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Invitrogen
PDCD4 Recombinant Rabbit Monoclonal Antibody (1H15L6)
Advanced Verification
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 6
PDCD4 Antibody (702494) in ICC/IF
For immunofluorescence analysis, serum starved MCF-7 cells were fixed and permeabilized for detection of endogenous PDCD4 using Anti-PDCD4 Recombinant Rabbit Monoclonal Antibody (Product # 702494, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of PDCD4 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (... View More
Product Details
702494
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
1H15L6
Immunogen
Protein corresponding to human PDCD4 (aa157-aa320)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2688159
Product Specific Information
This antibody is predicted to react with Monkey, Cat, Horse, Sheep
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
Apoptosis, also known as programmed cell death, plays major roles in development and normal tissue turnover in addition to tumor formation. During this process, the expression patterns of numerous genes are radically altered. One such gene is the programmed cell death protein 4 (PDCD4), whose expression was found to be upregulated in all cell lines following the onset of apoptosis. PDCD4 encodes a tumor suppressor protein whose expression is lost in carcinomas of breast, colon, lung and prostate. It can bind to and inhibit the helicase activity of the eukaryotic translation initiation factor 4A and inhibit the transactivation and transformation mediated by the transcription factor AP-1. The kinase Akt regulates PDCD4 by phosphorylation, decreasing the ability of PDCD4 to interfere with the transactivation of AP-1-responsive promoter by c-Jun. There are two known isoforms of PDCD4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: MGC33046; MGC33047; Neoplastic transformation inhibitor protein; nuclear antigen H731; Nuclear antigen H731-like; Programmed cell death protein 4; Protein 197/15a; Protein MA-3; RP11-348N5.4; Topoisomerase-inhibitor suppressed protein
Gene Aliases: D19Ucla1; H731; Ma3; PDCD4; Tis
UniProt ID: (Human) Q53EL6, (Mouse) Q61823
Entrez Gene ID: (Human) 27250, (Mouse) 18569
apoptotic process
cell aging
positive regulation of smooth muscle cell apoptotic process
negative regulation of apoptotic process
negative regulation of JUN kinase activity
negative regulation of cell cycle
negative regulation of transcription, DNA-templated
negative regulation of myofibroblast differentiation
regulation of protein metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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