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PARP1 Antibody (PA5-34802) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of PARP1 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: PARP1 Polyclonal Antibody (Product # PA5-34802) diluted at 1:500. Red: phalloidin, a cytoskeleton marker. Scale bar = 10 µm.
Product Details
PA5-34802
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Published species
Not Applicable
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 34 and 277 of PARP1 (Uniprot ID#P09874)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2552154
Product Specific Information
Recommended positive controls: 293T, A431, HeLa, HepG2, A549, PC-12, Rat2.
Predicted reactivity: Mouse (84%), Rat (85%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Poly ADP-Ribose Polymerase (PARP) uses nicotinamide adenine dinucleotide (oxidized form) NAD as a substrate to catalyse the transfer of ADP-ribose to a variety of nuclear protein acceptors. Proteolysis of PARP to its stable 85kDa fragment is an early marker of programmed cell death (apoptosis) and is mediated by the caspase CPP32 protein. Cleavage occurs between Adp216 and Gly217, a site in PARP conserved across species.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ADP-ribosyltransferase (NAD+; ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase); ADP-ribosyltransferase 1; ADP-ribosyltransferase diphtheria toxin-like 1; ADP-ribosyltransferase NAD(+); ADPRT 1; ARTD1; DNA ADP-ribosyltransferase PARP1; NAD(+) ADP-ribosyltransferase 1; NAD(+) pADPRT-1; PARP-1; poly (ADP-ribose) polymerase 1; poly (ADP-ribose) polymerase family, member 1; poly (ADP-ribose) polymerase); Poly [ADP-ribose] polymerase 1; poly(ADP-ribose) polymerase; poly(ADP-ribose) polymerase PARP-1; poly(ADP-ribose) synthetase; poly(ADP-ribosyl)transferase; Poly[ADP-ribose] synthase 1; poly[ADP-ribose] synthetase 1; Protein poly-ADP-ribosyltransferase PARP1
Gene Aliases: ADPRT; ADPRT 1; ADPRT1; ARTD1; pADPRT-1; PARP; PARP-1; PARP1; PPOL
UniProt ID: (Human) P09874, (Rat) P27008
Entrez Gene ID: (Human) 142, (Rat) 25591
DNA binding
DNA ligase (ATP) activity
NAD+ ADP-ribosyltransferase activity
protein binding
transcription factor binding
zinc ion binding
enzyme binding
protein kinase binding
identical protein binding
histone deacetylase binding
poly(A) RNA binding
protein N-terminus binding
NAD binding
R-SMAD binding
transferase activity, transferring pentosyl groups
DNA metabolism protein
negative regulation of transcription from RNA polymerase II promoter
nucleotide-excision repair, DNA damage recognition
nucleotide-excision repair, DNA duplex unwinding
double-strand break repair via homologous recombination
lagging strand elongation
DNA repair
nucleotide-excision repair, preincision complex stabilization
nucleotide-excision repair, preincision complex assembly
nucleotide-excision repair, DNA incision, 3'-to lesion
nucleotide-excision repair, DNA incision, 5'-to lesion
double-strand break repair
transcription from RNA polymerase II promoter
protein ADP-ribosylation
cellular response to DNA damage stimulus
mitochondrion organization
transforming growth factor beta receptor signaling pathway
positive regulation of cardiac muscle hypertrophy
protein autoprocessing
protein sumoylation
signal transduction involved in regulation of gene expression
macrophage differentiation
mitochondrial DNA metabolic process
cellular response to insulin stimulus
nucleotide-excision repair, DNA incision
cellular response to oxidative stress
protein modification process
DNA damage response, detection of DNA damage
mitochondrial DNA repair
positive regulation of transcription from RNA polymerase II promoter
DNA ligation involved in DNA repair
positive regulation of SMAD protein import into nucleus
protein poly-ADP-ribosylation
global genome nucleotide-excision repair
regulation of cellular protein localization
negative regulation of telomere maintenance via telomere lengthening
ATP generation from poly-ADP-D-ribose
positive regulation of transcription regulatory region DNA binding
telomere maintenance
DNA metabolic process
base-excision repair
transcription, DNA-templated
regulation of growth rate
cellular response to superoxide
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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