Invitrogen

PD-L1 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

13 Published Figures

17 References

View all (230) PD-L1 (CD274) antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

PD-L1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 24

PD-L1 Antibody (PA5-28115) in ICC/IF

Immunocytochemistry-Immunofluorescence analysis of PD-L1 was performed in MDA-MB-231 (left) and HeLa (right) cells fixed in ice-cold MeOH for 5 min. Green: PD-L1 Polyclonal Antibody (Product # PA5-28115) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

PD-L1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PD-L1 Antibody in Immunohistochemistry (IHC)

PD-L1 Antibody in Western Blot, Flow Cytometry (WB, Flow)

Product Image Gallery

PD-L1 Antibody — Antibody specificity was demonstrated by shRNA mediated knockdown of the target protein. A431 cells were transfected with PD-L1 shRNA and decrease in signal intensity was observed in western blot application using PD-L1 antibody (Product # PA5-28115). {KD}

Product Details

PA5-28115

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 9 publications 9 publications

Immunohistochemistry (IHC)

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

Immunocytochemistry (ICC/IF)

1:100-1:1,000

Flow Cytometry (Flow)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to a region within amino acids 230 and 290 of CD274

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.97 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2545591

Product Specific Information

Recommended positive controls: A431, MDA-MB-231, MDA-MB-231 (24 µg/mL Tunicamycin treatment for 16 hr), MDA-MB-231, A549 (100 ng/mL IFN-gamma treatment for 48 hr), A431 (100 ng/mL IFN-gamma treatment for 48 hr).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Programmed death receptor ligand 1 (PD-L1, also called B7-H1) is a recently described B7 family member. To date, one specific receptor has been identified that can be ligated by PD-L1. This receptor, programmed death receptor 1 (PD-1), has been shown to negatively regulate T-cell receptor (TCR) signaling. Upon ligating its receptor, PD-L1 has been reported to decrease TCR-mediated proliferation and cytokine production. PD-L1 expression was found to be abundant on many murine and human cancers and could be further up-regulated upon IFN-gamma stimulation. Thus, PD-L1 might play an important role in tumor immune evasion.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Iron-mediated oxidative stress induces PD-L1 expression via activation of c-Myc in lung adenocarcinoma.

Frontiers in cell and developmental biology

Battaglia AM,Sacco A,Aversa I,Santamaria G,Palmieri C,Botta C,De Stefano R,Bitetto M,Petriaggi L,Giorgio E,Faniello CM,Costanzo F,Biamonte F

PA5-28115 was used in Western Blotting to demonstrate that iron abundance within the TME may enhance PD-L1 expression in LUAD and, thus, open the way for the identification of possible combinatorial strategies that take into account the iron levels within the TME to improve the outcomes of LUAD patients treated with anti-PD-1/PD-L1-based therapies.

Sat Jul 01 00:00:00 EDT 2023

Iron-mediated oxidative stress induces PD-L1 expression via activation of c-Myc in lung adenocarcinoma.

Frontiers in cell and developmental biology

Battaglia AM,Sacco A,Aversa I,Santamaria G,Palmieri C,Botta C,De Stefano R,Bitetto M,Petriaggi L,Giorgio E,Faniello CM,Costanzo F,Biamonte F

Sat Jul 01 00:00:00 EDT 2023

Immunohistochemical Profiling of PD-1, PD-L1, CD8, MSI, and p53 and Prognostic Implications in Advanced Serous Ovarian Carcinoma: A Retrospective Study.

Journal of personalized medicine

Dergham AP,Vaz de Paula CB,Nagashima S,Olandoski M,de Noronha L,Sotomaior VS

PA5-28115 was used in Immunohistochemistry to evaluate the expression of PD-1, PD-L1, CD8, MSI (MLH1, MSH2, MSH6, and PMS2), and p53 in the paraffin samples of high-grade serous ovarian carcinoma.

Mon Jun 26 00:00:00 EDT 2023

Therapeutic and immunomodulatory potential of pazopanib in malignant phyllodes tumor.

NPJ breast cancer

Ng DYX,Li Z,Lee E,Kok JST,Lee JY,Koh J,Ng CC,Lim AH,Liu W,Ng SR,Lim KS,Huang XX,Hong JH,Guan P,Sim Y,Thike AA,Nasir NDM,Li S,Tan PH,Teh BT,Chan JY

Published figure using PD-L1 polyclonal antibody (Product # PA5-28115) in Western Blot

Fri Apr 01 00:00:00 EDT 2022

Evaluation PD-L1, CD8 and CD20 as early predictor and tracking markers for breast cancer (BC) in Egypt.

