Proteintech

PDE4D Polyclonal Antibody

PDE4D Antibody in Immunocytochemistry (ICC/IF)

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PDE4D Antibody (12918-1-AP) in ICC/IF

Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using (Product # 12918-1-AP) (PDE4D antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

PDE4D Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

Product Details

12918-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 18 publications 18 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:100

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

PDE4D Fusion Protein Ag3588 (170-518 aa encoded by BC036319)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.14 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: YHNNIHAAD VVQSTHVLLS TPALEAVFTD LEILAAIFAS AIHDVDHPGV FNQFLINTNS ELALMYNDSS VLENHHLAVG FKLLQEENCD IFQNLTKKQR QSLRKMVIDI VLATDMSKHM NLLADLKTMV ETKKVTSSGV LLLDNYSDRI QVLQNMVHCA DLSNPTKPLQ LYRQWTDRIM EEFFRQGDRE RERGMEISPM CDKHNASVEK SQVGFIDYIV HPLWETWADL VHPDAQDILD TLEDNREWYQ STIPQSPSPA PDDPEEGRQG QTEKFQFELT LEEDGESDTE KDSGSQVEED TSCSDSKTLC TQDSESTEIP LDEQVEEEAV GEEEESQPEA CVIDDRSPDT (170-518 aa encoded by BC036319)

Target Information

This gene encodes one of four mammalian counterparts to the fruit fly 'dunce' gene. The encoded protein has 3',5'-cyclic-AMP phosphodiesterase activity and degrades cAMP, which acts as a signal transduction molecule in multiple cell types. This gene uses different promoters to generate multiple alternatively spliced transcript variants that encode functional proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

An Artemisia scoparia extract attenuates glucocorticoid-induced lipolysis in adipocytes.

Obesity (Silver Spring, Md.)

Harvey I,Stephens JM

Sat Jul 01 00:00:00 EDT 2023

PDE4D/cAMP/IL-23 axis determines the immunotherapy efficacy of lung adenocarcinoma via activating the IL-9 autocrine loop of cytotoxic T lymphocytes.

Cancer letters

Feng B,Pan B,Huang J,Du Y,Wang X,Wu J,Ma R,Shen B,Huang G,Feng J

Sat Jul 01 00:00:00 EDT 2023

PDE4D/cAMP/IL-23 axis determines the immunotherapy efficacy of lung adenocarcinoma via activating the IL-9 autocrine loop of cytotoxic T lymphocytes.

Cancer letters

Feng B,Pan B,Huang J,Du Y,Wang X,Wu J,Ma R,Shen B,Huang G,Feng J

Sat Jul 01 00:00:00 EDT 2023

Synergistic effect of constituent drugs of Baibutang on improving Yin-deficiency pulmonary fibrosis in rats.

Journal of ethnopharmacology

Fu S,Song X,Tang X,Qian X,Du Z,Hu Y,Xu X,Zhang M

Mon Apr 24 00:00:00 EDT 2023

Overexpression of PDE4D in mouse liver is sufficient to trigger NAFLD and hypertension in a CD36-TGF-β1 pathway: therapeutic role of roflumilast.

Pharmacological research

Tao X,He H,Peng J,Xu R,Fu J,Hu Y,Li L,Yang X,Feng X,Zhang C,Zhang L,Yu X,Shen A,Huang K,Fu Q

Sat Jan 01 00:00:00 EST 2022

FGF1 and insulin control lipolysis by convergent pathways.

Cell metabolism

Sancar G,Liu S,Gasser E,Alvarez JG,Moutos C,Kim K,van Zutphen T,Wang Y,Huddy TF,Ross B,Dai Y,Zepeda D,Collins B,Tilley E,Kolar MJ,Yu RT,Atkins AR,van Dijk TH,Saghatelian A,Jonker JW,Downes M,Evans RM

Tue Jan 04 00:00:00 EST 2022

Targeting PDE4 as a promising therapeutic strategy in chronic ulcerative colitis through modulating mucosal homeostasis.

Acta pharmaceutica Sinica. B

Li H,Zhang Y,Liu M,Fan C,Feng C,Lu Q,Xiang C,Lu H,Yang X,Wu B,Zou D,Tang W

Sat Jan 01 00:00:00 EST 2022

PDE4D binds and interacts with YAP to cooperatively promote HCC progression.

Cancer letters

Ren H,Chen Y,Ao Z,Cheng Q,Yang X,Tao H,Zhao L,Shen A,Li P,Fu Q

Wed Aug 10 00:00:00 EDT 2022

PDE4D binds and interacts with YAP to cooperatively promote HCC progression.

