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PCOLCE Antibody (PA5-110084) in WB
Western blot analysis of PCOLCE in extracts of U-251MG cells. Samples were incubated with PCOLCE Polyclonal antibody (Product # PA5-110084) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Product Details
PA5-110084
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 170-449 of human PCOLCE (NP_002584.2).
Conjugate
Form
Liquid
Concentration
1.86 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2855495
Product Specific Information
Immunogen sequence: LTTPNWPESD YPPGISCSWH IIAPPDQVIA LTFEKFDLEP DTYCRYDSVS VFNGAVSDDS RRLGKFCGDA VPGSISSEGN ELLVQFVSDL SVTADGFSAS YKTLPRGTAK EGQGPGPKRG TEPKVKLPPK SQPPEKTEES PSAPDAPTCP KQCRRTGTLQ SNFCASSLVV TATVKSMVRE PGEGLAVTVS LIGAYKTGGL DLPSPPTGAS LKFYVPCKQC PPMKKGVSYL LMGQVEENRG PVLPPESFVV LHRPNQDQIL TNLSKRKCPS QPVRAAASQD
Target Information
Fibrillar collagen types I-III are synthesized as precursor molecules known as procollagens. These precursors contain amino- and carboxyl-terminal peptide extensions known as N- and C-propeptides, respectively, which are cleaved, upon secretion of procollagen from the cell, to yield the mature triple helical, highly structured fibrils. This gene encodes a glycoprotein which binds and drives the enzymatic cleavage of type I procollagen and heightens C-proteinase activity.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: PCPE-1; Procollagen C-endopeptidase enhancer 1; procollagen C-proteinase enhancer 1; Procollagen COOH-terminal proteinase enhancer 1; procollagen, type 1, COOH-terminal proteinase enhancer; Type 1 procollagen C-proteinase enhancer protein; Type I procollagen COOH-terminal proteinase enhancer
Gene Aliases: PCOLCE; PCPE; PCPE-1; PCPE1
UniProt ID: (Human) Q15113
Entrez Gene ID: (Human) 5118
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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