Heliyon

Hamed MM,Gouida MS,Abd El-Aziz SR,El-Sokkary AMA

PA5-28115 was used in Immunohistochemistry to conclude that PD-L1, CD8, and CD20 are considered as early predictor and tracking markers for breast cancer.

Sun May 01 00:00:00 EDT 2022

Licorice extract inhibits growth of non-small cell lung cancer by down-regulating CDK4-Cyclin D1 complex and increasing CD8⁺ T cell infiltration.

Cancer cell international

Zhu J,Huang R,Yang R,Xiao Y,Yan J,Zheng C,Xiao W,Huang C,Wang Y

Published figure using PD-L1 polyclonal antibody (Product # PA5-28115) in Western Blot

Tue Oct 12 00:00:00 EDT 2021

Deep Phenotypic Characterisation of CTCs by Combination of Microfluidic Isolation (IsoFlux) and Imaging Flow Cytometry (ImageStream).

Cancers

Ruiz-Rodríguez AJ,Molina-Vallejo MP,Aznar-Peralta I,González Puga C,Cañas García I,González E,Lorente JA,Serrano MJ,Garrido-Navas MC

PA5-28115 was used in Flow cytometry/Cell sorting to develop a novel protocol combining two platforms (IsoFluxTM and ImageStream®X) to improve circulating tumour cells evaluation.

Mon Dec 20 00:00:00 EST 2021

MLLT6 maintains PD-L1 expression and mediates tumor immune resistance.

EMBO reports

Sreevalsan S,Döring M,Paszkowski-Rogacz M,Brux M,Blanck C,Meyer M,Momburg F,Buchholz F,Theis M

PA5-28115 was used in Western Blotting to establish MLLT6 as a regulator of oncogenic and interferon-γ-associated immune resistance.

Thu Dec 03 00:00:00 EST 2020

The deubiquitinase USP22 regulates PD-L1 degradation in human cancer cells.

Cell communication and signaling : CCS

Wang Y,Sun Q,Mu N,Sun X,Wang Y,Fan S,Su L,Liu X

PA5-28115 was used in Western Blotting to investigate the function of USP22 upon PD-L1 in human cancer cells.

Tue Jul 14 00:00:00 EDT 2020

The deubiquitinase USP22 regulates PD-L1 degradation in human cancer cells.

Cell communication and signaling : CCS

Wang Y,Sun Q,Mu N,Sun X,Wang Y,Fan S,Su L,Liu X

PA5-28115 was used in Western Blotting to investigate the function of USP22 upon PD-L1 in human cancer cells.

Tue Jul 14 00:00:00 EDT 2020

Rapidly fatal pneumonitis from immunotherapy and concurrent SARS-CoV-2 infection in a patient with newly diagnosed lung cancer.

medRxiv : the preprint server for health sciences

Lovly CM,Boyd KL,Gonzalez-Ericsson PI,Lowe CL,Brown HM,Hoffman RD,Sterling BC,Kapp ME,Johnson DB,Kopparapu PR,Iams WT,Warren MA,Noto MJ,Rini BI,Jagasia M,Das SR,Balko JM

PA5-28115 was used in Immunohistochemistry to report a case of acute lung injury in a lung cancer patient initially treated for ICI-pneumonitis and later found to have concurrent SARS-CoV-2 infection.

Fri May 01 00:00:00 EDT 2020

Rapidly fatal pneumonitis from immunotherapy and concurrent SARS-CoV-2 infection in a patient with newly diagnosed lung cancer.

medRxiv : the preprint server for health sciences

Lovly CM,Boyd KL,Gonzalez-Ericsson PI,Lowe CL,Brown HM,Hoffman RD,Sterling BC,Kapp ME,Johnson DB,Kopparapu PR,Iams WT,Warren MA,Noto MJ,Rini BI,Jagasia M,Das SR,Balko JM

Fri May 01 00:00:00 EDT 2020

A case report of clonal EBV-like memory CD4⁺ T cell activation in fatal checkpoint inhibitor-induced encephalitis.

Nature medicine

Johnson DB,McDonnell WJ,Gonzalez-Ericsson PI,Al-Rohil RN,Mobley BC,Salem JE,Wang DY,Sanchez V,Wang Y,Chastain CA,Barker K,Liang Y,Warren S,Beechem JM,Menzies AM,Tio M,Long GV,Cohen JV,Guidon AC,O'Hare M,Chandra S,Chowdhary A,Lebrun-Vignes B,Goldinger SM,Rushing EJ,Buchbinder EI,Mallal SA,Shi C,Xu Y,Moslehi JJ,Sanders ME,Sosman JA,Balko JM

PA5-28115 was used in Immunohistochemistry to identify if CD4+ and CD8+ T cells are potential culprits of checkpoint inhibitor-associated immune encephalitis.

