Invitrogen

PDGFRB Monoclonal Antibody (18A2), PE

1 Reference
View all (90) PDGFRB (CD140b) antibodies
Datasheet
Protocols
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Datasheet
Protocols
Questions & Answers

Product Details

MA1-10102

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

20 µL/1x10^6 cells
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Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

18A2

Immunogen

CD140b-transfected NIH 3T3 cells
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Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra spectra

Form

Liquid

Purification

Size-exclusion chromatography

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

15mM sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11152491

Product Specific Information

This antibody recognizes an extracellular epitope of CD140b / PDGF-RB, the 180-190 kDa beta chain of platelet-derived growth factor receptor, which is widely expressed on a variety of mesenchymal-derived cells and plays pro-proliferative roles.

Target Information

PDGFRB is a receptor tyrosine kinase and has been implicated in atherogenesis and in cell transformation by the SIS oncogene. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. The gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor; all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses PDGFRB (CD140b) to that of the translocation, ETV6, leukemia gene, results in chronic myeloproliferative disorder with eosinophilia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Beta platelet-derived growth factor receptor; Beta-type platelet-derived growth factor receptor; CD140 antigen-like family member B; CD140b; CD140b antigen; PDGF Receptor beta; PDGF-R-beta; PDGFR-1; PDGFR-beta; Platelet-derived growth factor receptor 1; Platelet-derived growth factor receptor beta; platelet-derived growth factor receptor, beta polypeptide

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Gene Aliases: CD140B; IBGC4; IMF1; JTK12; KOGS; PDGFR; PDGFR-1; PDGFR1; PDGFRB; PENTT

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UniProt ID: (Human) P09619

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Entrez Gene ID: (Human) 5159

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Function(s)
protein tyrosine kinase activity platelet activating factor receptor activity platelet-derived growth factor-activated receptor activity platelet-derived growth factor beta-receptor activity Ras guanyl-nucleotide exchange factor activity receptor binding platelet-derived growth factor receptor binding protein binding ATP binding protein kinase binding vascular endothelial growth factor binding phosphatidylinositol 3-kinase binding phosphatidylinositol-4,5-bisphosphate 3-kinase activity platelet-derived growth factor binding transmembrane signal receptor
Process(es)
MAPK cascade glycosaminoglycan biosynthetic process signal transduction G-protein coupled receptor signaling pathway positive regulation of cell proliferation response to toxic substance positive regulation of phospholipase C activity regulation of phosphatidylinositol 3-kinase signaling positive regulation of phosphatidylinositol 3-kinase signaling positive regulation of smooth muscle cell migration cell migration peptidyl-tyrosine phosphorylation positive regulation of cell migration response to estradiol positive regulation of phosphoprotein phosphatase activity response to retinoic acid regulation of actin cytoskeleton organization positive regulation of collagen biosynthetic process response to fluid shear stress cell migration involved in vasculogenesis metanephric mesenchymal cell migration platelet-derived growth factor receptor-beta signaling pathway positive regulation of metanephric mesenchymal cell migration by platelet-derived growth factor receptor-beta signaling pathway aorta morphogenesis cellular response to platelet-derived growth factor stimulus positive regulation of cell proliferation by VEGF-activated platelet derived growth factor receptor signaling pathway wound healing response to hydrogen peroxide negative regulation of apoptotic process positive regulation of MAP kinase activity positive regulation of GTPase activity positive regulation of phosphatidylinositol 3-kinase activity response to estrogen positive regulation of mitotic nuclear division phosphatidylinositol metabolic process protein autophosphorylation phosphatidylinositol phosphorylation platelet-derived growth factor receptor signaling pathway phosphatidylinositol-mediated signaling positive regulation of smooth muscle cell proliferation inner ear development positive regulation of chemotaxis cardiac myofibril assembly response to hyperoxia cell chemotaxis cell migration involved in coronary angiogenesis retina vasculature development in camera-type eye positive regulation of ERK1 and ERK2 cascade smooth muscle cell chemotaxis metanephric mesenchyme development metanephric glomerular mesangial cell proliferation involved in metanephros development metanephric glomerular capillary formation metanephric comma-shaped body morphogenesis metanephric S-shaped body morphogenesis positive regulation of calcium ion import positive regulation of reactive oxygen species metabolic process positive regulation of DNA biosynthetic process
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