Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
PAX8 Monoclonal Antibody (1F8-3A8)
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 15
PAX8 Antibody (MA1-117) in ICC/IF
Immunofluorescent analysis of PAX8 (green) in 293T (left panel) and COS7 cells (right panel). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a PAX8 monoclonal antibody (Product # MA1-117) at a dilution of 1:50 for at least 1 hour at room temperature, washed with PBS, and incubated with a DyLight 488-conjugated goat anti-mouse IgG secondary antibody (Product # 35502) a... View More
Product Details
MA1-117
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate
Published species
Human,
Mouse
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
1F8-3A8
Immunogen
Purified internal fragment of human recombinant PAX8 expressed in E. coli.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS with 1mg/mL BSA, 30% glycerol
Contains
0.05% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2536828
Product Specific Information
Western blot analysis of MA1-117 detects a prominent ~48 kDa protein in human adenocarcinoma SKOV-3 and SV40 transformed African green monkey (Cercopithecus aethiops) kidney cells. In SKOV-3 cells, additional unknown low molecular bands are also detected. Specificity of the antibody was confirmed in HeLa (negative control) and 293T cells overexpressing full length PAX8.
Target Information
This gene encodes a member of the paired box family of transcription factors. Members of this gene family typically encode proteins that contain a paired box domain, an octapeptide, and a paired-type homeodomain. This nuclear protein is involved in thyroid follicular cell development and expression of thyroid-specific genes. Mutations in this gene have been associated with thyroid dysgenesis, thyroid follicular carcinomas and atypical follicular thyroid adenomas. Alternatively spliced transcript variants encoding different isoforms have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: HGNC:8622; paired box gene 8; Paired box protein Pax-8; paired domain gene 8
Gene Aliases: Pax-8; PAX8
UniProt ID: (Human) Q06710, (Mouse) Q00288
Entrez Gene ID: (Human) 7849, (Mouse) 18510
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II core promoter sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
thyroid-stimulating hormone receptor activity
protein binding
transcription regulatory region DNA binding
sequence-specific DNA binding
urogenital system development
metanephros development
mesonephros development
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
multicellular organism development
organ morphogenesis
cell differentiation
thyroid gland development
inner ear morphogenesis
regulation of apoptotic process
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
pronephros development
cellular response to gonadotropin stimulus
positive regulation of mesenchymal to epithelial transition involved in metanephros morphogenesis
metanephric distal convoluted tubule development
metanephric nephron tubule formation
negative regulation of mesenchymal cell apoptotic process involved in metanephric nephron morphogenesis
regulation of metanephric nephron tubule epithelial cell differentiation
positive regulation of branching involved in ureteric bud morphogenesis
negative regulation of mesenchymal cell apoptotic process involved in metanephros development
negative regulation of apoptotic process involved in metanephric collecting duct development
negative regulation of apoptotic process involved in metanephric nephron tubule development
positive regulation of metanephric DCT cell differentiation
positive regulation of thyroid hormone generation
regulation of thyroid-stimulating hormone secretion
branching involved in ureteric bud morphogenesis
kidney development
mesenchymal to epithelial transition involved in metanephros morphogenesis
sulfur compound metabolic process
central nervous system development
anatomical structure morphogenesis
thyroid-stimulating hormone signaling pathway
pronephric field specification
otic vesicle development
metanephric epithelium development
metanephric comma-shaped body morphogenesis
metanephric S-shaped body morphogenesis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support