Invitrogen

PARP1 Monoclonal Antibody (123)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

9 Published Figures

11 References

View all (69) PARP1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

PARP1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 23

PARP1 Antibody (436400) in ICC/IF

Immunofluorescence analysis of PARP was done on 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with PARP Mouse Monoclonal Antibody (Product # 436400) at 1 µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of... View More

PARP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PARP1 Antibody in Immunoprecipitation (IP)

PARP1 Antibody in RNA Immunoprecipitation (RIP)

Product Image Gallery

PARP1 Antibody — Modulation of expression of target protein by cell treatment to demonstrate antibody specificity. Western blotting analysis of PARP using anti-PARP Monoclonal Antibody (Product # 436400) shows decrease in the proform and corresponding increase in the expression of the cleaved form in Jurkat and HeLa cells treated with Etoposide or Staurosporine. {TM}

Product Details

436400

Applications

Tested Dilution

Publications

Western Blot (WB)

1-3 µg/mL

View 7 publications 7 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:50

Immunocytochemistry (ICC/IF)

1-2 µg/mL

View 1 publication 1 publication

Flow Cytometry (Flow)

Assay-dependent

Immunoprecipitation (IP)

1:100-1:300

View 1 publication 1 publication

RNA Immunoprecipitation (RIP)

Assay-dependent

Miscellaneous PubMed (Misc)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Dog, Horse, Human, Mouse, Rhesus monkey, Rat

Published species

Dog, Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

123

Immunogen

Recombinant protein derived from the C-terminal region of human PARP protein.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2532215

Target Information

Poly ADP-Ribose Polymerase (PARP) uses nicotinamide adenine dinucleotide (oxidized form) NAD as a substrate to catalyse the transfer of ADP-ribose to a variety of nuclear protein acceptors. Proteolysis of PARP to its stable 85kDa fragment is an early marker of programmed cell death (apoptosis) and is mediated by the caspase CPP32 protein. Cleavage occurs between Adp216 and Gly217, a site in PARP conserved across species.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Infigratinib (BGJ 398), a Pan-FGFR Inhibitor, Targets P-Glycoprotein and Increases Chemotherapeutic-Induced Mortality of Multidrug-Resistant Tumor Cells.

Biomedicines

Boichuk S,Dunaev P,Mustafin I,Mani S,Syuzov K,Valeeva E,Bikinieva F,Galembikova A

436400 was used in Western Blotting to show that infigratinib (BGJ 398) reverses the sensitivity of MDR-overexpressing cancer cells to certain chemotherapeutic agents due to inhibition of their efflux from cancer cells via ABCB1-mediated mechanism.

Thu Mar 03 00:00:00 EST 2022

Modulation of Epithelial Mesenchymal Transition after AGTR-1 Gene Edition by Crispr/Cas9 and Losartan Treatment in Mammary Tumor Cell Line: A Comparative Study between Human and Canine Species.

Life (Basel, Switzerland)

Moschetta-Pinheiro MG,Colombo J,Godoy BLV,Balan JF,Nascimento BC,Zuccari DAPC

436400 was used in Immunocytochemistry to study the capacity of losartan and angiotensin II type I receptor (AGTR-1) gene edition to modulate the epithelial-to-mesenchymal transition process in triple negative/metastatic mammary tumor cells, compared to existing treatment protocols such as carboplatin.

Sat Dec 18 00:00:00 EST 2021

Modulation of Epithelial Mesenchymal Transition after AGTR-1 Gene Edition by Crispr/Cas9 and Losartan Treatment in Mammary Tumor Cell Line: A Comparative Study between Human and Canine Species.

Life (Basel, Switzerland)

Moschetta-Pinheiro MG,Colombo J,Godoy BLV,Balan JF,Nascimento BC,Zuccari DAPC

Sat Dec 18 00:00:00 EST 2021

Modulation of Epithelial Mesenchymal Transition after AGTR-1 Gene Edition by Crispr/Cas9 and Losartan Treatment in Mammary Tumor Cell Line: A Comparative Study between Human and Canine Species.

Life (Basel, Switzerland)

Moschetta-Pinheiro MG,Colombo J,Godoy BLV,Balan JF,Nascimento BC,Zuccari DAPC

Sat Dec 18 00:00:00 EST 2021

ERα down-regulates carbohydrate responsive element binding protein and decreases aerobic glycolysis in liver cancer cells.

