Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 1
PCOLCE Antibody (MA5-55514) in WB
Western Blot analysis of various lysates using PCOLCE Recombinant Monoclonal Antibody (Product # MA5-55514) at 1:1,000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Negative control (NC): THP-1 Exposure time: 30s.
Product Details
MA5-55514
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
4B8J4
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 304-449 of human TLR4.
Conjugate
Form
Liquid
Concentration
1.43 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.05% ProClin 300
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Dry ice (international)
Product Specific Information
Immunogen Sequence: EKTEESPSAP DAPTCPKQCR RTGTLQSNFC ASSLVVTATV KSMVREPGEG LAVTVSLIGA YKTGGLDLPS PPTGASLKFY VPCKQCPPMK KGVSYLLMGQ VEENRGPVLP PESFVVLHRP NQDQILTNLS KRKCPSQPVR AAASQD
Target Information
Fibrillar collagen types I-III are synthesized as precursor molecules known as procollagens. These precursors contain amino- and carboxyl-terminal peptide extensions known as N- and C-propeptides, respectively, which are cleaved, upon secretion of procollagen from the cell, to yield the mature triple helical, highly structured fibrils. This gene encodes a glycoprotein which binds and drives the enzymatic cleavage of type I procollagen and heightens C-proteinase activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: astroglial cell transcript 2; P14; PCPE-1; Procollagen C-endopeptidase enhancer 1; procollagen C-endopeptidase enhancer protein; procollagen C-proteinase enhancer 1; procollagen C-proteinase enhancer protein; Procollagen COOH-terminal proteinase enhancer 1; procollagen, type 1, COOH-terminal proteinase enhancer; Type 1 procollagen C-proteinase enhancer protein; Type I procollagen COOH-terminal proteinase enhancer
Gene Aliases: Astt-2; Astt2; P14; PCOLCE; PCPE; PCPE-1; PCPE1
UniProt ID: (Human) Q15113, (Mouse) Q61398, (Rat) O08628
Entrez Gene ID: (Human) 5118, (Mouse) 18542, (Rat) 29569
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support