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Invitrogen
PDGFRA Polyclonal Antibody
FIGURE: 1 / 4
PDGFRA Antibody (PA5-34739) in ICC/IF
PDGFRA Polyclonal Antibody detects PDGF Receptor alpha protein at cytoplasm by immunofluorescent analysis. Sample: NIH/3T3 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PDGF Receptor alpha stained by PDGFRA Polyclonal Antibody (Product # PA5-34739) diluted at 1:500. Red: phalloidin, a cytoskeleton marker diluted at 1:200. Blue: Hoechst 33342 staining.
Product Details
PA5-34739
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein fragment contains a sequence corresponding to a region within amino acids 331 and 709 (P16234) of PDGF Receptor alpha
Conjugate
Form
Liquid
Concentration
1.13 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2552091
Product Specific Information
Recommended positive controls: K562, PC-12.
Predicted reactivity: Human (99%), Mouse (91%), Rat (92%), Xenopus laevis (82%), Chicken (86%), Bovine (96%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
PDGFRA is a cell surface receptor tyrosine kinase which binds members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies in knockout mice, where homozygosity is lethal, indicate that the alpha form of the platelet-derived growth factor receptor is particularly important for kidney development since mice heterozygous for the receptor exhibit defective kidney phenotypes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Alpha platelet-derived growth factor receptor; Alpha platelet-derived growth factor receptor precursor (PDGF-R-alpha); Alpha-type platelet-derived growth factor receptor; CD140 antigen-like family member A; CD140a; CD140a antigen; MGC74795; OTTHUMP00000218656; PDGF A-chain; PDGF alpha chain; PDGF Receptor alpha; PDGF-R-alpha; PDGFR-2; pdgfr-a; PDGFR-alpha; PDGFRA/BCR fusion; Platelet-derived growth factor alpha receptor; Platelet-derived growth factor receptor 2; Platelet-derived growth factor receptor alpha; platelet-derived growth factor receptor, alpha polypeptide; rearranged-in-hypereosinophilia-platelet derived growth factor receptor alpha fusion protein
Gene Aliases: AI115593; APDGFR; CD140A; GAS9; PDGFACE; PDGFR-2; PDGFR2; PDGFRA; RHEPDGFRA
UniProt ID: (Human) P16234, (Rat) P20786, (Mouse) P26618
Entrez Gene ID: (Human) 5156, (Rat) 25267, (Mouse) 18595
protein kinase activity
transmembrane receptor protein tyrosine kinase activity
platelet-derived growth factor alpha-receptor activity
vascular endothelial growth factor-activated receptor activity
Ras guanyl-nucleotide exchange factor activity
platelet-derived growth factor receptor binding
protein binding
ATP binding
vascular endothelial growth factor binding
protein homodimerization activity
phosphatidylinositol-4,5-bisphosphate 3-kinase activity
platelet-derived growth factor binding
protein complex binding
phosphatidylinositol 3-kinase binding
protein dimerization activity
nucleotide binding
protein tyrosine kinase activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
transmembrane signal receptor
MAPK cascade
luteinization
cell activation
positive regulation of cytosolic calcium ion concentration
positive regulation of cell proliferation
negative regulation of platelet activation
positive regulation of phospholipase C activity
regulation of phosphatidylinositol 3-kinase signaling
positive regulation of phosphatidylinositol 3-kinase signaling
viral process
peptidyl-tyrosine phosphorylation
positive regulation of cell migration
cellular response to reactive oxygen species
platelet-derived growth factor receptor-alpha signaling pathway
positive regulation of cell proliferation by VEGF-activated platelet derived growth factor receptor signaling pathway
wound healing
positive regulation of GTPase activity
positive regulation of phosphatidylinositol 3-kinase activity
positive regulation of DNA replication
protein autophosphorylation
phosphatidylinositol phosphorylation
platelet-derived growth factor receptor signaling pathway
phosphatidylinositol-mediated signaling
positive regulation of fibroblast proliferation
embryonic digestive tract morphogenesis
embryonic cranial skeleton morphogenesis
embryonic skeletal system morphogenesis
regulation of chemotaxis
cardiac myofibril assembly
cell chemotaxis
retina vasculature development in camera-type eye
positive regulation of ERK1 and ERK2 cascade
platelet aggregation
metanephric glomerular capillary formation
regulation of actin cytoskeleton reorganization
regulation of mesenchymal stem cell differentiation
in utero embryonic development
hematopoietic progenitor cell differentiation
estrogen metabolic process
female gonad development
anatomical structure morphogenesis
response to hormone
organ morphogenesis
response to organic substance
response to inorganic substance
cell migration
signal transduction involved in regulation of gene expression
extracellular matrix organization
lung development
adrenal gland development
male genitalia development
response to estradiol
Leydig cell differentiation
response to cytokine
cellular response to platelet-derived growth factor stimulus
vascular endothelial growth factor signaling pathway
gliogenesis
odontogenesis of dentin-containing tooth
response to estrogen
skeletal system morphogenesis
inner ear development
response to hyperoxia
palate development
face morphogenesis
cellular response to amino acid stimulus
protein phosphorylation
chemotaxis
transmembrane receptor protein tyrosine kinase signaling pathway
multicellular organism development
phosphorylation
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