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Invitrogen
PAX8 Polyclonal Antibody
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 12
PAX8 Antibody (PA5-21368) in ICC/IF
Immunofluorescence analysis of PAX8 was performed using 70% confluent log phase SK-OV-3 cells. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with PAX8 Polyclonal Antibody (Product # PA5-21368) at 1:200 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor 488 (Product ... View More
Product Details
PA5-21368
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 204 (Q06710) of PAX8C
Conjugate
Form
Liquid
Concentration
1.84 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_11151941
Product Specific Information
Recommended positive controls: 293T, 293T nuclear extract, DDDDK-tagged PAX8-transfected 293T.
Predicted reactivity: Mouse (98%), Rat (99%), Xenopus laevis (91%), Dog (99%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
This gene encodes a member of the paired box family of transcription factors. Members of this gene family typically encode proteins that contain a paired box domain, an octapeptide, and a paired-type homeodomain. This nuclear protein is involved in thyroid follicular cell development and expression of thyroid-specific genes. Mutations in this gene have been associated with thyroid dysgenesis, thyroid follicular carcinomas and atypical follicular thyroid adenomas. Alternatively spliced transcript variants encoding different isoforms have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: HGNC:8622; paired box gene 8; Paired box protein Pax-8; paired domain gene 8; Pax-8 protein
Gene Aliases: Pax-8; PAX8
UniProt ID: (Human) Q06710, (Mouse) Q00288, (Rat) P51974
Entrez Gene ID: (Human) 7849, (Mouse) 18510, (Rat) 81819
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II core promoter sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
thyroid-stimulating hormone receptor activity
protein binding
transcription regulatory region DNA binding
sequence-specific DNA binding
urogenital system development
metanephros development
mesonephros development
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
multicellular organism development
organ morphogenesis
cell differentiation
thyroid gland development
inner ear morphogenesis
regulation of apoptotic process
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
pronephros development
cellular response to gonadotropin stimulus
positive regulation of mesenchymal to epithelial transition involved in metanephros morphogenesis
metanephric distal convoluted tubule development
metanephric nephron tubule formation
negative regulation of mesenchymal cell apoptotic process involved in metanephric nephron morphogenesis
regulation of metanephric nephron tubule epithelial cell differentiation
positive regulation of branching involved in ureteric bud morphogenesis
negative regulation of mesenchymal cell apoptotic process involved in metanephros development
negative regulation of apoptotic process involved in metanephric collecting duct development
negative regulation of apoptotic process involved in metanephric nephron tubule development
positive regulation of metanephric DCT cell differentiation
positive regulation of thyroid hormone generation
regulation of thyroid-stimulating hormone secretion
branching involved in ureteric bud morphogenesis
kidney development
mesenchymal to epithelial transition involved in metanephros morphogenesis
sulfur compound metabolic process
central nervous system development
anatomical structure morphogenesis
thyroid-stimulating hormone signaling pathway
pronephric field specification
otic vesicle development
metanephric epithelium development
metanephric comma-shaped body morphogenesis
metanephric S-shaped body morphogenesis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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