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PAX3 Antibody (PA5-85400) in WB
Western blot was performed using Anti-PAX3 Polyclonal Antibody (Product # PA5-85400) and a 55 kDa band corresponding to PAX3 was observed in positive cell line (U-87 MG) and not across negative cell lines (T-47D, MCF7) along with an uncharacterized band across cell lines tested. Nuclear enriched extracts (30 µg lysate) of U-87 MG (Lane 1), T-47D (Lane 2), MCF7 (Lane 3) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001)... View More
Product Details
PA5-85400
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human PAX3.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 1% BSA, 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2792542
Product Specific Information
Keep as concentrated solution.
Predicted reactivity: Mouse (95%), Rat (95%), Xenopus laevis (90%), Chicken (97%), Bovine (98%).
Positive Control: PAX3-transfected 293T.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Pax 3 (Paired Box 3) is a transcription factor that is involved in development of the peripheral nervous system, melanocytes, some vascular smooth muscle, and a number of other derivatives. It regulates neurogenesis in pre-migratory neural crest cells from the dorsal neural tube, and in myogenic progenitors in the presomitic mesoderm and the hypaxial somites. Alternative splicing results in isoforms having different C-termini.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: HuP2; MGC120381; MGC120382; MGC120383; MGC120384; MGC134778; paired box homeotic gene 3; Paired box protein Pax-3; paired domain gene 3; paired domain gene HuP2
Gene Aliases: CDHS; HUP2; PAX3; WS1; WS3
UniProt ID: (Human) P23760
Entrez Gene ID: (Human) 5077
transcription from RNA polymerase II promoter
apoptotic process
nervous system development
muscle organ development
sensory perception of sound
organ morphogenesis
neural crest formation
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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