Journal of cellular and molecular medicine

Lu Y,Tian N,Hu L,Meng J,Feng M,Zhu Y,Zhang P,Li M,Liu Q,Tong L,Tong X,Li Y,Wu L

436400 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, RNA Immunoprecipitation, Western Blot to confirm an original role of ERα in suppressing aerobic glycolysis in liver cancer cells and elucidate the mechanism by which ERα and ChREBP-α together regulate ChREBP-β expression.

Thu Apr 01 00:00:00 EDT 2021

Inhibition of AKT-Signaling Sensitizes Soft Tissue Sarcomas (STS) and Gastrointestinal Stromal Tumors (GIST) to Doxorubicin via Targeting of Homology-Mediated DNA Repair.

International journal of molecular sciences

Boichuk S,Bikinieva F,Nurgatina I,Dunaev P,Valeeva E,Aukhadieva A,Sabirov A,Galembikova A

436400 was used in Western Blotting to study the role of AKT signaling in regulating of Rad51 turnover and cytotoxic effects of topoisomerase II inhibitor, doxorubicin (Dox) in soft tissue sarcomas (STS) and gastrointestinal stromal tumors (GIST) in vitro.

Sun Nov 22 00:00:00 EST 2020

Inhibition of AKT-Signaling Sensitizes Soft Tissue Sarcomas (STS) and Gastrointestinal Stromal Tumors (GIST) to Doxorubicin via Targeting of Homology-Mediated DNA Repair.

International journal of molecular sciences

Boichuk S,Bikinieva F,Nurgatina I,Dunaev P,Valeeva E,Aukhadieva A,Sabirov A,Galembikova A

Sun Nov 22 00:00:00 EST 2020

Proteomics Profiling of KAIMRC1 in Comparison to MDA-MB231 and MCF-7.

International journal of molecular sciences

Alghanem B,Ali R,Nehdi A,Al Zahrani H,Altolayyan A,Shaibah H,Baz O,Alhallaj A,Moresco JJ,Diedrich JK,Yates JR,Boudjelal M

436400 was used in Western Blotting to perform a Tandem Mass Tag (TMT) strategy for accurate peptide/protein quantification.

Thu Jun 18 00:00:00 EDT 2020

Proteomics Profiling of KAIMRC1 in Comparison to MDA-MB231 and MCF-7.

International journal of molecular sciences

Alghanem B,Ali R,Nehdi A,Al Zahrani H,Altolayyan A,Shaibah H,Baz O,Alhallaj A,Moresco JJ,Diedrich JK,Yates JR,Boudjelal M

436400 was used in Western Blotting to perform a Tandem Mass Tag (TMT) strategy for accurate peptide/protein quantification.

Thu Jun 18 00:00:00 EDT 2020

Establishment and characterization of a triple negative basal-like breast cancer cell line with multi-drug resistance.

Oncology letters

Boichuk S,Galembikova A,Sitenkov A,Khusnutdinov R,Dunaev P,Valeeva E,Usolova N

Published figure using PARP monoclonal antibody (Product # 436400) in Western Blot

Sun Oct 01 00:00:00 EDT 2017

USP17-mediated deubiquitination and stabilization of HDAC2 in cigarette smoke extract-induced inflammation.

International journal of clinical and experimental pathology

Song H,Tao L,Chen C,Pan L,Hao J,Ni Y,Li D,Li B,Shi G

Published figure using PARP1 monoclonal antibody (Product # 436400) in Western Blot

Mon Sep 26 00:00:00 EDT 2016

TIMELESS Forms a Complex with PARP1 Distinct from Its Complex with TIPIN and Plays a Role in the DNA Damage Response.

Cell reports

Young LM,Marzio A,Perez-Duran P,Reid DA,Meredith DN,Roberti D,Star A,Rothenberg E,Ueberheide B,Pagano M

Published figure using PARP monoclonal antibody (Product # 436400) in Western Blot

Tue Oct 20 00:00:00 EDT 2015

TIMELESS Forms a Complex with PARP1 Distinct from Its Complex with TIPIN and Plays a Role in the DNA Damage Response.

Cell reports

Young LM,Marzio A,Perez-Duran P,Reid DA,Meredith DN,Roberti D,Star A,Rothenberg E,Ueberheide B,Pagano M

Published figure using PARP monoclonal antibody (Product # 436400) in Western Blot

Tue Oct 20 00:00:00 EDT 2015

The Deubiquitinase USP17 Regulates the Stability and Nuclear Function of IL-33.

