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FIGURE: 1 / 1
PATJ Antibody (PA5-97072) in WB
Western blot analysis of PATJ in extracts of various cell lines. Samples were incubated with PATJ Polyclonal antibody (Product # PA5-97072) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Product Details
PA5-97072
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 450-680 of human INADL (NP_7953522)
Conjugate
Form
Liquid
Concentration
1.86 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2808874
Product Specific Information
Positive Samples: SKOV3, SW480, 293T, HeLa; Cellular Location: Apical cell membrane, Cell junction, Cell membrane, Cytoplasm, Peripheral membrane protein, perinuclear region, tight junction
Immunogen sequence: VRRKTSSSTS PLEPPSDRGT VVEPLKPPAL FLTGAVETET NVDGEDEEIK ERIDTLKNDN IQALEKLEKV PDSPENELKS RWENLLGPDY EVMVATLDTQ IADDAELQKY SKLLPIHTLR LGVEVDSFDG HHYISSIVSG GPVDTLGLLQ PEDELLEVNG MQLYGKSRRE AVSFLKEVPP PFTLVCCRRL FDDEASVDEP RRTETSLPET EVDHNMDVNT EEDDDGELAL W
Target Information
This gene encodes a protein with multiple PDZ domains. PDZ domains mediate protein-protein interactions, and proteins with multiple PDZ domains often organize multimeric complexes at the plasma membrane. This protein localizes to tight junctions and to the apical membrane of epithelial cells. A similar protein in Drosophila is a scaffolding protein which tethers several members of a multimeric signaling complex in photoreceptors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: channel-interacting PDZ domain protein; Channel-interacting PDZ domain-containing protein; inactivation no after-potential D-like protein; inactivation-no-afterpotential D-like; InaD-like protein; Inadl protein; novel ankyrin-repeat protein; PALS1-associated tight junction protein; PDZ domain protein; Protein associated to tight junctions
Gene Aliases: Cipp; hINADL; InaD-like; INADL; Patj
UniProt ID: (Mouse) Q63ZW7
Entrez Gene ID: (Mouse) 12695, (Human) 10207
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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