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PDCD6IP Antibody (MA5-36229) in ICC/IF
Immunocytochemistry analysis of PDCD6IP in U20S cells. Antigen retrieval was performed with enzyme antigen retrieval (15 min). Samples were blocked with 10% goat serum and incubated in PDCD6IP monoclonal antibody (Product # MA5-36229) at a dilution of 2 µg/mL (overnight, 4°C), followed by Dylight 488 conjugated goat anti-mouse IgG (30 min, 37°C) and DAPI at a dilution of 5-10 µg/1x10^6 cells (30 min, 20°C).
Product Details
MA5-36229
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
14D10
Immunogen
E.coli-derived human PDCD6IP recombinant protein (Position: A2-D330). Human PDCD6IP shares 96.7% and 95.2% amino acid (aa) sequence identity with mouse and rat PDCD6IP, respectively.
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2884063
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Target Information
Apoptotic linked-gene-product 2 (ALG-2) interacting protein X (ALIX) is a conserved adaptor protein which is ubiquitously expressed. Alix was originally reported to play a role in apoptosis but has recently been shown to be involved in other cellular mechanisms including endosomal sorting, endocytosis, viral budding and actin cytoskeleton assembly.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ALG-2 interacting protein 1; ALG-2 interacting protein X; ALG-2-interacting protein 1; ALG-2-interacting protein X; Alg2-interacting protein 1; Alg2-interacting protein X; apoptosis-linked gene 2-interacting protein X; dopamine receptor interacting protein 4; E2F1-inducible protein; Eig2; Hp95; MGC17003; PDCD6-interacting protein; Programmed cell death 6-interacting protein
Gene Aliases: AI480591; AIP1; ALIX; AW544830; C76364; DRIP4; Eig2; HP95; KIAA1375; mKIAA1375; PDCD6IP; RGD1561176
UniProt ID: (Human) Q8WUM4, (Rat) Q9QZA2, (Mouse) Q9WU78
Entrez Gene ID: (Human) 10015, (Rat) 501083, (Mouse) 18571
cell separation after cytokinesis
apoptotic process
nucleus organization
mitotic metaphase plate congression
regulation of centrosome duplication
protein transport
viral life cycle
multivesicular body assembly
viral budding via host ESCRT complex
ubiquitin-independent protein catabolic process via the multivesicular body sorting pathway
regulation of mitotic spindle assembly
positive regulation of exosomal secretion
regulation of extracellular exosome assembly
positive regulation of extracellular exosome assembly
cell division
transport
cell cycle
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