Invitrogen

PDGF-BB Polyclonal Antibody

View all (7) PDGF-BB antibodies

Questions & Answers

Questions & Answers

PDGF-BB Antibody in Immunocytochemistry (ICC/IF)

PDGF-BB Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 4

PDGF-BB Antibody (PA5-88272) in ICC/IF

Immunofluorescence analysis of PDGF-BB in HeLa cells. Samples were incubated with PDGF-BB Polyclonal antibody (Product # PA5-88272) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

PDGF-BB Antibody in Immunocytochemistry (ICC/IF)

PDGF-BB Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PDGF-BB Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PDGF-BB Antibody in Western Blot (WB)

Product Image Gallery

PDGF-BB Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of PDGF-BB in HeLa cells. Samples were incubated with PDGF-BB Polyclonal antibody (Product # PA5-88272) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

PDGF-BB Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of PDGF-BB in paraffin-embedded human placenta. Samples were incubated with PDGF-BB Polyclonal antibody (Product # PA5-88272) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

PDGF-BB Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of PDGF-BB in paraffin-embedded rat heart. Samples were incubated with PDGF-BB Polyclonal antibody (Product # PA5-88272) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

PDGF-BB Antibody in Western Blot (WB) — Western blot analysis of PDGF-BB in extracts of various cell lines. Samples were incubated with PDGF-BB Polyclonal antibody (Product # PA5-88272) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Product Details

PA5-88272

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

ELISA (ELISA)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 140-241 of human PDGFB (NP_0025991)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.22 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.05% ProClin 300

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2804792

Product Specific Information

Positive Samples: SW480, MCF7, Mouse skeletal muscle

Immunogen sequence: QCRPTQVQLR PVQVRKIEIV RKKPIFKKAT VTLEDHLACK CETVAAARPV TRSPGGSQEQ RAKTPQTRVT IRTVRVRRPP KGKHRKFKHT HDKTALKETL GA

Target Information

The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 7, at sites where this gene and that for COL1A1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans resulting from unregulated expression of growth factor. Two alternatively spliced transcript variants encoding different isoforms have been identified for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Platelet-Derived Growth Factor-BB Inhibits Intervertebral Disc Degeneration via Suppressing Pyroptosis and Activating the MAPK Signaling Pathway.

Frontiers in pharmacology

Zhang W,Gong Y,Zheng X,Qiu J,Jiang T,Chen L,Lu F,Wu X,Cheng F,Hong Z

PA5-88272 was used in Immunohistochemistry, ELISA to indicate that PDGF-BB participates in inhibiting the occurrence and development of IDD by inhibiting pyroptosis and regulating the MAPK signaling pathway.

Tue Feb 01 00:00:00 EST 2022

Platelet-Derived Growth Factor-BB Inhibits Intervertebral Disc Degeneration via Suppressing Pyroptosis and Activating the MAPK Signaling Pathway.

Frontiers in pharmacology

Zhang W,Gong Y,Zheng X,Qiu J,Jiang T,Chen L,Lu F,Wu X,Cheng F,Hong Z

PA5-88272 was used in Immunohistochemistry, ELISA to indicate that PDGF-BB participates in inhibiting the occurrence and development of IDD by inhibiting pyroptosis and regulating the MAPK signaling pathway.

Tue Feb 01 00:00:00 EST 2022

Platelet-Derived Growth Factor-BB Inhibits Intervertebral Disc Degeneration via Suppressing Pyroptosis and Activating the MAPK Signaling Pathway.

Frontiers in pharmacology

Zhang W,Gong Y,Zheng X,Qiu J,Jiang T,Chen L,Lu F,Wu X,Cheng F,Hong Z

PA5-88272 was used in Immunohistochemistry, ELISA to indicate that PDGF-BB participates in inhibiting the occurrence and development of IDD by inhibiting pyroptosis and regulating the MAPK signaling pathway.

Tue Feb 01 00:00:00 EST 2022

Platelet-Derived Growth Factor-BB Inhibits Intervertebral Disc Degeneration via Suppressing Pyroptosis and Activating the MAPK Signaling Pathway.

Frontiers in pharmacology

Zhang W,Gong Y,Zheng X,Qiu J,Jiang T,Chen L,Lu F,Wu X,Cheng F,Hong Z

PA5-88272 was used in Immunohistochemistry, ELISA to indicate that PDGF-BB participates in inhibiting the occurrence and development of IDD by inhibiting pyroptosis and regulating the MAPK signaling pathway.

Tue Feb 01 00:00:00 EST 2022

Platelet-Derived Growth Factor-BB Inhibits Intervertebral Disc Degeneration via Suppressing Pyroptosis and Activating the MAPK Signaling Pathway.

Frontiers in pharmacology

Zhang W,Gong Y,Zheng X,Qiu J,Jiang T,Chen L,Lu F,Wu X,Cheng F,Hong Z

PA5-88272 was used in Immunohistochemistry, ELISA to indicate that PDGF-BB participates in inhibiting the occurrence and development of IDD by inhibiting pyroptosis and regulating the MAPK signaling pathway.

