Invitrogen

PARP1 Recombinant Rabbit Monoclonal Antibody (8H2L9)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

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Datasheet
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Datasheet
Protocols
Questions & Answers

Product Details

702343

Applications
Tested Dilution
Publications

Western Blot (WB)

1-2 µg/mL
-

Immunocytochemistry (ICC/IF)

2 µg/mL
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

8H2L9

Immunogen

Peptide corresponding to Human PARP1 (aa 215-225)
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2633085

Product Specific Information

This antibody is predicted to react with Monkey, Pig and Rabbit

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

Poly ADP-Ribose Polymerase (PARP) uses nicotinamide adenine dinucleotide (oxidized form) NAD as a substrate to catalyse the transfer of ADP-ribose to a variety of nuclear protein acceptors. Proteolysis of PARP to its stable 85kDa fragment is an early marker of programmed cell death (apoptosis) and is mediated by the caspase CPP32 protein. Cleavage occurs between Adp216 and Gly217, a site in PARP conserved across species.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase); ADP-ribosyltransferase diphtheria toxin-like 1; ADP-ribosyltransferase NAD(+); ADPRT 1; ARTD1; DNA ADP-ribosyltransferase PARP1; NAD(+) ADP-ribosyltransferase 1; NAD(+) pADPRT-1; PARP-1; poly (ADP-ribose) polymerase 1; poly (ADP-ribose) polymerase family, member 1; Poly [ADP-ribose] polymerase 1; poly(ADP-ribose) polymerase; poly(ADP-ribose) synthetase; poly(ADP-ribosyl)transferase; Poly[ADP-ribose] synthase 1; Protein poly-ADP-ribosyltransferase PARP1

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Gene Aliases: ADPRT; ADPRT 1; ADPRT1; ARTD1; pADPRT-1; PARP; PARP-1; PARP1; PPOL

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UniProt ID: (Human) P09874

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Entrez Gene ID: (Human) 142

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Function(s)
DNA binding DNA ligase (ATP) activity NAD+ ADP-ribosyltransferase activity protein binding transcription factor binding zinc ion binding enzyme binding protein kinase binding identical protein binding histone deacetylase binding poly(A) RNA binding protein N-terminus binding NAD binding R-SMAD binding DNA metabolism protein
Process(es)
negative regulation of transcription from RNA polymerase II promoter nucleotide-excision repair, DNA damage recognition nucleotide-excision repair, DNA duplex unwinding double-strand break repair via homologous recombination lagging strand elongation DNA repair nucleotide-excision repair, preincision complex stabilization nucleotide-excision repair, preincision complex assembly nucleotide-excision repair, DNA incision, 3'-to lesion nucleotide-excision repair, DNA incision, 5'-to lesion double-strand break repair transcription from RNA polymerase II promoter protein ADP-ribosylation cellular response to DNA damage stimulus mitochondrion organization transforming growth factor beta receptor signaling pathway positive regulation of cardiac muscle hypertrophy protein autoprocessing protein sumoylation signal transduction involved in regulation of gene expression macrophage differentiation mitochondrial DNA metabolic process cellular response to insulin stimulus nucleotide-excision repair, DNA incision cellular response to oxidative stress protein modification process DNA damage response, detection of DNA damage mitochondrial DNA repair positive regulation of transcription from RNA polymerase II promoter DNA ligation involved in DNA repair positive regulation of SMAD protein import into nucleus protein poly-ADP-ribosylation global genome nucleotide-excision repair regulation of cellular protein localization negative regulation of telomere maintenance via telomere lengthening ATP generation from poly-ADP-D-ribose positive regulation of transcription regulatory region DNA binding
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