) in ICC/IF
Immunocytochemistry analysis of PAK1 in HeLa cells. Samples were treated with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, incubated with polyclonal antibody (Product # PA5-118139) with a dilution of 1:5,000 (4°C overnight), followed by Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and DAPI (blue).
Product Details
PA5-118139
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to the C-terminus of the Rat PAK1
Conjugate
Form
Liquid
Purification
Antigen affinity chromatography, Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2902739
Target Information
PAK1 is a serine/threonine kinase that is a target for restenosis, cancer, and immunosuppression treatment. These proteins serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK1 regulates cell motility and morphology.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ADRB2; alpha pak; Alpha-PAK; EC 2.7.11.1; kinase PAK1; MGC130000; MGC130001; p21 (CDKN1A)-activated kinase 1; p21-activated kinase 1; p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related); p68-PAK; PAK 1; PAK-1; PAK1B; Protein kinase MUK2; Serine/threonine-protein kinase PAK 1
Gene Aliases: Pak1
UniProt ID: (Rat) P35465
Entrez Gene ID: (Rat) 29431
MAPK cascade
activation of MAPKK activity
mitotic cell cycle
response to hypoxia
positive regulation of protein phosphorylation
protein phosphorylation
exocytosis
apoptotic process
small GTPase mediated signal transduction
axonogenesis
neuromuscular junction development
response to organic substance
dendrite development
actin cytoskeleton reorganization
cellular response to insulin stimulus
regulation of actin cytoskeleton organization
positive regulation of peptidyl-serine phosphorylation
positive regulation of intracellular estrogen receptor signaling pathway
intracellular signal transduction
wound healing
receptor clustering
positive regulation of JUN kinase activity
protein autophosphorylation
branching morphogenesis of an epithelial tube
neuron projection morphogenesis
positive regulation of stress fiber assembly
negative regulation of cell proliferation involved in contact inhibition
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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