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Invitrogen
PD-1 (CD279) Chimeric Recombinant Rabbit Monoclonal Antibody (J43)
FIGURE: 1 / 4
PD-1 (CD279) Chimeric Antibody (MA5-48236) in ELISA
ELISA analysis of PD-1 (CD279) chimeric monoclonal antibody (Product # MA5-48236) was performed by coating plate with PD-1 (CD279)-Fc-fusion protein at a concentration of 5 µg/mL. A 3-fold serial dilution from 10,000 ng/mL of the antibody was done. For detection, a 1:4,000 dilution of HRP-labelled anti-rabbit antibody was used.
Product Details
MA5-48236
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG, lambda
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
J43
Immunogen
This antibody was raised by immunising Armenian hamsters with B12 cells, a PD-1 cDNA transfectant of BHK cells.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.02% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_3074678
Product Specific Information
Specificity: This antibody is specific for murine PD-1.
Target Information
Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases. Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD279; hPD1; mPD-1; Programmed cell death protein 1; Protein PD-1; Protein PD1; sCD279; soluble CD279
Gene Aliases: Ly101; PD-1; Pd1; Pdc1; Pdcd1
UniProt ID: (Mouse) Q02242
Entrez Gene ID: (Mouse) 18566
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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