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Invitrogen
PAX5 Monoclonal Antibody (SP34)
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 8
PAX5 Antibody (MA5-14585) in ICC/IF
Immunofluorescence analysis of PAX5 was performed using 70% confluent log phase Ramos cells. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with PAX5 Monoclonal Antibody (SP34) (Product # MA5-14585) at 1:250 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor 488 (Pro... View More
Product Details
MA5-14585
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Monoclonal
Type
Antibody
Clone
SP34
Immunogen
Synthetic peptide derived from the C-terminus of human Pax-5 protein
Conjugate
Form
Liquid
Concentration
0.067 mg/mL
Purification
Protein A/G
Storage buffer
PBS, pH 7.6, with 1% BSA
Contains
0.1% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_10980054
Product Specific Information
MA5-14585 targets Pax-5 in IHC (P) applications and shows reactivity with Human and Mouse samples.
The MA5-14585 immunogen is synthetic peptide derived from the C-terminus of human Pax-5 protein.
Target Information
PAX5 is a member of the paired box (PAX) family of transcription factors. The PAX proteins are important regulators in early development and alterations in the expression of their genes are thought to contribute to neoplastic transformation. PAX5 is the B-cell lineage specific activator protein (BSAP) that is expressed at early, but not late stages, of B-cell differentiation. Its expression has also been detected in developing CNS and testis, therefore, PAX5 may not only play an important role in B-cell differentiation, but also in neural development and spermatogenesis. Mutations in the gene can result in leukemia, acute lymphoblastic 3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: B-cell lineage specific activator; B-cell-specific transcription factor; BSAP; paired box homeotic gene 5; Paired box protein Pax-5; paired domain gene 5; transcription factor PAX 5
Gene Aliases: ALL3; BSAP; PAX5; RGD1565236
UniProt ID: (Human) Q02548
Entrez Gene ID: (Human) 5079, (Rat) 500453
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
skeletal muscle cell differentiation
positive regulation of transcription from RNA polymerase II promoter
negative regulation of histone H3-K9 methylation
humoral immune response
multicellular organism development
spermatogenesis
aging
organ morphogenesis
lateral ventricle development
cerebral cortex development
adult behavior
embryonic cranial skeleton morphogenesis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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