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Invitrogen
PAX5 Monoclonal Antibody (7D3)
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 7
PAX5 Antibody (MA5-17143) in ICC/IF
Immunofluorescence analysis of PAX5 was performed using 70% confluent log phase Ramos cells. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with PAX5 Monoclonal Antibody (7D3) (Product # MA5-17143) at 1 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor 488 (Product # A28175) at... View More
Product Details
MA5-17143
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
7D3
Immunogen
Purified recombinant fragment of human PAX5 (amino acids: 235-382) expressed in E. Coli.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2538614
Product Specific Information
MA5-17143 targets PAX5 in indirect ELISA, IHC and WB applications and shows reactivity with Human samples.
The MA5-17143 immunogen is purified recombinant fragment of human PAX5 (amino acids: 235-382) expressed in E. Coli.
MA5-17143 detects PAX5 which has a predicted molecular weight of approximately 45kDa.
Target Information
PAX5 is a member of the paired box (PAX) family of transcription factors. The PAX proteins are important regulators in early development and alterations in the expression of their genes are thought to contribute to neoplastic transformation. PAX5 is the B-cell lineage specific activator protein (BSAP) that is expressed at early, but not late stages, of B-cell differentiation. Its expression has also been detected in developing CNS and testis, therefore, PAX5 may not only play an important role in B-cell differentiation, but also in neural development and spermatogenesis. Mutations in the gene can result in leukemia, acute lymphoblastic 3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: B-cell lineage specific activator; B-cell-specific transcription factor; BSAP; paired box homeotic gene 5; Paired box protein Pax-5; paired domain gene 5; transcription factor PAX 5
Gene Aliases: ALL3; BSAP; PAX5
UniProt ID: (Human) Q02548
Entrez Gene ID: (Human) 5079
negative regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
humoral immune response
multicellular organism development
spermatogenesis
aging
organ morphogenesis
lateral ventricle development
cerebral cortex development
adult behavior
skeletal muscle cell differentiation
positive regulation of transcription from RNA polymerase II promoter
embryonic cranial skeleton morphogenesis
negative regulation of histone H3-K9 methylation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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