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PAX5 Antibody (MA5-36223) in ICC/IF
Immunocytochemistry analysis of PAX5 in U20S cell. Antigen retrieval was performed with enzyme antigen retrieval (15 min). Samples were blocked with 10% goat serum and incubated in PAX5 monoclonal antibody (Product # MA5-36223) at a dilution of 2 µg/mL (overnight, 4°C), followed by DyLight 488 conjugated goat anti-mouse IgG (30 min, 37°C) and DAPI at a dilution of 1:100.
Product Details
MA5-36223
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
6I4
Immunogen
E. coli-derived human PAX5 recombinant protein (Position: R217-A282).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2884057
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Target Information
PAX5 is a member of the paired box (PAX) family of transcription factors. The PAX proteins are important regulators in early development and alterations in the expression of their genes are thought to contribute to neoplastic transformation. PAX5 is the B-cell lineage specific activator protein (BSAP) that is expressed at early, but not late stages, of B-cell differentiation. Its expression has also been detected in developing CNS and testis, therefore, PAX5 may not only play an important role in B-cell differentiation, but also in neural development and spermatogenesis. Mutations in the gene can result in leukemia, acute lymphoblastic 3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: B-cell lineage specific activator; B-cell-specific transcription factor; BSAP; paired box gene 5; paired box homeotic gene 5; Paired box protein Pax-5; paired domain gene 5; Pax5 variant 1; Pax5 variant 2; transcription factor PAX 5
Gene Aliases: ALL3; BSAP; EBB-1; KLP; Pax-5; PAX5; RGD1565236
UniProt ID: (Human) Q02548, (Mouse) Q02650
Entrez Gene ID: (Human) 5079, (Rat) 500453, (Mouse) 18507
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
skeletal muscle cell differentiation
positive regulation of transcription from RNA polymerase II promoter
negative regulation of histone H3-K9 methylation
multicellular organism development
spermatogenesis
nervous system development
aging
organ morphogenesis
lateral ventricle development
cerebral cortex development
cell differentiation
adult behavior
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
embryonic cranial skeleton morphogenesis
humoral immune response
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