Invitrogen

PD-1 Monoclonal Antibody (7A11B1)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

5 Published Figures

4 References

View all (275) PD-1 (CD279) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

PD-1 Antibody in Immunohistochemistry (IHC)

FIGURE: 1 / 11

PD-1 Antibody (MA5-15780) in IHC

Immunofluorescence of PD-1 in human spleen tissue with PD-1 Monoclonal Antibody (7A11B1) (Product # MA5-15780) at 20 µg/mL. Green: PD-1 Antibody [7A11B1] (PM-5177) Blue: DAPI staining

PD-1 Antibody in Western Blot, Immunoprecipitation (WB, IP)

Product Image Gallery

PD-1 Antibody — Fig. 3 Effects of PD-1/PD-L1 overexpression and knockdown on brain cell death and neuronal degeneration after ICH. a ICH rats accepted intracerebroventricular injection of pDNA or siRNAs as indicated. b Western blot analysis of the efficiency of PD-1 and PD-L1 overexpression or knockdown in brain of ICH rats. Quantification of relative protein levels of PD-1 and PD-L1 was shown in c. c Data are expressed as mean +- SEM, n = 6. Triple asterisks indicate p < 0.001 vs. ICH + vector group; triple pound signs indicate p < 0.001 vs. ICH + control siRNA group. d Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining. Sections were labeled by TUNEL ( green ) to detect apoptotic brain cells and counterstained with DAPI ( blue ) to detect nuclei. Arrows point to TUNEL-positive cells. Scale bar = 64 mum. e Percentage of TUNEL-positive cells. Data are expressed as mean +- SEM, n = 6. Triple asterisks indicate p < 0.001 vs. sham group; double pound signs indicate p < 0.01 vs. ICH + vector group; ampersand indicates p < 0.05, double ampersands indicate p < 0.01 vs. ICH + control siRNA group. f Fluoro-jade B (FJB) staining. Arrows point to FJB-positive cells. Scale bar = 64 mum. The number of FJB-positive brain cells was calculated. g Data are expressed as mean +- SEM, n = 6. Triple asterisks indicate p < 0.001 vs. sham group; double pound signs indicate p < 0.01 vs. ICH + vector group; double ampersands indicate p < 0.01, triple ampersands indicate p < 0.001 vs. ICH {KD}

Product Details

MA5-15780

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 1 publication 1 publication

Immunohistochemistry (IHC)

2.5 µg/mL

View 2 publications 2 publications

Immunoprecipitation (IP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

7A11B1

Immunogen

A ~150 amino acid recombinant protein from near the amino terminus of mouse PD-1

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11152225

Product Specific Information

A suggested positive control is PD-1 recombinant protein.

Target Information

Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases. Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Knockdown of Leptin Receptor Affects Macrophage Phenotype in the Tumor Microenvironment Inhibiting Breast Cancer Growth and Progression.

Cancers

Gelsomino L,Naimo GD,Malivindi R,Augimeri G,Panza S,Giordano C,Barone I,Bonofiglio D,Mauro L,Catalano S,Andò S

MA5-15780 was used in Immunohistochemistry to evaluate how the tumour cell-macrophage interaction and breast cancer phenotype are affected by leptin receptor knockdown.

Mon Jul 27 00:00:00 EDT 2020

Knockdown of Leptin Receptor Affects Macrophage Phenotype in the Tumor Microenvironment Inhibiting Breast Cancer Growth and Progression.

Cancers

Gelsomino L,Naimo GD,Malivindi R,Augimeri G,Panza S,Giordano C,Barone I,Bonofiglio D,Mauro L,Catalano S,Andò S

MA5-15780 was used in Immunohistochemistry to evaluate how the tumour cell-macrophage interaction and breast cancer phenotype are affected by leptin receptor knockdown.

Mon Jul 27 00:00:00 EDT 2020

Roles of programmed death protein 1/programmed death-ligand 1 in secondary brain injury after intracerebral hemorrhage in rats: selective modulation of microglia polarization to anti-inflammatory phenotype.

Journal of neuroinflammation

Wu J,Sun L,Li H,Shen H,Zhai W,Yu Z,Chen G

MA515780 was used in immunocytochemistry, immunohistochemistry - paraffin section, immunoprecipitation, and western blot to assess the critical role of programmed death protein 1/programmed death-ligand 1 in the polarization of microglia during intracerebral hemorrhage-induced secondary brain injury

Tue Feb 14 00:00:00 EST 2017

Journal of neuroinflammation

Wu J,Sun L,Li H,Shen H,Zhai W,Yu Z,Chen G

Tue Feb 14 00:00:00 EST 2017

Journal of neuroinflammation

Wu J,Sun L,Li H,Shen H,Zhai W,Yu Z,Chen G

Tue Feb 14 00:00:00 EST 2017

Bioinformatics

Protein Aliases: CD279; hPD1; mPD-1; programmed cell death 1 protein; Programmed cell death protein 1; programmed cell death protein 1-like; Protein PD-1; Protein PD1; sCD279; soluble CD279; systemic lupus erythematosus susceptibility 2

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Gene Aliases: CD279; hPD-1; hPD-l; hSLE1; Ly101; PD-1; PD1; Pdc1; PDCD1; SLEB2

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UniProt ID: (Human) Q15116, (Mouse) Q02242

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Entrez Gene ID: (Human) 5133, (Rat) 301626, (Mouse) 18566

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Function(s)

signal transducer activity protein binding molecular_function

Process(es)

negative regulation of tolerance induction apoptotic process humoral immune response signal transduction multicellular organism development T cell costimulation negative regulation of apoptotic process positive regulation of T cell apoptotic process biological_process positive regulation of apoptotic process immune system process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PD-1 Monoclonal Antibody (7A11B1)

PD-1 Antibody (MA5-15780) in IHC

Product Details

MA5-15780

Western Blot (WB)

Immunohistochemistry (IHC)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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