Thu Aug 01 00:00:00 EDT 2019

HDAC6‑selective inhibitor synergistically enhances the anticancer activity of immunomodulatory drugs in multiple myeloma.

International journal of oncology

Won HR,Lee DH,Yeon SK,Ryu HW,Kim GW,Kwon SH

PA5-28115 was used in Western Blotting to investigate the interaction between the HDAC6 inhibitor, A452, and immunomodulatory drugs on dexamethasone-sensitive and -resistant multiple myeloma cells compared with the current clinically tested HDAC6 inhibitor, ACY-1215.

Thu Aug 01 00:00:00 EDT 2019

Immuno-PET Imaging of the Programmed Cell Death-1 Ligand (PD-L1) Using a Zirconium-89 Labeled Therapeutic Antibody, Avelumab.

Molecular imaging

Jagoda EM,Vasalatiy O,Basuli F,Opina ACL,Williams MR,Wong K,Lane KC,Adler S,Ton AT,Szajek LP,Xu B,Butcher D,Edmondson EF,Swenson RE,Greiner J,Gulley J,Eary J,Choyke PL

Published figure using PD-L1 polyclonal antibody (Product # PA5-28115) in Immunohistochemistry

Tue Aug 27 00:00:00 EDT 2019

The HDAC6 inhibitor ACY‑1215 enhances the anticancer activity of oxaliplatin in colorectal cancer cells.

International journal of oncology

Lee DH,Won HR,Ryu HW,Han JM,Kwon SH

PA5-28115 was used in Western Blotting to suggest that a therapeutic strategy that combines ACY-1215 and oxaliplatin warrants attention for the treatment of solid tumors, including CRC.

Wed Aug 01 00:00:00 EDT 2018

Cancer immunotherapy using PolyPurine Reverse Hoogsteen hairpins targeting the PD-1/PD-L1 pathway in human tumor cells.

PloS one

Medina Enríquez MM,Félix AJ,Ciudad CJ,Noé V

Published figure using PD-L1 polyclonal antibody (Product # PA5-28115) in Western Blot

Wed Apr 17 00:00:00 EDT 2019

A Unique Cellular and Molecular Microenvironment Is Present in Tertiary Lymphoid Organs of Patients with Spontaneous Prostate Cancer Regression.

Frontiers in immunology

García-Hernández ML,Uribe-Uribe NO,Espinosa-González R,Kast WM,Khader SA,Rangel-Moreno J

Published figure using PD-L1 polyclonal antibody (Product # PA5-28115) in Immunofluorescence

Wed Nov 20 00:00:00 EST 2019

Essential role of HDAC6 in the regulation of PD-L1 in melanoma.

Molecular oncology

M L,P PV,T K,M P,E S,J P,K V W,C L,F C,S D,M SKS,M M,A K,J PI,A S,E S,J W,E M S,A V

PA5-28115 was used in western blot to characterize regulation of PD-L1 in melanoma by HDAC6

Sun May 01 00:00:00 EDT 2016

Essential role of HDAC6 in the regulation of PD-L1 in melanoma.

Molecular oncology

M L,P PV,T K,M P,E S,J P,K V W,C L,F C,S D,M SKS,M M,A K,J PI,A S,E S,J W,E M S,A V

PA5-28115 was used in western blot to characterize regulation of PD-L1 in melanoma by HDAC6

Sun May 01 00:00:00 EDT 2016

Bioinformatics

Protein Aliases: B7 homolog 1; B7-H1; CD274; CD274 antigen; PD-L1; PDCD1 ligand 1; pdl-1; Programmed cell death 1 ligand 1; sCD274; soluble CD274; soluble PD L1; sPD L1

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Gene Aliases: B7-H; B7H1; CD274; PD-L1; PDCD1L1; PDCD1LG1; PDL1

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UniProt ID: (Human) Q9NZQ7

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Entrez Gene ID: (Human) 29126

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Function(s)

protein binding immunoglobulin receptor superfamily

Process(es)

immune response signal transduction cell surface receptor signaling pathway positive regulation of cell migration T cell costimulation negative regulation of interferon-gamma production negative regulation of interleukin-10 production response to cytokine positive regulation of T cell proliferation regulation of activated T cell proliferation negative regulation of activated T cell proliferation regulation of T cell apoptotic process toxin transport negative regulation of tumor necrosis factor superfamily cytokine production positive regulation of interleukin-10 secretion

Performance Guarantee

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PD-L1 Polyclonal Antibody

PD-L1 Antibody (PA5-28115) in ICC/IF

Product Details

PA5-28115

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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