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PDGF-C Antibody (MA5-29756) in ICC/IF
Immunofluorescence staining of PDGF-C in HepG2 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with PDGF-C Recombinant Rabbit Monoclonal Antibody (4) (Product # MA5-29756, 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to nucleus and cytoplasm.
Product Details
MA5-29756
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
004
Immunogen
Recombinant Human PDGF-C Protein (Val235-Gly345)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2785579
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
This antibody has specificity for Human PDGF-C.
Target Information
Sphingosine Kinase 2 (Sphk2) catalyzes the phosphorylation of sphingosine to sphingosine 1 phosphate (S1P), an important signaling molecule with intra- and extracellular functions. Inside the cell S1P acts as a signaling molecule like other sphingolipid metabolites like ceramide and sphingosine. S1P has been implicated in regulating cell differentiation, calcium mobilization from intracellular stores, and apoptosis. The cell surface receptors for S1P are the EDG family of G protein-coupled receptors (S1P Receptors).These receptors couple to multiple G proteins (e.g. S1P1 couples to Gi whereas S1P2 and S1P3 couple to Gq, G13 in addition to Gi) and regulate a extremely wide range of cellular events including cell motility, survival, apoptosis, migration and cellcell interaction. Important roles for S1P have also been reported in regulation of cardiogenesis, vascular maturation, oocyte survival, immune cell trafficking, cells of the neuronal system and bone cells. S1P levels are regulated by the activity of Sphk (Sphk1 and Sphk2).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C76851; Fallotein; MGC102297; PDGF-C; Platelet-derived growth factor C; SCDGF; secretory growth factor-like protein; Spinal cord-derived growth factor; VEGF-E
Gene Aliases: FALLOTEIN; PDGFC; SCDGF; UNQ174/PRO200
UniProt ID: (Human) Q9NRA1
Entrez Gene ID: (Human) 56034
activation of transmembrane receptor protein tyrosine kinase activity
central nervous system development
blood coagulation
embryo development
organ morphogenesis
positive regulation of phosphatidylinositol 3-kinase signaling
positive regulation of cell migration
positive regulation of protein autophosphorylation
positive regulation of MAP kinase activity
positive regulation of DNA replication
platelet-derived growth factor receptor signaling pathway
positive regulation of fibroblast proliferation
digestive tract development
regulation of peptidyl-tyrosine phosphorylation
positive regulation of cell division
bone development
positive regulation of ERK1 and ERK2 cascade
cellular response to amino acid stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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