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PAX6 Antibody (MA5-50174) in IHC (P)
Immunohistochemistry analysis with a PAX6 monoclonal antibody (Product # MA5-50174) using a dilution of 1:100 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 min at RT. Then primary antibody (1% BSA) was in... View More
Product Details
MA5-50174
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
22C3
Immunogen
A synthesized peptide derived from Human PAX6
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Wet ice
RRID
AB_3092549
Target Information
This gene encodes paired box gene 6, one of many human homologs of the Drosophila melanogaster gene prd. In addition to the hallmark feature of this gene family, a conserved paired box domain, the encoded protein also contains a homeo box domain. Both domains are known to bind DNA, and function as regulators of gene transcription. This gene is expressed in the developing nervous system, and in developing eyes. Mutations in this gene are known to cause ocular disorders such as aniridia and Peter's anomaly. Alternatively spliced transcript variants encoding either the same or different isoform have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Aniridia; Aniridia type II protein; Keratitis; MGC17209; Oculorhombin; Paired Box Gene-6; paired box homeotic gene-6; Paired box protein Pax-6
Gene Aliases: AN; AN2; D11S812E; FVH1; MGDA; PAX6; WAGR
UniProt ID: (Human) P26367
Entrez Gene ID: (Human) 5080
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II core promoter sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
ubiquitin-protein transferase activity
protein binding
transcription factor binding
protein kinase binding
ubiquitin protein ligase binding
histone acetyltransferase binding
co-SMAD binding
R-SMAD binding
HMG box domain binding
negative regulation of transcription from RNA polymerase II promoter
establishment of mitotic spindle orientation
blood vessel development
eye development
cell fate determination
neuron migration
negative regulation of protein phosphorylation
positive regulation of neuroblast proliferation
lens development in camera-type eye
type B pancreatic cell differentiation
pancreatic A cell development
transcription from RNA polymerase II promoter
smoothened signaling pathway
axon guidance
central nervous system development
salivary gland morphogenesis
visual perception
response to wounding
regulation of asymmetric cell division
organ morphogenesis
dorsal/ventral axis specification
positive regulation of gene expression
protein ubiquitination
oligodendrocyte cell fate specification
cerebral cortex regionalization
forebrain anterior/posterior pattern specification
forebrain dorsal/ventral pattern formation
commitment of neuronal cell to specific neuron type in forebrain
forebrain-midbrain boundary formation
regulation of transcription from RNA polymerase II promoter involved in spinal cord motor neuron fate specification
regulation of transcription from RNA polymerase II promoter involved in ventral spinal cord interneuron specification
regulation of transcription from RNA polymerase II promoter involved in somatic motor neuron fate commitment
pituitary gland development
signal transduction involved in regulation of gene expression
keratinocyte differentiation
regulation of cell migration
positive regulation of epithelial cell differentiation
lacrimal gland development
protein localization to organelle
eye photoreceptor cell development
glucose homeostasis
negative regulation of neuron differentiation
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
regulation of timing of cell differentiation
embryonic camera-type eye morphogenesis
neuron fate commitment
astrocyte differentiation
negative regulation of epithelial cell proliferation
negative regulation of neurogenesis
retina development in camera-type eye
iris morphogenesis
cornea development in camera-type eye
negative regulation of neural precursor cell proliferation
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