Proteintech

PCK1 Polyclonal Antibody

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Antibody Testing Data (9)

PCK1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 9

PCK1 Antibody (16754-1-AP) in ICC/IF

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HepG2 cells using 16754-1-AP (PCK1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

PCK1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PCK1 Antibody in Immunoprecipitation (IP)

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Product Details

16754-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

View 48 publications 48 publications

Immunohistochemistry (IHC)

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:400

Immunocytochemistry (ICC/IF)

1:20-1:200

View 8 publications 8 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 3 publications 3 publications

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Goat, Human, Mouse, Pig, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

PCK1 Fusion Protein Ag10261 (274-622 aa encoded by BC023978)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.27 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: EGEKKYLAA AFPSACGKTN LAMMNPSLPG WKVECVGDDI AWMKFDAQGH LRAINPENGF FGVAPGTSVK TNPNAIKTIQ KNTIFTNVAE TSDGGVYWEG IDEPLASGVT ITSWKNKEWS SEDGEPCAHP NSRFCTPASQ CPIIDAAWES PEGVPIEGII FGGRRPAGVP LVYEALSWQH GVFVGAAMRS EATAAAEHKG KIIMHDPFAM RPFFGYNFGK YLAHWLSMAQ HPAAKLPKIF HVNWFRKDKE GKFLWPGFGE NSRVLEWMFN RIDGKASTKL TPIGYIPKED ALNLKGLGHI NMMELFSISK EFWEKEVEDI EKYLEDQVNA DLPCEIEREI LALKQRISQM (274-622 aa encoded by BC023978)

Target Information

Phosphoenolpyruvate Carboxykinase 1 (PCK1) is a main control point for the regulation of gluconeogenesis. During gluconeogenesis PCK1 acts as the rate-limiting enzyme. PCK1 regulates the formation and maintenance of memory CH8(+) T-cells via gluconeogenesis. PCK1 aids in controlling the levels of metabolic intermediates in the citric acid cycle by regulating cataplerosis and anaplerosis. When glucose levels are high, PCK1 catalyzes the cataplerotic conversion of oxaloacetate to phosphoenolpyruvate (PEP). When they are low it catalyzes the anaplerotic conversion of phosphoenolpyruvate to oxaloacetate. When phosphorylated at Ser-90 by AKT1, PCK1 acts as a protein kinase by reducing the binding affinity to oxaloacetate and promotes an atypical serine protein kinase activity using GTP as the donor. This activity regulates lipogenesis by disrupting the interaction between INSIG proteins and SCAP and promoting nuclear translocation of SREBP proteins and transcription of lipogenesis-related genes. An important paralog of this gene is PCK2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Tue Oct 01 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: HGNC:8724; MGC22652; PE PEPCKC; PEP carboxykinase; PEPCK-C; phosphoenolpyruvate carboxykinase (GTP); phosphoenolpyruvate carboxykinase 1 (soluble); phosphoenolpyruvate carboxykinase 1, cytosolic; phosphoenolpyruvate carboxykinase, cytosolic; phosphoenolpyruvate carboxykinase, cytosolic (GTP); Phosphoenolpyruvate carboxykinase, cytosolic [GTP]; phosphoenolpyruvate carboxylase; phosphopyruvate carboxylase; Serine-protein kinase PCK1

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Gene Aliases: AI265463; GTP; PCK; Pck-1; PCK1; PEPCK; PEPCK-C; PEPCK1; PEPCKC; RATPEPCK

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UniProt ID: (Human) P35558, (Rat) P07379, (Mouse) Q9Z2V4

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Entrez Gene ID: (Human) 5105, (Rat) 362282, (Mouse) 18534

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Function(s)

magnesium ion binding phosphoenolpyruvate carboxykinase (GTP) activity GTP binding GDP binding manganese ion binding carboxylic acid binding phosphoenolpyruvate carboxykinase activity nucleotide binding lyase activity carboxy-lyase activity purine nucleotide binding metal ion binding kinase

Process(es)

glucose metabolic process gluconeogenesis oxaloacetate metabolic process internal protein amino acid acetylation response to activity response to insulin glucose homeostasis glycerol biosynthetic process from pyruvate cellular response to potassium ion starvation positive regulation of transcription from RNA polymerase II promoter in response to acidic pH lipid metabolic process

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PCK1 Polyclonal Antibody

PCK1 Antibody (16754-1-AP) in ICC/IF

Product Details

16754-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

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