International journal of molecular sciences

Ni Y,Tao L,Chen C,Song H,Li Z,Gao Y,Nie J,Piccioni M,Shi G,Li B

436400 was used in western blot to investigate how IL-33 regulates IL-13 gene expression

Tue Nov 24 00:00:00 EST 2015

Deubiquitination and stabilization of IL-33 by USP21.

International journal of clinical and experimental pathology

Tao L,Chen C,Song H,Piccioni M,Shi G,Li B

436400 was used in immunoprecipitation to study the effect of ubiquitination modification in regulating the protein stability and the nuclear function of IL-33

Tue Jun 09 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: ADP-ribosyltransferase (NAD+; ADP-ribosyltransferase (NAD+, poly (ADP-ribose) polymerase) 1; ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase); ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase) 1; ADP-ribosyltransferase 1; ADP-ribosyltransferase diphtheria toxin-like 1; ADP-ribosyltransferase NAD(+); ADPRT 1; ARTD1; DNA ADP-ribosyltransferase PARP1; msPARP; NAD(+) ADP-ribosyltransferase 1; NAD(+) pADPRT-1; PARP-1; poly (ADP-ribose) polymerase 1; poly (ADP-ribose) polymerase family, member 1; poly (ADP-ribose) polymerase); Poly [ADP-ribose] polymerase 1; poly(ADP-ribose) polymerase; poly(ADP-ribose) polymerase PARP-1; poly(ADP-ribose) synthetase; poly(ADP-ribosyl)transferase; Poly[ADP-ribose] synthase 1; poly[ADP-ribose] synthetase 1; Protein poly-ADP-ribosyltransferase PARP1

View more View less

Gene Aliases: 5830444G22Rik; Adprp; ADPRT; ADPRT 1; ADPRT1; AI893648; ARTD1; C80510; pADPRT-1; PARP; PARP-1; PARP1; PPOL; sPARP-1

View more View less

UniProt ID: (Human) P09874, (Rat) P27008

View more View less

Entrez Gene ID: (Dog) 490385, (Human) 142, (Horse) 100058523, (Rhesus monkey) 698806, (Rat) 25591, (Mouse) 11545

View more View less

Function(s)

DNA binding DNA ligase (ATP) activity NAD+ ADP-ribosyltransferase activity protein binding transcription factor binding zinc ion binding enzyme binding protein kinase binding identical protein binding histone deacetylase binding poly(A) RNA binding protein N-terminus binding NAD binding R-SMAD binding transferase activity, transferring pentosyl groups chromatin binding transferase activity transferase activity, transferring glycosyl groups metal ion binding DNA metabolism protein

Process(es)

negative regulation of transcription from RNA polymerase II promoter nucleotide-excision repair, DNA damage recognition nucleotide-excision repair, DNA duplex unwinding double-strand break repair via homologous recombination lagging strand elongation DNA repair nucleotide-excision repair, preincision complex stabilization nucleotide-excision repair, preincision complex assembly nucleotide-excision repair, DNA incision, 3'-to lesion nucleotide-excision repair, DNA incision, 5'-to lesion double-strand break repair transcription from RNA polymerase II promoter protein ADP-ribosylation cellular response to DNA damage stimulus mitochondrion organization transforming growth factor beta receptor signaling pathway positive regulation of cardiac muscle hypertrophy protein autoprocessing protein sumoylation signal transduction involved in regulation of gene expression macrophage differentiation mitochondrial DNA metabolic process cellular response to insulin stimulus nucleotide-excision repair, DNA incision cellular response to oxidative stress protein modification process DNA damage response, detection of DNA damage mitochondrial DNA repair positive regulation of transcription from RNA polymerase II promoter DNA ligation involved in DNA repair positive regulation of SMAD protein import into nucleus protein poly-ADP-ribosylation global genome nucleotide-excision repair regulation of cellular protein localization negative regulation of telomere maintenance via telomere lengthening ATP generation from poly-ADP-D-ribose positive regulation of transcription regulatory region DNA binding telomere maintenance DNA metabolic process base-excision repair transcription, DNA-templated regulation of growth rate cellular response to superoxide regulation of transcription, DNA-templated behavioral response to cocaine regulation of single strand break repair

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

PARP1 Monoclonal Antibody (123)

PARP1 Antibody (436400) in ICC/IF

Product Details

436400

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

RNA Immunoprecipitation (RIP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!