Tue Feb 01 00:00:00 EST 2022

Platelet-Derived Growth Factor-BB Inhibits Intervertebral Disc Degeneration via Suppressing Pyroptosis and Activating the MAPK Signaling Pathway.

Frontiers in pharmacology

Zhang W,Gong Y,Zheng X,Qiu J,Jiang T,Chen L,Lu F,Wu X,Cheng F,Hong Z

PA5-88272 was used in Immunohistochemistry, ELISA to indicate that PDGF-BB participates in inhibiting the occurrence and development of IDD by inhibiting pyroptosis and regulating the MAPK signaling pathway.

Tue Feb 01 00:00:00 EST 2022

Bioinformatics

Protein Aliases: becaplermin; FLJ12858; H-PDGF-BB; pdgf protein; PDGF subunit B; PDGF, B chain; PDGF-2; platelet derived growth factor, B polypeptide; platelet-derived growth factor 2; Platelet-derived growth factor B chain; Platelet-derived growth factor beta polypeptide; platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog); Platelet-derived growth factor subunit B; platelet-derived growth factor, beta polypeptide (oncogene SIS); Platelet-derived growth factor, same as Sis (Simian sarcoma viral oncogene homologue, c-sis); Proto-oncogene c-Sis

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Gene Aliases: c-sis; IBGC5; PDGF-2; PDGF2; PDGFB; SIS; SSV

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UniProt ID: (Human) P01127, (Rat) Q05028

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Entrez Gene ID: (Human) 5155, (Rat) 24628

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Ras guanyl-nucleotide exchange factor activity platelet-derived growth factor receptor binding protein binding collagen binding growth factor activity superoxide-generating NADPH oxidase activator activity chemoattractant activity identical protein binding protein homodimerization activity phosphatidylinositol-4,5-bisphosphate 3-kinase activity protein heterodimerization activity platelet-derived growth factor binding receptor binding growth factor

Process(es)

MAPK cascade response to hypoxia embryonic placenta development positive regulation of endothelial cell proliferation monocyte chemotaxis platelet degranulation positive regulation of glomerular filtration DNA replication protein phosphorylation transforming growth factor beta receptor signaling pathway heart development positive regulation of cell proliferation response to wounding negative regulation of phosphatidylinositol biosynthetic process negative regulation of platelet activation positive regulation of gene expression negative regulation of gene expression regulation of phosphatidylinositol 3-kinase signaling positive regulation of phosphatidylinositol 3-kinase signaling positive regulation of smooth muscle cell migration cell growth peptidyl-serine phosphorylation peptidyl-tyrosine phosphorylation hemopoiesis extracellular matrix organization positive regulation of cell migration positive regulation of protein autophosphorylation negative regulation of protein binding activation of protein kinase activity activation of protein kinase B activity response to estradiol response to insulin positive regulation of metanephric mesenchymal cell migration by platelet-derived growth factor receptor-beta signaling pathway paracrine signaling wound healing response to drug positive regulation of MAP kinase activity positive regulation of MAPK cascade positive regulation of blood vessel endothelial cell migration positive regulation of GTPase activity positive regulation of phosphatidylinositol 3-kinase activity response to estrogen positive regulation of cyclin-dependent protein serine/threonine kinase activity positive regulation of DNA replication positive regulation of mitotic nuclear division negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated phosphatidylinositol phosphorylation platelet-derived growth factor receptor signaling pathway phosphatidylinositol-mediated signaling positive regulation of fibroblast proliferation positive regulation of smooth muscle cell proliferation positive regulation of peptidyl-tyrosine phosphorylation positive chemotaxis positive regulation of chemotaxis positive regulation of cell division cell chemotaxis positive regulation of protein tyrosine kinase activity positive regulation of ERK1 and ERK2 cascade protein kinase C signaling cellular response to growth factor stimulus cellular response to mycophenolic acid positive regulation of glomerular mesangial cell proliferation metanephric glomerular mesangial cell development reactive oxygen species metabolic process positive regulation of calcium ion import positive regulation of hyaluronan biosynthetic process negative regulation of pri-miRNA transcription from RNA polymerase II promoter positive regulation of pri-miRNA transcription from RNA polymerase II promoter positive regulation of vascular smooth muscle cell proliferation positive regulation of vascular associated smooth muscle cell migration negative regulation of vascular smooth muscle cell differentiation positive regulation of vascular smooth muscle cell dedifferentiation positive regulation of reactive oxygen species metabolic process positive regulation of DNA biosynthetic process positive regulation of metanephric mesenchymal cell migration substrate-dependent cell migration blood coagulation response to organic substance response to organic cyclic compound cell projection assembly actin cytoskeleton organization negative regulation of cell migration regulation of cell proliferation positive regulation of fibroblast growth factor receptor signaling pathway positive regulation of mitotic cell cycle, embryonic blood vessel morphogenesis regulation of peptidyl-tyrosine phosphorylation branching involved in salivary gland morphogenesis epithelial cell proliferation involved in salivary gland morphogenesis metanephric glomerular mesangial cell proliferation involved in metanephros development metanephric glomerular endothelium development

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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