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Invitrogen
PDGF-D Polyclonal Antibody
Advanced Verification
This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 17
PDGF-D Antibody (40-2100) in IHC (P)
Immunohistochemistry analysis of PDGF-D showing staining in the cytoplasm of paraffin-embedded human heart tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, wa... View More
Product Details
40-2100
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide derived from the C-terminal region of the human PDGF-D
Conjugate
Form
Liquid
Concentration
0.25 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2533455
Target Information
The platelet-derived growth factor (PDGF) family consists of four members; PDGF-A, PDGF-B, PDGF-C and PDGF-D (spinal cord-derived growth factor-B or iris-expressed growth factor). PDGF members form disulphide-bonded dimeric isoforms, which are important for growth, survival and function in several types of connective tissue cells. Their biological effects are mediated via two tyrosine kinase receptors, PDGFR-a and PDGFR-b, and PDGF-mediated signaling is critical for development of many organ systems. PDGF-D has a two-domain structure similar to PDGF-C and is secreted as a disulphide-linked homodimer, PDGF-DD. PDGF-D induces cellular transformation and promotes tumor growth by accelerating the proliferation rate of tumor cells and by stimulation of tumor neovascularization. PDGF-D may play a role in the development of brain tumors. The potential oncogenic activity of PDGF-DD may be important for the development and/or progression of prostate cancer. PDGF-D is expressed in fibroblastic adventitial cells, cultured endothelial cells and in a variety of tumor cell lines including those derived from ovarian, renal and lung cancers, as well as from astrocytomas and medulloblastomas. PDGF-D is expressed in the human kidney.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Iris-expressed growth factor; PDGF-D; Platelet-derived growth factor D; platelet-derived growth factor, D polypeptide; SCDGF-B; spinal cord derived growth factor B; Spinal cord-derived growth factor B; spinal cord-derived growth factor-B; spinal-cord derived growth factor-B protein
Gene Aliases: 1110003I09Rik; IEGF; MSTP036; PDGFD; rSCDGF-B; SCDGF-B; SCDGFB; UNQ1899/PRO4345
UniProt ID: (Human) Q9GZP0, (Rat) Q9EQT1, (Mouse) Q925I7
Entrez Gene ID: (Human) 80310, (Rat) 66018, (Mouse) 71785
multicellular organism development
blood coagulation
positive regulation of cell proliferation
positive regulation of phosphatidylinositol 3-kinase signaling
positive regulation of cell migration
positive regulation of protein autophosphorylation
cellular response to platelet-derived growth factor stimulus
positive regulation of MAP kinase activity
platelet-derived growth factor receptor signaling pathway
positive regulation of fibroblast proliferation
positive regulation of smooth muscle cell proliferation
regulation of peptidyl-tyrosine phosphorylation
positive regulation of cell division
cellular response to hydrogen peroxide
positive regulation of ERK1 and ERK2 cascade
cellular response to amino acid stimulus
cellular response to transforming growth factor beta stimulus
positive regulation of smooth muscle cell chemotaxis
positive regulation of glomerular mesangial cell proliferation
positive regulation of monocyte extravasation
cellular process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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