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Invitrogen
PAX3 Recombinant Rabbit Monoclonal Antibody (16H22L10)
FIGURE: 1 / 5
PAX3 Antibody (701147) in ICC/IF
Immunofluorescent analysis of PAX3 in U2OS cells using a PAX3 recombinant rabbit monoclonal antibody (Product # 701147) followed by detection using an Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody (green) (Image A). Nuclei were stained using DAPI (Image B) and actin stained with Alexa Fluor 594 phalloidin (red) (image C). Image D is a composite image showing nuclear localization of Pax 3.
Product Details
701147
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
16H22L10
Immunogen
Recombinant protein corresponding to amino acids 34-276 of human Pax 3
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2532409
Product Specific Information
This antibody is predicted to react with mouse based on sequence homology.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
Pax 3 (Paired Box 3) is a transcription factor that is involved in development of the peripheral nervous system, melanocytes, some vascular smooth muscle, and a number of other derivatives. It regulates neurogenesis in pre-migratory neural crest cells from the dorsal neural tube, and in myogenic progenitors in the presomitic mesoderm and the hypaxial somites. Alternative splicing results in isoforms having different C-termini.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: HuP2; MGC120381; MGC120382; MGC120383; MGC120384; MGC134778; paired box gene 3; paired box homeotic gene 3; Paired box protein Pax-3; paired domain gene 3; paired domain gene HuP2; PAX3/FKHR fusion
Gene Aliases: CDHS; HUP2; Pax-3; PAX3; Sp; splotch; WS1; WS3
UniProt ID: (Human) P23760, (Mouse) P24610
Entrez Gene ID: (Human) 5077, (Mouse) 18505
transcription from RNA polymerase II promoter
apoptotic process
nervous system development
muscle organ development
sensory perception of sound
organ morphogenesis
neural crest formation
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
negative regulation of transcription from RNA polymerase II promoter
neural crest cell migration
neural tube closure
transcription, DNA-templated
regulation of transcription, DNA-templated
multicellular organism development
heart development
cell proliferation
positive regulation of cell proliferation
regulation of somitogenesis
cell migration
spinal cord association neuron differentiation
neural tube development
skeletal muscle cell differentiation
developmental pigmentation
neuron fate commitment
cardiac muscle cell differentiation
skeletal muscle organ development
mammary gland specification
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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