Proteintech

PARK2/Parkin Monoclonal Antibody (2H5A7)

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View all (39) Parkin antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

66674-1-IG

Applications
Tested Dilution
Publications

Western Blot (WB)

1:2,000-1:8,000
View 18 publications 18 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:250-1:1,000
-

Immunocytochemistry (ICC/IF)

1:50-1:100
View 8 publications 8 publications
Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

2H5A7

Immunogen

PARK2/Parkin Fusion Protein Ag5179 (81-387 aa encoded by BC022014)
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: NATGGDDPR NAAGGCEREP QSLTRVDLSS SVLPGDSVGL AVILHTDSRK DSPPAGSPAG RSIYNSFYVY CKGPCQRVQP GKLRVQCSTC RQATLTLTQG PSCWDDVLIP NRMSGECQSP HCPGTSAEFF FKCGAHPTSD KETSVALHLI ATNSRNITCI TCTDVRSPVL VFQCNSRHVI CLDCFHLYCV TRLNDRQFVH DPQLGYSLPC VGTGDTVVLR GALGGFRRGV AGCPNSLIKE LHHFRILGEE QYNRYQQYGA EECVLQMGGV LCPRPGCGAG LLPEPDQRKV TCEGGNGLGC GYGQRRTK (81-387 aa encoded by BC022014 )

Target Information

The precise function of Parkin gene is unknown; however, the encoded protein is a component of a multiprotein E3 ubiquitin ligase complex that mediates the targeting of substrate proteins for proteasomal degradation. Mutations in this gene are known to cause Parkinson disease and autosomal recessive juvenile Parkinson disease. Alternative splicing of this gene produces multiple transcript variants encoding distinct isoforms. Additional splice variants of this gene have been described but currently lack transcript support.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: E3 ubiquitin ligase; E3 ubiquitin-protein ligase parkin; OTTHUMP00000017562; OTTHUMP00000017563; OTTHUMP00000017564; Parkin; parkin 2; Parkin isoform 1; parkin protein; Parkin RBR E3 ubiquitin-protein ligase; Parkinson disease (autosomal recessive, juvenile) 2, parkin; Parkinson disease protein 2; Parkinson juvenile disease protein 2; parkinson protein 2 E3 ubiquitin protein ligase; parkinson protein 2, E3 ubiquitin protein ligase (parkin)

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Gene Aliases: AR-JP; LPRS2; PARK2; PDJ; PRKN

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UniProt ID: (Human) O60260, (Mouse) Q9WVS6

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Entrez Gene ID: (Human) 5071, (Mouse) 50873

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Function(s)
transcription regulatory region sequence-specific DNA binding G-protein coupled receptor binding transcription factor activity, sequence-specific DNA binding actin binding ubiquitin-protein transferase activity protein binding beta-catenin binding zinc ion binding tubulin binding ligase activity SH3 domain binding enzyme binding kinase binding protein kinase binding PDZ domain binding Hsp70 protein binding heat shock protein binding ubiquitin conjugating enzyme binding ubiquitin protein ligase binding identical protein binding histone deacetylase binding ubiquitin binding phospholipase binding chaperone binding ubiquitin protein ligase activity cullin family protein binding ubiquitin protein ligase activity involved in ERAD pathway ubiquitin-specific protease binding F-box domain binding UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity ribosomal S6-glutamic acid ligase activity protein complex binding coenzyme F420-0 gamma-glutamyl ligase activity coenzyme F420-2 alpha-glutamyl ligase activity metal ion binding protein-glycine ligase activity protein-glycine ligase activity, initiating protein-glycine ligase activity, elongating tubulin-glycine ligase activity protein-glutamic acid ligase activity tubulin-glutamic acid ligase activity ubiquitin-protein ligase
Process(es)
negative regulation of transcription from RNA polymerase II promoter protein polyubiquitination mitochondrial fission mitophagy negative regulation of protein phosphorylation startle response transcription, DNA-templated protein monoubiquitination response to oxidative stress mitochondrion organization central nervous system development learning adult locomotory behavior proteasomal protein catabolic process regulation of autophagy positive regulation of gene expression negative regulation of gene expression positive regulation of mitochondrial fusion negative regulation of mitochondrial fusion regulation of mitochondrion organization regulation of glucose metabolic process free ubiquitin chain polymerization regulation of dopamine secretion macroautophagy protein ubiquitination regulation of protein ubiquitination protein destabilization negative regulation of actin filament bundle assembly regulation of lipid transport positive regulation of proteasomal ubiquitin-dependent protein catabolic process negative regulation of glucokinase activity cellular response to unfolded protein response to endoplasmic reticulum stress synaptic transmission, glutamatergic protein K29-linked ubiquitination ERAD pathway regulation of dopamine metabolic process norepinephrine metabolic process dopamine metabolic process protein ubiquitination involved in ubiquitin-dependent protein catabolic process positive regulation of I-kappaB kinase/NF-kappaB signaling proteasome-mediated ubiquitin-dependent protein catabolic process positive regulation of DNA binding negative regulation of neuron apoptotic process cellular protein catabolic process cellular protein metabolic process protein K27-linked ubiquitination negative regulation by host of viral genome replication positive regulation of protein catabolic process positive regulation of transcription from RNA polymerase II promoter negative regulation of JNK cascade negative regulation of insulin secretion protein stabilization positive regulation of neurotransmitter uptake dopamine uptake involved in synaptic transmission protein autoubiquitination regulation of mitochondrial membrane potential zinc ion homeostasis negative regulation of cell death regulation of canonical Wnt signaling pathway parkin-mediated mitophagy in response to mitochondrial depolarization neuron cellular homeostasis protein K63-linked ubiquitination protein localization to mitochondrion aggresome assembly protein K48-linked ubiquitination protein K11-linked ubiquitination cellular response to manganese ion protein K6-linked ubiquitination negative regulation of canonical Wnt signaling pathway positive regulation of mitochondrial fission negative regulation of release of cytochrome c from mitochondria cellular response to toxic substance mitophagy in response to mitochondrial depolarization regulation of cellular response to oxidative stress negative regulation of neuron death positive regulation of proteasomal protein catabolic process negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway negative regulation of intrinsic apoptotic signaling pathway by p53 class mediator negative regulation of primary amine oxidase activity positive regulation of protein linear polyubiquitination regulation of synaptic vesicle transport negative regulation of oxidative stress-induced cell death regulation of protein targeting to mitochondrion positive regulation of tumor necrosis factor-mediated signaling pathway cellular response to dopamine negative regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway positive regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway negative regulation of endoplasmic reticulum stress-induced neuron intrinsic apoptotic signaling pathway positive regulation of dendrite extension negative regulation of spontaneous neurotransmitter secretion regulation of reactive oxygen species metabolic process negative regulation of reactive oxygen species metabolic process synaptic transmission, dopaminergic regulation of transcription, DNA-templated autophagy response to unfolded protein locomotory behavior protein metabolic process regulation of neurotransmitter secretion modulation of synaptic transmission mitochondrion localization negative regulation of oxidative stress-induced neuron death
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