Cancer letters

Ren H,Chen Y,Ao Z,Cheng Q,Yang X,Tao H,Zhao L,Shen A,Li P,Fu Q

Wed Aug 10 00:00:00 EDT 2022

PDE4D binds and interacts with YAP to cooperatively promote HCC progression.

Cancer letters

Ren H,Chen Y,Ao Z,Cheng Q,Yang X,Tao H,Zhao L,Shen A,Li P,Fu Q

Wed Aug 10 00:00:00 EDT 2022

MicroRNA-137-3p Improves Nonalcoholic Fatty Liver Disease through Activating AMPKα.

Analytical cellular pathology (Amsterdam)

Yu Y,He C,Tan S,Huang M,Guo Y,Li M,Zhang Q

Mon Mar 28 00:00:00 EDT 2022

Evidence for reprogramming of monocytes into reparative alveolar macrophages in vivo by targeting PDE4b.

American journal of physiology. Lung cellular and molecular physiology

Rochford I,Joshi JC,Rayees S,Anwar M,Akhter MZ,Yalagala L,Banerjee S,Mehta D

Fri Oct 01 00:00:00 EDT 2021

Inhibition of PDE4/PDE4B improves renal function and ameliorates inflammation in cisplatin-induced acute kidney injury.

American journal of physiology. Renal physiology

Xu M,Yu X,Meng X,Huang S,Zhang Y,Zhang A,Jia Z

Sun Mar 01 00:00:00 EST 2020

Hyperinsulinemia promotes heterologous desensitization of β₂ adrenergic receptor in airway smooth muscle in obesity.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Xu R,Gopireddy RR,Wu Y,Wu L,Tao X,Shao J,Wang W,Li L,Jovanovic A,Xu B,Kenyon NJ,Lu Q,Xiang YK,Fu Q

Sun Mar 01 00:00:00 EST 2020

Upregulation of miR-219a-5p Decreases Cerebral Ischemia/Reperfusion Injury In Vitro by Targeting Pde4d.

Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association

Lu MY,Wu JR,Liang RB,Wang YP,Zhu YC,Ma ZT,Zhang H,Zan J,Tan W

Mon Jun 01 00:00:00 EDT 2020

DC591017, a phosphodiesterase-4 (PDE4) inhibitor with robust anti-inflammation through regulating PKA-CREB signaling.

Biochemical pharmacology

Li H,Li J,Zhang X,Feng C,Fan C,Yang X,Zhang R,Zhu F,Zhou Y,Xu Y,Liu H,Tang W

Wed Jul 01 00:00:00 EDT 2020

DC591017, a phosphodiesterase-4 (PDE4) inhibitor with robust anti-inflammation through regulating PKA-CREB signaling.

Biochemical pharmacology

Li H,Li J,Zhang X,Feng C,Fan C,Yang X,Zhang R,Zhu F,Zhou Y,Xu Y,Liu H,Tang W

Wed Jul 01 00:00:00 EDT 2020

Inhibition of phosphodiesterase-4 attenuates murine ulcerative colitis through interference with mucosal immunity.

British journal of pharmacology

Li H,Fan C,Feng C,Wu Y,Lu H,He P,Yang X,Zhu F,Qi Q,Gao Y,Zuo J,Tang W

Mon Jul 01 00:00:00 EDT 2019

High expression of PDE4D correlates with poor prognosis and clinical progression in pancreaticductal adenocarcinoma.

Journal of Cancer

Liu F,Ma J,Wang K,Li Z,Jiang Q,Chen H,Li W,Xia J

Thu Oct 19 00:00:00 EDT 2023

Molecular inotropy mediated by cardiac miR-based PDE4D/PRKAR1α/phosphoprotein signaling.

Scientific reports

Bedada FB,Martindale JJ,Arden E,Metzger JM

Fri Nov 11 00:00:00 EST 2016

Molecular inotropy mediated by cardiac miR-based PDE4D/PRKAR1α/phosphoprotein signaling.

Scientific reports

Bedada FB,Martindale JJ,Arden E,Metzger JM

Fri Nov 11 00:00:00 EST 2016

Salt-Inducible Kinase 1 Terminates cAMP Signaling by an Evolutionarily Conserved Negative-Feedback Loop in β-Cells.

Diabetes

Kim MJ,Park SK,Lee JH,Jung CY,Sung DJ,Park JH,Yoon YS,Park J,Park KG,Song DK,Cho H,Kim ST,Koo SH

Tue Sep 01 00:00:00 EDT 2015

Interplay between the E2F pathway and β-adrenergic signaling in the pathological hypertrophic response of myocardium.

Journal of molecular and cellular cardiology

Major JL,Salih M,Tuana BS

Wed Jul 01 00:00:00 EDT 2015

Interplay between the E2F pathway and β-adrenergic signaling in the pathological hypertrophic response of myocardium.

Journal of molecular and cellular cardiology

Major JL,Salih M,Tuana BS

Wed Jul 01 00:00:00 EDT 2015

miR-199a Links MeCP2 with mTOR Signaling and Its Dysregulation Leads to Rett Syndrome Phenotypes.

Cell reports

Tsujimura K,Irie K,Nakashima H,Egashira Y,Fukao Y,Fujiwara M,Itoh M,Uesaka M,Imamura T,Nakahata Y,Yamashita Y,Abe T,Takamori S,Nakashima K

Tue Sep 22 00:00:00 EDT 2015

miR-199a Links MeCP2 with mTOR Signaling and Its Dysregulation Leads to Rett Syndrome Phenotypes.

Cell reports

Tsujimura K,Irie K,Nakashima H,Egashira Y,Fukao Y,Fujiwara M,Itoh M,Uesaka M,Imamura T,Nakahata Y,Yamashita Y,Abe T,Takamori S,Nakashima K

Tue Sep 22 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: 3',5'-cyclic-AMP phosphodiesterase 4D; cAMP-specific 3',5'-cyclic phosphodiesterase 4D; cAMP-specific phosphodiesterase 4D; cAMP-specific phosphodiesterase PDE4D; cAMP-specific phosphodiesterase PDE4D6; cAMP-specific phosphodiesterase splice variant PDE4D8; cyclic AMP specific phosphodiesterase PDE4D5A; DKFZp686M11213; DPDE3; dunce; FLJ97311; HGNC:8783; HS PDE4DN2; PDE43; PDE4D3; PDE4D7; Phosphodiesterase 4D cAMP-specific (dunce; phosphodiesterase 4D, cAMP specific; Phosphodiesterase 4D, cAMP-specific (dunce; phosphodiesterase 4D, cAMP-specific (phosphodiesterase E3 dunce homolog, Drosophila); testicular tissue protein Li 136

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Gene Aliases: 9630011N22Rik; ACRDYS2; DPDE3; HSPDE4D; PDE43; PDE4D; PDE4DN2; STRK1

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UniProt ID: (Human) Q08499, (Rat) P14270, (Mouse) Q01063

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Entrez Gene ID: (Human) 5144, (Rat) 24627, (Mouse) 238871

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Function(s)

3',5'-cyclic-nucleotide phosphodiesterase activity 3',5'-cyclic-AMP phosphodiesterase activity protein binding drug binding enzyme binding cAMP binding beta-2 adrenergic receptor binding ion channel binding metal ion binding ATPase binding scaffold protein binding cyclic-nucleotide phosphodiesterase activity SH3 domain binding protein domain specific binding ubiquitin protein ligase binding phosphoric diester hydrolase activity hydrolase activity phosphodiesterase

Process(es)

regulation of heart rate cAMP catabolic process smooth muscle contraction aging regulation of receptor activity regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum cAMP-mediated signaling neutrophil chemotaxis regulation of cAMP metabolic process positive regulation of interferon-gamma production positive regulation of interleukin-2 production positive regulation of interleukin-5 production negative regulation of peptidyl-serine phosphorylation multicellular organism growth negative regulation of heart contraction T cell receptor signaling pathway regulation of ryanodine-sensitive calcium-release channel activity establishment of endothelial barrier cellular response to lipopolysaccharide cellular response to epinephrine stimulus adrenergic receptor signaling pathway regulation of cardiac muscle cell contraction adrenergic receptor signaling pathway involved in positive regulation of heart rate regulation of cell communication by electrical coupling involved in cardiac conduction negative regulation of relaxation of cardiac muscle memory regulation of G-protein coupled receptor protein signaling pathway regulation of protein kinase A signaling positive regulation of smooth muscle cell migration lung development cellular protein complex assembly positive regulation of smooth muscle cell proliferation leukocyte migration cellular response to cAMP signal transduction

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PDE4D Polyclonal Antibody

PDE4D Antibody (12918-1-AP) in ICC/IF

Product Details

12918-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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